Insulin-Like Growth Factor-1 as a Possible Alternative to Bone Morphogenetic Protein-7 to Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells in Vitro

Reible, Bruno; Schmidmaier, Gerhard; Moghaddam, Arash; Westhauser, Fabian

doi:10.3390/ijms19061674

Cited by 65 publications

(66 citation statements)

References 54 publications

(93 reference statements)

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“…Primary cells are isolated from donor patients. These can either be osteoblasts that are isolated after digestion of cancellous bone or osteoblast precursors, such as BMSCs, that are usually isolated by density-gradient centrifugation of bone marrow (Hoellig et al, 2016;Reible et al, 2018). Since these cells originate from the anticipated field of application for BGs, they are suitable to evaluate the influence of the materials on bone related cells improving transferability of in vitro results to the "clinical situation".…”

Section: U2osmentioning

confidence: 99%

“…However, under in-vitro conditions, BMSCs need osteogenic differentiation stimuli to commit to osteogenic differentiation (Reible et al, 2017;Reible et al, 2018). BMSCs are therefore suitable to assess the impact of materials, growth factors, differentiation factors, etc.…”

Section: Bmscsmentioning

confidence: 99%

“…BMSCs are therefore suitable to assess the impact of materials, growth factors, differentiation factors, etc. on osteogenic differentiation since they will develop from an undifferentiated state in certain steps towards osteoblasts, exhibiting gene expression patterns of mature osteoblasts even under in vitro conditions (Birmingham et al, 2012;Reible et al, 2017;Reible et al, 2018;Westhauser et al, 2019). Like HOBs, BMSCs are of special interest in this setting, as they both yield high potential for following in vivo BTE projects.…”

Section: Bmscsmentioning

confidence: 99%

“…Cells without the addition of 45S5-BG served as controls. This assay is based on an ALP mediated hydrolysis of p-NPP to para-nitrophenol resulting in a color change from transparent to yellow that correlates with the enzymatic activity of ALP(Reible et al, 2017;Reible et al, 2018;Widholz et al, 2019). The nonfluorescent FDA can freely pass cell membranes and is actively converted by living cells to the greenfluorescent compound fluorescein that can be used to visualize cell morphology and to measure cell viability.…”

mentioning

confidence: 99%

“…Both, cell lines and primary cells are included (Gough, Clupper et al 2004;Huang, Jayasinghe et al 2005;Jones, Tsigkou et al 2007;Deb, Mandegaran et al 2010;Fan, Kalia et al 2014;Xynos et al, 2000;Xynos et al, 2001) BMSC Primary Bone marrow-derived mesenchymal stromal cell (BMSC), early osteoblast precursor. Usually isolated by density-gradient centrifugation of bone marrow (Reible, Schmidmaier et al 2017;Reible, Schmidmaier et al 2018). (Qazi, Hafeez et al 2017;Qazi, Berkmann et al 2018;Westhauser, Karadjian et al 2019) MG-63 Cell line Osteosarcoma cells, derived from a 13-year-old male Caucasian patient (Billiau, Edy et al 1977;Czekanska, Stoddart et al 2012).…”

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confidence: 99%

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Primary osteoblasts, osteoblast precursor cells or osteoblast‐like cell lines: Which human cell types are (most) suitable for characterizing 45S5‐bioactive glass?

Wilkesmann

Fellenberg

Nawaz

et al. 2019

J Biomedical Materials Res

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The question how bioactive glasses (BGs) influence the viability and osteogenic differentiation of human osteogenic cells has already been addressed by several studies.However, a literature review revealed great differences in the type of cells used for these experiments. Primary human osteoblasts (hOBs) represent the desired standard, but possess the limitation of patient variability and time-consuming isolation protocols. Therefore, several alternative cell types have been used including primary mesenchymal stromal cells (BMSCs) and the "osteoblast-like" cell lines MG-63, Saos-2, HOS, and U2OS. The aim of our study was the identification of the cell type most suitable for tissue engineering projects involving BGs by comparative analysis of cell viability and osteogenic differentiation in response to crystallized 45S5-BG. We observed that hOBs, BMSCs, and MG-63 cells were resistant to 45S5-BG induced cytotoxicity, while the viability of Saos-2, HOS, and U2OS cells was significantly reduced. In addition, we detected alkaline phosphatase activity, except in U2OS cells, that increased upon 45S5-BG cocultivation, demonstrating the induction of osteogenic differentiation. Our data and the fact that the donor-dependent variations can be avoided when using MG-63 cells suggest that these are a promising alternative to primary cells and remain an important cell line for future BG related studies.

