Identification of a type II cystatin in Fragaria chiloensis: A proteinase inhibitor differentially regulated during achene development and in response to biotic stress-related stimuli

Aceituno-Valenzuela, Uri; Covarrubias, María Paz; Aguayo, M; Valenzuela-Riffo, Felipe; Espinoza, Analía; Gaete-Eastman, Carlos; Herrera, Raúl; Handford, Michael; Norambuena, Lorena

doi:10.1016/j.plaphy.2018.05.021

Cited by 8 publications

(4 citation statements)

References 55 publications

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“…Sequence comparison of other five type II phytocystatins which had both domains individually tested against papain and different legumains indicated conservation of the properties seen in AtCYT6: (i) an asparagine residue on the RCL of N‐ and C‐terminal domains, (ii) an R‐G‐X‐K basic motif on the LEL of the legumain‐inhibitory CTD, and a single Asp or Glu on the LEL of their NTDs (Aceituno‐Valenzuela et al., 2018 ; Christoff et al., 2016 ; Martinez et al., 2007 ) (Figure S6 ).…”

Section: Resultsmentioning

confidence: 99%

Phytocystatin 6 is a context‐dependent, tight‐binding inhibitor of Arabidopsis thaliana legumain isoform β

Santos,

Soh,

Demir

et al. 2023

The Plant Journal

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SUMMARYPlant legumains are crucial for processing seed storage proteins and are critical regulators of plant programmed cell death. Although research on legumains boosted recently, little is known about their activity regulation. In our study, we used pull‐down experiments to identify AtCYT6 as a natural inhibitor of legumain isoform β (AtLEGβ) in Arabidopsis thaliana. Biochemical analysis revealed that AtCYT6 inhibits both AtLEGβ and papain‐like cysteine proteases through two separate cystatin domains. The N‐terminal domain inhibits papain‐like proteases, while the C‐terminal domain inhibits AtLEGβ. Furthermore, we showed that AtCYT6 interacts with legumain in a substrate‐like manner, facilitated by a conserved asparagine residue in its reactive center loop. Complex formation was additionally stabilized by charged exosite interactions, contributing to pH‐dependent inhibition. Processing of AtCYT6 by AtLEGβ suggests a context‐specific regulatory mechanism with implications for plant physiology, development, and programmed cell death. These findings enhance our understanding of AtLEGβ regulation and its broader physiological significance.

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Section: Resultsmentioning

confidence: 99%

Phytocystatin 6 is a context‐dependent, tight‐binding inhibitor of Arabidopsis thaliana legumain isoform β

Santos,

Soh,

Demir

et al. 2023

The Plant Journal

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“…Most plant cysteine PIs, which specifically inhibit the activity of cysteine proteases and are found in plants, insects, and vertebrates, are part of the cystatin superfamily [32]. The cystatin from plants has been classified in a new subfamily (called phytocystatins) due to its special pattern [70]. Although cystatins have been identified in many plant species, only a few cystatins have been well characterized [35].…”

Section: Classification and Function Of Pi Genes In Tomatomentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the Protease Inhibitor Gene Families in Tomato

Fan

Yang

Yan

et al. 2019

Genes

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The protease inhibitors (PIs) in plants are involved primarily in defense against pathogens and pests and in response to abiotic stresses. However, information about the PI gene families in tomato (Solanum lycopersicum), one of the most important model plant for crop species, is limited. In this study, in silico analysis identified 55 PI genes and their conserved domains, phylogenetic relationships, and chromosome locations were characterized. According to genetic structure and evolutionary relationships, the PI gene families were divided into seven families. Genome-wide microarray transcription analysis indicated that the expression of SlPI genes can be induced by abiotic (heat, drought, and salt) and biotic (Botrytis cinerea and tomato spotted wilt virus (TSWV)) stresses. In addition, expression analysis using RNA-seq in various tissues and developmental stages revealed that some SlPI genes were highly or preferentially expressed, showing tissue-and developmental stage-specific expression profiles. The expressions of four representative SlPI genes in response to abscisic acid (ABA), salicylic acid (SA), ethylene (Eth), gibberellic acid (GA). and methyl viologen (MV) were determined. Our findings indicated that PI genes may mediate the response of tomato plants to environmental stresses to balance hormone signals. The data obtained here will improve the understanding of the potential function of PI gene and lay a foundation for tomato breeding and transgenic resistance to stresses.

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“…This could be further useful for the selection of the phenotype for separation of bioactive compounds for targeted applications. Two different compounds (a protease enzyme with molecular weight 65.8 kDa, stable at high temperatures and over a wide pH range, with specificity toward hemoglobin, respectively a cysteine protease inhibitor cystatin FchCYS1) were isolated in 2018 from Fragaria x ananassa [22], respectively Fragaria chiloensis [23], while a new ellagitannin (a galloylated derivative of agrimoniin, with molecular weight 2038) named fragariin A was isolated in 2019 by Karlińska et al from strawberry fruits (Fragaria x ananassa Duch.) [24].…”

Section: Anthocyaninsmentioning

confidence: 99%

Fragaria Genus: Chemical Composition and Biological Activities

et al. 2020

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The strawberries represent in our days one of the main fresh fruits consumed globally, inevitably leading to large amounts of by-products and wastes. Usually appreciated because of their specific flavor, the strawberries also possess biological properties, including antioxidant, antimicrobial, or anti-inflammatory effects. In spite of the wide spread of the Fragaria genus, few species represent the subject of the last decade scientific research. The main components identified in the Fragaria species are presented, as well as several biological properties, as emerging from the scientific papers published in the last decade.

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Identification of a type II cystatin in Fragaria chiloensis: A proteinase inhibitor differentially regulated during achene development and in response to biotic stress-related stimuli

Cited by 8 publications

References 55 publications

Phytocystatin 6 is a context‐dependent, tight‐binding inhibitor of Arabidopsis thaliana legumain isoform β

Phytocystatin 6 is a context‐dependent, tight‐binding inhibitor of Arabidopsis thaliana legumain isoform β

Genome-Wide Identification and Expression Analysis of the Protease Inhibitor Gene Families in Tomato

Fragaria Genus: Chemical Composition and Biological Activities

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