Detection of Bacterial Meningitis Pathogens by PCR-Mass Spectrometry in Cerebrospinal Fluid

Jing-Zi, Piao; He, Zhengxin; Chen, Weijun; Jiang, Yongqiang

doi:10.7754/clin.lab.2018.180122

Cited by 4 publications

(2 citation statements)

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“…Moreover,, the mentioned methods have some limitations such as low bacterial titers in CSF fluid, lengthy incubation period of bacterial cultures, cross-reactivity in immunological methods, and antibiotic use by patients prior to CSF sample collection which impairs the culture response and the identification of the pathogen causing bacterial meningitis ( 4 ). Now, molecular methods, such as PCR, are more effective in detecting the possible presence of bacterial DNA in the CSF samples of patients with suspected bacterial meningitis ( 5 ). Standard PCR has the ability to detect possible bacterial DNA in CSF fluid even if the causative agent is not alive.…”

Section: Introductionmentioning

confidence: 99%

Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K , Streptococcus agalactiae, and Streptococcus pneumoniae

Hemmati¹,

Nikkhahi²,

Javadi³

et al. 2021

IJM

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Background and Objectives: Neisseria meningitidis, Escherichia coli K , Streptococcus agalactiae, and Streptococcus pneumoniae cause 90% of bacterial meningitis. Almost all infected people die or have irreversible neurological complica- tions. Therefore, it is essential to have a diagnostic kit with the ability to quickly detect these fatal infections. Materials and Methods: The project involved 212 patients from whom cerebrospinal fluid samples were obtained. After total genome extraction and performing multiplex quantitative polymerase chain reaction (qPCR), the presence or absence of each infectious factor was determined by comparing with standard strains. Results: The specificity, sensitivity, positive predictive value, and negative predictive value calculated were 100%, 92.9%, 50%, and 100%, respectively. So, due to the high specificity and sensitivity of the designed primers, they can be used instead of bacterial culture that takes at least 24 to 48 hours. Conclusion: The remarkable benefit of this method is associated with the speed (up to 3 hours) at which the procedure could be completed. It is also worth noting that this method can reduce the personnel unintentional errors which may occur in the laboratory. On the other hand, as this method simultaneously identifies four common factors that cause bacterial meningitis, it could be used as an auxiliary method diagnostic technique in laboratories particularly in cases of emergency medicine.

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Section: Introductionmentioning

confidence: 99%

Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K , Streptococcus agalactiae, and Streptococcus pneumoniae

Hemmati¹,

Nikkhahi²,

Javadi³

et al. 2021

IJM

View full text Add to dashboard Cite

show abstract

“…Yazarlar, özellikle antibiyotik ntedavisi alan hastalarda bile testin bakteriyel menenjit etkenini hızlı ve doğru olarak saptadığını, klinik tanı ve epidemiyolojik önlemleri iyileştirdiğini bildirmişlerdir (4). PZR gibi moleküler yöntemlerin bakteriyel menenjite yönelik erken antibiyotik tedavi başlanan, tedavi öncesi kültür için örnek alınmadığından tedavi başlandıktan sonra alınan kültürde üreme saptanmayan olgularda pozitif sonuç vermesi avantajıdır(5). PZR yönteminin kültürden daha önce sonuçlanması da bir diğer avantajıdır(6).…”

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Polimeraz zincir reaksiyonu ile tanısı konulan ve komplikasyonla seyreden bir pnömokok menenjiti olgusu

Gelişigüzel

Cesur

Arslan

et al. 2021

Journal of Medicine and Palliative Care

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Bakteriyel menenjitlerin erken tanısı ve tedavisinde kültür sonuçları en erken 24-48 saatte çıktığından ötürü, klinik bulgularla birlikte beyin omurilik sıvısı (BOS)'nda hücre sayımı, BOS'un biyokimyasal özellikleri ve BOS'un Gram boyama ile incelenmesi büyük önem taşımaktadır. Özellikle menenjitlerin infeksiyon acili olması nedeniyle kültür için BOS ve kan örneği alınmadan ampirik tedavinin erken başlandığı veya hastaların önceden antibiyotik tedavisi aldığı durumlarda kültürde etkenin saptanma olasılığı azalır veya ortadan kalkar. Bu gibi durumlarda BOS'un Gram boyaması, BOS' da bakteri antijenlerinin araştırılması yanı sıra son yıllarda polieraz zincir reaksiyonu ile de bakteri DNA'sı araştırılarak da tanıya gidilebilmektedir. Burada, menenjit ön tanısıyla BOS örneği alınmadan seftriakson tedavisi başlanan bu nedenle kültürde etkenin saptanmadığı, ancak; multipleks PZR yöntemiyle BOS' da Streptococcus pneumoniae pozitif saptanan 46 yaşında bir kadın hasta sunularak literatür gözden geçirildi. Olguda uygun antimikrobiyal tedaviye rağmen, pnömokok menenjiti komplikasyonu olarak superior sagittal sinüs ve bilateral transverslerde yaygın serebral trombüs gelişti.

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Point-of-care multiplexed diagnosis of meningitis using the FilmArray® ME panel technology

Vincent

Zandotti

Baron

et al. 2020

Eur J Clin Microbiol Infect Dis

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Detection of Bacterial Meningitis Pathogens by PCR-Mass Spectrometry in Cerebrospinal Fluid

Abstract: PCR-Mass could be used for the molecular detection of bacterial meningitis and tuberculosis, especially when early antibiotic treatment has been administered to the suspected patients.

Cited by 4 publications

References 0 publications

Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K , Streptococcus agalactiae, and Streptococcus pneumoniae

Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K , Streptococcus agalactiae, and Streptococcus pneumoniae

Polimeraz zincir reaksiyonu ile tanısı konulan ve komplikasyonla seyreden bir pnömokok menenjiti olgusu

Point-of-care multiplexed diagnosis of meningitis using the FilmArray® ME panel technology

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