Regeneration of the oesophageal muscle layer from oesophagus acellular matrix scaffold using adipose-derived stem cells

Wang, Fang; Maeda, Yasuko; Zachar, Vladimír; Ansari, Tahera; Emmersen, Jeppe

doi:10.1016/j.bbrc.2018.06.014

Cited by 21 publications

(10 citation statements)

References 18 publications

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“…Additionally, no brown deposits were seen on H&E stained sections either prior or post de-cellularisation.. Our previous study on porcine oesophagus scaffolds, also showed a pale colouration of the scaffold tissue following complete de-cellularisation which was confirmed both molecularly and histologically. [27] Porcine livers were harvested using a similar approach to that employed for harvesting human livers for transplantation. The liver's inherent vascular circulatory system (HPV and HA) was used to deliver decellularising reagents into the tissue under peristaltic flow; the latter chosen in order to mimics normal liver physiology.…”

Section: Discussionmentioning

confidence: 99%

Development and Characterization of a Porcine Liver Scaffold

Ansari

Southgate

Obiri-Yeboa

et al. 2020

Stem Cells and Development

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The growing number of patients requiring liver transplantation for chronic liver disease cannot be currently met due to a shortage in donor tissue. As such, alternative tissue engineering approaches combining the use of acellular biological scaffolds and different cell populations (hepatic or progenitor) are being explored to augment the demand for functional organs. Our goal was to produce a clinically relevant sized scaffold from a sustainable source within 24 hours, whilst preserving the extra cellular matrix (ECM) to facilitate cell repopulation at a later stage. Whole porcine livers underwent perfusion de-cellularisation via the hepatic artery and hepatic portal vein using a combination of saponin, sodium deoxycholate (SOC) and deionised water washes resulting in an acellular scaffold with an intact vasculature and preserved ECM. Molecular and immuno-histochemical analysis (collagen I and IV and laminin) showed complete removal of any DNA material, together with excellent retention of glycosaminoglycans and collagen. FTIR analysis showed both absence of nuclear material and removal of any detergent residue, which was successfully achieved after additional ethanol gradient washes.Samples of the de-cellularised scaffold were assessed for cytotoxicity by seeding with porcine adipose derived mesenchymal stem cells in vitro, these cells over a 10 day period showed attachment and proliferation. Perfusion of the vascular tree with contrast media followed by CT imaging showed an intact vascular network. In vivo implantation of whole intact non-seeded livers, into a porcine model (as auxiliary graft) showed uniform perfusion macroscopically and histologically. Using this method, it is possible to create an acellular, clinically sized, liver scaffold with intact vasculature in less than 24 hours.

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Section: Discussionmentioning

confidence: 99%

Development and Characterization of a Porcine Liver Scaffold

Ansari

Southgate

Obiri-Yeboa

et al. 2020

Stem Cells and Development

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“…However, these conventional reconstruction procedures are associated with a high risk of failure due to fatal complications and morbidity 1,26,27 . Thus, artificial grafts that are similar to esophageal tissues made using tissue engineering and regenerative medicine have emerged as alternatives 3,4,7,8 . Several studies have reported on the reconstruction with artificial esophagi using various materials in an attempt to determine the ideal platform.…”

Section: Discussionmentioning

confidence: 99%

“…However, conventional methods for reconstruction that use the stomach, jejunum, or colon are invasive and can cause morbidities and mortality 1,2 . Hence, tissue engineering of an artificial esophagus that is similar to the native esophagus is emerging as an alternative 3,4 …”

Section: Introductionmentioning

confidence: 99%

Experimental investigation of esophageal reconstruction with electrospun polyurethane nanofiber and 3D printing polycaprolactone scaffolds using a rat model

Park

Kim

et al. 2020

Head & Neck

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Background We evaluated the outcome of esophageal reconstructions using tissue‐engineered scaffolds. Method Partial esophageal defects were reconstructed with the following scaffolds; animals were grouped (n = 7 per group) as follows: (a) normal rats; (b) rats implanted with three‐dimensional printing (3DP) polycaprolactone (PCL) scaffolds; (c) with human adipose‐derived mesenchymal stem cell (ADSC)‐seeded 3DP PCL scaffolds; (d) with polyurethane (PU)‐nanofiber(Nf) scaffolds; and (e) with ADSC‐seeded PU‐Nf scaffolds. Results The esophageal defects were successfully repaired; however, muscle regeneration was greater in the 3DP PCL + ADSC groups than in the PU‐Nf + ADSC groups (P < .001). Regeneration of the epithelium was greater in PU‐Nf and PU‐Nf + ADSC groups than in the 3DP PCL and 3DP PCL + ADSC groups (P < .001). Conclusion A tendency for more re‐epithelization was observed with the PU‐Nf scaffolds, while more muscle regeneration was achieved with the 3DP PCL scaffolds.

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“…There are examples of combining 3D-printing with medical imaging techniques, such as using computer tomography (CT) and MRI to reconstruct replicas of the entire bile duct [ 95 ], but these also lack the addition of sphincter structures. Promising progress has been made in the research of tissue-engineered heart valves [ 96 ], which share some similarities in structural functionality to the neuromuscular Oddi sphincter and may serve as an appropriate source of inspiration to inform the future design of an artificial bile ducts with Oddi sphincter substitutes in the form of bionic valves. Previously, hypoxic pre-conditioning of stem cells seeded onto esophageal acellular matrix scaffolds achieved regeneration of esophageal muscle layers [ 97 ].…”

Section: Future Research Directionsmentioning

confidence: 99%

Progress and Current Limitations of Materials for Artificial Bile Duct Engineering

et al. 2021

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Bile duct injury (BDI) and bile tract diseases are regarded as prominent challenges in hepatobiliary surgery due to the risk of severe complications. Hepatobiliary, pancreatic, and gastrointestinal surgery can inadvertently cause iatrogenic BDI. The commonly utilized clinical treatment of BDI is biliary-enteric anastomosis. However, removal of the Oddi sphincter, which serves as a valve control over the unidirectional flow of bile to the intestine, can result in complications such as reflux cholangitis, restenosis of the bile duct, and cholangiocarcinoma. Tissue engineering and biomaterials offer alternative approaches for BDI treatment. Reconstruction of mechanically functional and biomimetic structures to replace bile ducts aims to promote the ingrowth of bile duct cells and realize tissue regeneration of bile ducts. Current research on artificial bile ducts has remained within preclinical animal model experiments. As more research shows artificial bile duct replacements achieving effective mechanical and functional prevention of biliary peritonitis caused by bile leakage or obstructive jaundice after bile duct reconstruction, clinical translation of tissue-engineered bile ducts has become a theoretical possibility. This literature review provides a comprehensive collection of published works in relation to three tissue engineering approaches for biomimetic bile duct construction: mechanical support from scaffold materials, cell seeding methods, and the incorporation of biologically active factors to identify the advancements and current limitations of materials and methods for the development of effective artificial bile ducts that promote tissue regeneration.

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Regeneration of the oesophageal muscle layer from oesophagus acellular matrix scaffold using adipose-derived stem cells

Cited by 21 publications

References 18 publications

Development and Characterization of a Porcine Liver Scaffold

Development and Characterization of a Porcine Liver Scaffold

Experimental investigation of esophageal reconstruction with electrospun polyurethane nanofiber and 3D printing polycaprolactone scaffolds using a rat model

Progress and Current Limitations of Materials for Artificial Bile Duct Engineering

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