The remarkable plethora of infestation-responsive Q-type C2H2 transcription factors in potato

Lawrence, Susan D.; Novak, Nicole G.

doi:10.1186/s13104-018-3503-6

Cited by 4 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…C2H2 ZnFPs have been previously characterized in rice and function in development ( Liu et al., 2018 ; Zhuang et al., 2020 ) and tolerance to abiotic stress, including drought, salt, and acidic soils ( Iuchi et al., 2008 ; Xu et al., 2008 ; Sun et al., 2010 ). A growing number of studies have documented the contribution of C2H2 ZnFPs to both abiotic and biotic stress tolerance ( Kim et al., 2004 ; Shi et al., 2014 ; Yu et al., 2016 ; Lawrence and Novak, 2018 ; Yin et al., 2020 ; Zhao et al., 2020 ). Furthermore, the potential use of C2H2 ZnFPs in enhancing pathogen resistance in transgenic plants has been successfully demonstrated using the ZFPs CaPIF1 and CaZFP, which were originally identified in pepper ( Kim et al., 2004 ; Oh et al., 2005 ; Shi et al., 2014 ).…”

Section: Discussionmentioning

confidence: 99%

A Xanthomonas transcription activator-like effector is trapped in nonhost plants for immunity

Haq

et al. 2022

Plant Communications

View full text Add to dashboard Cite

Xanthomonas oryzae pv. oryzae ( Xoo ), the causal agent of bacterial leaf blight in rice, delivers transcription activator-like effector (TALE) proteins into host cells to activate susceptibility or resistance ( R ) genes that promote disease or immunity, respectively. Nonhost plants serve as potential reservoirs of R genes; consequently, nonhost R genes may trap TALEs to trigger an immune response. In this study, we screened 17 Xoo TALEs for their ability to induce a hypersensitive response (HR) in the nonhost plant Nicotiana benthamiana ( Nb ); only AvrXa10 elicited an HR when transiently expressed in Nb . The HR generated by AvrXa10 required both the central repeat region and the activation domain, suggesting a specific interaction between AvrXa10 and a potential R -like gene in nonhost plants. Evans blue staining and ion leakage measurements confirmed that the AvrXa10-triggered HR was a form of cell death, and the transient expression of AvrXa10 in Nb induced immune responses. Genes targeted by AvrXa10 in the Nb genome were identified by transcriptome profiling and prediction of effector binding sites. Using several approaches ( in vivo reporter assays, electrophoretic mobility-shift assays, targeted designer TALEs, and on-spot gene silencing), we confirmed that AvrXa10 targets NbZnFP1 , a C2H2-type zinc finger protein that resides in the nucleus. Functional analysis indicated that overexpression of NbZnFP1 and its rice orthologs triggered cell death in rice protoplasts. An NbZnFP1 ortholog was also identified in tomato and was specifically activated by AvrXa10. These results demonstrate that NbZnFP1 is a nonhost R gene that traps AvrXa10 to promote plant immunity in Nb .

show abstract

Section: Discussionmentioning

confidence: 99%

A Xanthomonas transcription activator-like effector is trapped in nonhost plants for immunity

Haq

et al. 2022

Plant Communications

View full text Add to dashboard Cite

show abstract

“…The Q-type ZFPs contain a domain consisting of approximately 25 amino acids and two conserved Cys and His residues, with a consensus sequence of CX2-4CX3FX3QAL-GGHX3-5H [29]. In total, 52 Q-type C2H2 VvZFPs, 54 C2H2 Q-type VrZFPs, and 55 Qtype C2H2 VaZFPs were identified in the genomes of V. vinifera PN40024, V. riparia, V. amurensis, respectively, and these genes have also been identified in other plants [23,33,34]. Analysis of the physicochemical properties of VvZFPs, VrZFPs, and VaZFPs…”

Section: Identification Of Q-type C2h2 Zfps In Grapesmentioning

confidence: 99%

“…The Q-type ZFPs contain a domain consisting of approximately 25 amino acids and two conserved Cys and His residues, with a consensus sequence of CX2-4CX3FX3QALGG-HX3-5H [29]. In total, 52 Q-type C2H2 VvZFPs, 54 C2H2 Q-type VrZFPs, and 55 Q-type C2H2 VaZFPs were identified in the genomes of V. vinifera PN40024, V. riparia, V. amurensis, respectively, and these genes have also been identified in other plants [23,33,34]. Analysis of the physicochemical properties of VvZFPs, VrZFPs, and VaZFPs revealed similarity between these genes; for example, the CDS lengths of VvZFPs, VaZFPs and VrZFPs were found to be in the range 468-1830 bp, 474-2583 bp, and 396-1836 bp, respectively, and their pI was found to be in the range 4.79-9.58, 4.94-10, and 4.78-9.3, respectively.…”

