Cigarette smoking and risk of Behcet’s disease: a propensity score matching analysis

Mahdavi, Aida Malek; Khabbazi, Alireza; Yaaghoobian, Barmak; Ghojazadeh, Morteza; Agamohammadi, Ramin; Kheyrollahiyan, Atefeh; Rashtchizadeh, Nadereh

doi:10.1080/14397595.2018.1493065

Cited by 23 publications

(19 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 The etiology of BD is unknown. However, environmental factors especially microbial agents, 2,3 vitamin D deficiency, 4 and possibly smoking, 5 genetic factors, [6][7][8][9][10][11] and epigenetic changes 12 contribute to disease development.…”

Section: Introductionmentioning

confidence: 99%

The expression and methylation status of vitamin D receptor gene in Behcet's disease

Shirvani

Nouri

Sakhinia

et al. 2019

Immunity Inflam & Disease

Self Cite

View full text Add to dashboard Cite

Introduction Vitamin D has important roles as a natural immune modulator via regulating the expression of genes which have been implicated in the pathophysiology of autoimmune diseases. Vitamin D function and its deficiency have been linked to a wide range of metabolic disorders including disorders of calcium metabolism, malignant, cardiovascular, infectious, neuromuscular, and inflammatory diseases. Environmental factors, genetic factors, and epigenetic changes contribute to Behcet's disease (BD) development. The aim of our study was to analyze the expression level and methylation status of the vitamin D receptor (VDR) gene promoter in the peripheral blood mononuclear cells (PBMCs) of patients with BD. Methods In a case‐control study, 48 Iranian Azeri patients with BD and 60 age‐, sex‐ and ethnically‐matched healthy controls were included. Venous blood samples were collected and PBMCs were isolated by Ficoll protocol. The DNA and RNA were subsequently extracted. Promoter methylation levels were evaluated by MeDIP‐quantitative polymerase chain reaction (qPCR). The expression of VDR was evaluated by real‐time PCR. Results The results of quantitative real‐time PCR analysis showed that the level of VDR expression in patients with BD was significantly lower than the control group (P = .013). There was no significant difference in the level of DNA methylation in the BD and control groups (P > .05). As the results show, the expression level of VDR gene was significantly different between female and male in the patient group (P = .001). VDR gene expression was significantly higher in subjects with phlebitis. No correlation was observed between VDR gene expression rate and BD activity. Conclusion VDR gene expression decreased in patients with BD. However, there is no suggestion evidence that the expression level of VDR is regulated by a unique DNA methylation mechanism. No correlation exists between VDR gene expression and BD activity.

show abstract

Section: Introductionmentioning

confidence: 99%

The expression and methylation status of vitamin D receptor gene in Behcet's disease

Shirvani

Nouri

Sakhinia

et al. 2019

Immunity Inflam & Disease

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, the risk of BD decreased in current smokers, especially in adults younger than 40 years, regardless of the amount of smoking. However, smoking remains an important risk factor for many diseases and it has been reported that smoking increases vascular insufficiency and disease activity in ex‐smokers . Consequently, an indirect or direct unfavourable effect of smoking on patients with BD is expected, and smoking as a therapeutic approach does not seem advisable.…”

Section: Discussionmentioning

confidence: 99%

“…However, smoking remains an important risk factor for many diseases and it has been reported that smoking increases vascular insufficiency 10 and disease activity in exsmokers. 9 Consequently, an indirect or direct unfavourable effect of smoking on patients with BD is expected, and smoking as a therapeutic approach does not seem advisable. Given the harmful effects of smoking and the lack of clear benefits of smoking in BD development, we do not consider smoking as a prevention or treatment option for BD.…”

Section: Discussionmentioning

confidence: 99%

“…3 Although the aetiology of BD has not been not fully established, several genome-wide association studies confirmed the association of BD with HLA-B*51, and ERAP1, IL10, IL23R, CCR1, STAT4, KLRC4, GIMAP2/GIMAP4 and UBAC2 genes. 4 Epigenetic alterations have also been reported in patients with BD, 5 and environmental factors such as microorganisms [6][7][8] and smoking [9][10][11][12][13][14] are known to be associated with BD. BD is a significant disease that greatly affects patient quality of life.…”

