Metabolomics data of Mitragyna speciosa leaf using LC-ESI-TOF-MS

Veeramohan, Rubashiny; Azizan, Kamalrul Azlan; Aizat, Wan Mohd; Goh, Hoe-Han; Mansor, Sharif Mahsufi; Yusof, Nur Sabrina Mohd; Baharum, Syarul Nataqain; Ng, Chyan Leong

doi:10.1016/j.dib.2018.04.001

Cited by 15 publications

(19 citation statements)

References 9 publications

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“…The scan range was from 100–1000 m / z . Data processing was performed using the software Data Analysis 4.0 and Profile Analysis (Bruker Daltonics) [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Metabolic Profile of Scytalidium parasiticum-Ganoderma boninense Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity

Ahmad

Lim²,

Marzuki³

et al. 2020

Molecules

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In solving the issue of basal stem rot diseases caused by Ganoderma, an investigation of Scytalidium parasiticum as a biological control agent that suppresses Ganoderma infection has gained our interest, as it is more environmentally friendly. Recently, the fungal co-cultivation has emerged as a promising method to discover novel antimicrobial metabolites. In this study, an established technique of co-culturing Scytalidium parasiticum and Ganoderma boninense was applied to produce and induce metabolites that have antifungal activity against G. boninense. The crude extract from the co-culture media was applied to a High Performance Liquid Chromatography (HPLC) preparative column to isolate the bioactive compounds, which were tested against G. boninense. The fractions that showed inhibition against G. boninense were sent for a Liquid Chromatography-Time of Flight-Mass Spectrometry (LC-TOF-MS) analysis to further identify the compounds that were responsible for the microbicidal activity. Interestingly, we found that eudistomin I, naringenin 7-O-beta-D-glucoside and penipanoid A, which were present in different abundances in all the active fractions, except in the control, could be the antimicrobial metabolites. In addition, the abundance of fatty acids, such as oleic acid and stearamide in the active fraction, also enhanced the antimicrobial activity. This comprehensive metabolomics study could be used as the basis for isolating biocontrol compounds to be applied in oil palm fields to combat a Ganoderma infection.

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“…The scan range was from 100–1000 m / z . Data processing was performed using the software Data Analysis 4.0 and Profile Analysis (Bruker Daltonics) [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Metabolic Profile of Scytalidium parasiticum-Ganoderma boninense Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity

Ahmad

Lim²,

Marzuki³

et al. 2020

Molecules

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“…Other compounds, such as flavonoids, terpenoid saponins, polyphenols, and various glycosides are also present [25]. Veeramohan et al [31] performed a metabolomics study using the mature leaves of the green variety of Mitragyna speciosa in order to obtain a more complete profile of kratom’s secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

Mitragyna speciosa: Clinical, Toxicological Aspects and Analysis in Biological and Non-Biological Samples

et al. 2019

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The abuse of psychotropic substances is a well-known phenomenon, and many of them are usually associated with ancestral traditions and home remedies. This is the case of Mitragyna speciosa (kratom), a tropical tree used to improve work performance and to withstand great heat. According to several published studies, the main reasons for kratom consumption involve improving sexual performance and endurance, but also social and recreational uses for the feeling of happiness and euphoria; it is also used for medical purposes as a pain reliever, and in the treatment of diarrhea, fever, diabetes, and hypertension. However, this plant has gained more popularity amongst young people over the last years. Since it is available on the internet for purchase, its use is now widely as a drug of abuse, namely as a new psychoactive substance, being a cheaper alternative to opioids that does not require medical prescription in most countries. According to internet surveys by the European Monitoring Centre for Drugs and Drug Addiction in 2008 and 2011, kratom was one of the most widely supplied new psychoactive substances. The composition of kratom is complex; in fact, more than 40 different alkaloids have been identified in Mitragyna speciosa so far, the major constituent being mitragynine, which is exclusive to this plant. Besides mitragynine, alkaloids such as corynantheidine and 7-hydroxamitragynine also present pharmacological effects, a feature that may be attributed to the remaining constituents as well. The main goal of this review is not only to understand the origin, chemistry, consumption, and analytical methodologies for analysis and mechanism of action, but also the use of secondary metabolites of kratom as therapeutic drugs and the assessment of potential risks associated with its consumption, in order to aid health professionals, toxicologists, and police authorities in cases where this plant is present.

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“…Raw material in “d format” was supplied to Bruker Compass Data Analysis Viewer version 4.2 (Bruker Daltonics, Bremen, Germany) and imported into the Profile Analysis 2.0 data bucketing software (Bruker Daltonics, Bremen, Germany) ( Mamat et al, 2018 ; Veeramohan et al, 2018 ). The parameters for compound detecting were: signal/noise threshold: 5; correlation coefficient: 0.7; minimum compound length: 8; smoothing width: 2.…”

Section: Methodsmentioning

confidence: 99%

Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential

Ahmad

Zin

Mazlan

et al. 2021

PeerJ

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Background Antiplasmodial drug discovery is significant especially from natural sources such as plant bacteria. This research aimed to determine antiplasmodial metabolites of Streptomyces spp. against Plasmodium falciparum 3D7 by using a metabolomics approach. Methods Streptomyces strains’ growth curves, namely SUK 12 and SUK 48, were measured and P. falciparum 3D7 IC50 values were calculated. Metabolomics analysis was conducted on both strains’ mid-exponential and stationary phase extracts. Results The most successful antiplasmodial activity of SUK 12 and SUK 48 extracts shown to be at the stationary phase with IC50 values of 0.8168 ng/mL and 0.1963 ng/mL, respectively. In contrast, the IC50 value of chloroquine diphosphate (CQ) for antiplasmodial activity was 0.2812 ng/mL. The univariate analysis revealed that 854 metabolites and 14, 44 and three metabolites showed significant differences in terms of strain, fermentation phase, and their interactions. Orthogonal partial least square-discriminant analysis and S-loading plot putatively identified pavettine, aurantioclavine, and 4-butyldiphenylmethane as significant outliers from the stationary phase of SUK 48. For potential isolation, metabolomics approach may be used as a preliminary approach to rapidly track and identify the presence of antimalarial metabolites before any isolation and purification can be done.

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Metabolomics data of Mitragyna speciosa leaf using LC-ESI-TOF-MS

Cited by 15 publications

References 9 publications

Metabolic Profile of Scytalidium parasiticum-Ganoderma boninense Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity

Metabolic Profile of Scytalidium parasiticum-Ganoderma boninense Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity

Mitragyna speciosa: Clinical, Toxicological Aspects and Analysis in Biological and Non-Biological Samples

Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential

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