Section: Identification Of Q-type C2h2 Zfps In Grapesmentioning

confidence: 99%

Genome-Wide Identification and Analysis of the Genes Encoding Q-Type C2H2 Zinc Finger Proteins in Grapevine

Chu,

Wang,

et al. 2023

IJMS

View full text Add to dashboard Cite

Q-type C2H2 zinc finger proteins (ZFPs), the largest family of transcription factors, have been extensively studied in plant genomes. However, the genes encoding this transcription factor family have not been explored in grapevine genomes. Therefore, in this study, we conducted a genome-wide identification of ZFP genes in three species of grapevine, namely Vitis vinifera, Vitis riparia, and Vitis amurensis, based on the sequence databases and phylogenetic and their conserved domains. We identified 52, 54, and 55 members of Q-type C2H2 ZFPs in V. vinifera, V. riparia, and V. amurensis, respectively. The physical and chemical properties of VvZFPs, VrZFPs, and VaZFPs were examined. The results showed that these proteins exhibited differences in the physical and chemical properties and that they all were hydrophobic proteins; the instability index showed that the four proteins were stable. The subcellular location of the ZFPs in the grapevine was predicted mainly in the nucleus. The phylogenetic tree analysis of the amino acid sequences of VvZFP, VaZFP, VrZFP, and AtZFP proteins showed that they were closely related and were divided into six subgroups. Chromosome mapping analysis showed that VvZFPs, VrZFPs, and VaZFPs were unevenly distributed on different chromosomes. The clustered gene analysis showed that the motif distribution was similar and the sequence of genes was highly conserved. Exon and intron structure analysis showed that 118 genes of ZFPs were intron deletion types, and the remaining genes had variable numbers of introns, ranging from 2 to 15. Cis-element analysis showed that the promoter of VvZFPs contained multiple cis-elements related to plant hormone response, stress resistance, and growth, among which the stress resistance elements were the predominant elements. Finally, the expression of VvZFP genes was determined using real-time quantitative PCR, which confirmed that the identified genes were involved in response to methyl jasmonate (MeJA), abscisic acid (ABA), salicylic acid (SA), and low-temperature (4 °C) stress. VvZFP10-GFP and VvZFP46-GFP fusion proteins were localized in the nucleus of tobacco cells, and VvZFP10 is the most responsive gene among all VvZFPs with the highest relative expression level to MeJA, ABA, SA and low-temperature (4 °C) stress. The present study provides a theoretical basis for exploring the mechanism of response to exogenous hormones and low-temperature tolerance in grapes and its molecular breeding in the future.

show abstract

“…The EAR motif is hydrophobic and is thought to keep the zinc finger domain folded [15]. The most common types of the EAR motif are "LXLXL" and "DLNXXP" (where X represents any amino acid) [16,17].…”

mentioning

confidence: 99%

Identification of C2H2 zinc finger genes through genome-wide association study and functional analyses of LkZFPs in response to stresses in Larix kaempferi

Shao

Huang

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

Background C2H2 zinc finger proteins (C2H2-ZFPs), one of the largest transcription factors, play a variety of roles in plant development and growth as well as stress response. While, the evolutionary history and expression profile of the C2H2-ZFP genes in Larix kaempferi (LkZFPs) have not been reported so far. Results In this study, the whole genome of the LkZFPs was identified and characterized, including physicochemical properties, phylogenetic relationships, conservative motifs, the promoter cis-elements and Gene Ontology (GO) annotation. We identified 47 LkZFPs and divided them into four subfamilies based on phylogenetic analysis and conserved motifs. Subcellular localization prediction showed that most of the LkZFPs were located in the nucleus. Promoter cis-element analysis suggested that the LkZFPs may be involved in the regulation of stress responses. Moreover, Real-time quantitative PCR (RT-qPCR) results showed that Q-type LkZFP genes were involved in the response to abiotic stress, such as salt, drought and hormone stresses. Subcellular localization results showed that LkZFP7 and LkZFP37 were located in the nucleus, LkZFP32 was located in both cytoplasm and nucleus. Conclusion The identification and functional analysis of LkZFPs suggested that some LkZFP genes might play important roles in coping with both biological and abiotic stresses. These results could further increase understanding of the function of the LkZFPs, and provide some research direction and theoretical support.

show abstract

The remarkable plethora of infestation-responsive Q-type C2H2 transcription factors in potato

Cited by 4 publications

References 31 publications

A Xanthomonas transcription activator-like effector is trapped in nonhost plants for immunity

A Xanthomonas transcription activator-like effector is trapped in nonhost plants for immunity

Genome-Wide Identification and Analysis of the Genes Encoding Q-Type C2H2 Zinc Finger Proteins in Grapevine

Identification of C2H2 zinc finger genes through genome-wide association study and functional analyses of LkZFPs in response to stresses in Larix kaempferi

Contact Info

Product

Resources

About