Section: Introductionmentioning

confidence: 99%

“…10,12-14,16-,19 However, other authors reported that smoking has detrimental effects on BD. 9,11,20,21 Some studies have found no association between BD and smoking. 22 Smoking is one of the most prevalent addictive habits that result in inflammatory reactions.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Association between smoking and Behçet's disease: a nationwide population‐based study in Korea

Lee

et al. 2019

Acad Dermatol Venereol

View full text Add to dashboard Cite

Background There have been conflicting results about the association between Behçet's disease and smoking. Smoking has been reported to be a protective factor for Behçet's disease, whereas smoking may have a role in triggering Behçet's disease. Objectives The aim of this study was to investigate the incidence of Behçet's disease in Korea according to smoking status using nationwide population data. Methods We analysed clinical data from individuals 20 years of age and older who received a health examination arranged by the Korean national insurance programme between 2009 and 2012. The incidence of Behçet's disease was analysed according to smoking status reported by individuals during their health examination. Newly diagnosed cases of Behçet's disease were identified using claims data from baseline to the date of diagnosis or until 31 December 2016. Results The risk of Behçet's disease was lower in current smokers compared with never‐smokers regardless of the amount and duration of smoking. The decreased risk of Behçet's disease in current smoker persisted after adjusting for age, sex, regular exercise, drinking status, BMI, diabetes mellitus, hypertension, and dyslipidaemia, history of stroke and/or history of ischaemic heart diseases. Limitations Genetic susceptibility or family history of Behçet's disease was not considered. Conclusions This study found a decreased incidence of Behçet's disease in current smokers compared with never‐smokers. Further investigation of the pathophysiology responsible for the negative association between smoking and Behçet's disease is needed.

show abstract

Evaluation of DNA methylation status of toll‐like receptors 2 and 4 promoters in Behcet's disease

Kolahi

Rashtchizadeh

Mahdavi

et al. 2020

The Journal of Gene Medicine

Self Cite

View full text Add to dashboard Cite

Background: Altered innate immune function plays an important role in the initiation of inflammatory response in Behcet's disease (BD). Toll-like receptors (TLRs) are the master regulators of the innate immune system. Because the role of TLRs remains unknown in the pathogenesis of BD, the present study aimed to evaluate the expression levels and methylation status of the TLR2 and TLR4 promoters in patients with BD. Methods: In the present study, Iranian Azeri BD patients (n = 47) with an active (n = 22) and inactive (n = 25) period, and healthy controls (n = 61), were matched according to age, sex and ethnicity. TLR2 and TLR4 genes promoter CpG islands were predicted with the Eukaryotic Promoter Database (https://epd.vital-it.ch). Methylated DNA immunoprecipitation (MeDIP) was conducted. Results: The results showed that mRNA of TLR4 was significantly increased in the peripheral blood mononuclear cells (PBMCs) of BD patients with an active phase compared to the control group. Differences in mRNA of TLR4 between the inactive BD and control groups were not significant. Differences in TLR2 mRNA levels in the PBMCs of the active and inactive phase BD and control groups were not significant. The methylation rate of TLR4 gene promoter was significantly lower in the active and inactive BD groups compared to the control group. The difference between the active and inactive BD groups was not significant. There was no significant difference in the methylation rates of the TLR2 gene between studied groups. Conclusions: Our preliminary findings suggest that the hypomethylation of TLR4 gene may be involved in the pathogenesis of BD via increasing TLR4 expression.

show abstract

Cigarette smoking and risk of Behcet’s disease: a propensity score matching analysis

Abstract: Smoking is not a significant risk factor for BD.

Cited by 23 publications

References 41 publications

The expression and methylation status of vitamin D receptor gene in Behcet's disease

The expression and methylation status of vitamin D receptor gene in Behcet's disease

Association between smoking and Behçet's disease: a nationwide population‐based study in Korea

Evaluation of DNA methylation status of toll‐like receptors 2 and 4 promoters in Behcet's disease

Contact Info

Product

Resources

About