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Section: U2osmentioning

confidence: 99%

Section: Bmscsmentioning

confidence: 99%

Section: Bmscsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Primary osteoblasts, osteoblast precursor cells or osteoblast‐like cell lines: Which human cell types are (most) suitable for characterizing 45S5‐bioactive glass?

Wilkesmann

Fellenberg

Nawaz

et al. 2019

J Biomedical Materials Res

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Spatiotemporal Control Strategies for Bone Formation through Tissue Engineering and Regenerative Medicine Approaches

White

Olabisi

2018

Adv Healthcare Materials

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Global increases in life expectancy drive increasing demands for bone regeneration. The gold standard for surgical bone repair is autografting, which enjoys excellent clinical outcomes; however, it possesses significant drawbacks including donor site morbidity and limited availability. Although collagen sponges delivered with bone morphogenetic protein, type 2 (BMP2) are a common alternative or supplement, they do not efficiently retain BMP2, necessitating extremely high doses to elicit bone formation. Hence, reports of BMP2 complications are rising, including cancer promotion and ectopic bone formation, the latter inducing complications such as breathing difficulties and neurologic impairments. Thus, efforts to exert spatial control over bone formation are increasing. Several tissue engineering approaches have demonstrated the potential for targeted and controlled bone formation. These approaches include biomaterial scaffolds derived from synthetic sources, e.g., calcium phosphates or polymers; natural sources, e.g., bone or seashell; and immobilized biofactors, e.g., BMP2. Although BMP2 is the only protein clinically approved for use in a surgical device, there are several proteins, small molecules, and growth factors that show promise in tissue engineering applications. This review profiles the tissue engineering advances in achieving control over the location and onset of bone formation (spatiotemporal control) toward avoiding the complications associated with BMP2.

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Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles

Westhauser

Wilkesmann

Nawaz

et al. 2020

J Biomedical Materials Res

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Mesoporous bioactive glass nanoparticles (MBGNs) have demonstrated promising properties for the local delivery of therapeutically active ions with the aim to improve their osteogenic properties. Manganese (Mn), zinc (Zn), and copper (Cu) ions have already shown promising pro‐osteogenic properties. Therefore, the concentration‐dependent impact of MBGNs (composition in mol%: 70 SiO2, 30 CaO) and MBGNs containing 5 mol% of either Mn, Zn, or Cu (composition in mol%: 70 SiO2, 25 CaO, 5 MnO/ZnO/CuO) on the viability and osteogenic differentiation of human marrow‐derived mesenchymal stromal cells (BMSCs) was assessed in this study. Mn‐doped MBGNs (5Mn‐MBGNs) showed a small “therapeutic window” with a dose‐dependent negative impact on cell viability but increasing pro‐osteogenic features alongside increasing Mn concentrations. Due to a constant release of Zn, 5Zn‐MBGNs showed good cytocompatibility and upregulated the expression of genes encoding for relevant members of the osseous extracellular matrix during the later stages of cultivation. In contrast to all other groups, BMSC viability increased with increasing concentration of Cu‐doped MBGNs (5Cu‐MBGNs). Furthermore, 5Cu‐MBGNs induced an increase in alkaline phosphatase activity. In conclusion, doping with Mn, Zn, or Cu can enhance the biological properties of MBGNs in different ways for their potential use in bone regeneration approaches.

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Insulin-Like Growth Factor-1 as a Possible Alternative to Bone Morphogenetic Protein-7 to Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells in Vitro

Cited by 65 publications

References 54 publications

Primary osteoblasts, osteoblast precursor cells or osteoblast‐like cell lines: Which human cell types are (most) suitable for characterizing 45S5‐bioactive glass?

Primary osteoblasts, osteoblast precursor cells or osteoblast‐like cell lines: Which human cell types are (most) suitable for characterizing 45S5‐bioactive glass?

Spatiotemporal Control Strategies for Bone Formation through Tissue Engineering and Regenerative Medicine Approaches

Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles

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