Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids

Aanei, Ioana L.; Glasgow, Jeff E.; Capehart, Stacy L.; Francis, Matthew B.

doi:10.1007/978-1-4939-7808-3_21

Cited by 7 publications

(5 citation statements)

References 39 publications

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“…The successful encapsulation of cargo depends on the size and surface charge of the cargo, electrostatic interactions, hydrophobicity/hydrophilicity, and other unique binding interactions that occur during nanoassembly [15][16][17]. In some cases, the reassembly is based on electrostatic interactions between the positively charged interior surfaces of the capsid proteins and a negatively charged cargo that mimics the negatively charged nucleic acids [18]. Encapsulation of positively charged payloads can be accomplished by mixing with another negatively charged molecule providing sufficient net negative charge and thereby catalyzing capsid assembly [19].…”

Section: Encapsulation and Conjugation Techniques For Vlpsmentioning

confidence: 99%

“…NV1023 is an oncolytic herpes simplex virus with preferential targeting towards tumor cells. The virus platform technology was engineered to catalyze the production of [ 18 Although CT is oftentimes used concurrently with PET, it can also be employed as a stand-alone imaging modality. Recently, gold-coated VNPs were developed using CPMV.…”

Section: Vlps In Pet and Ct Imagingmentioning

confidence: 99%

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Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Chung

Cai

Steinmetz

2020

Advanced Drug Delivery Reviews

285

241

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Viral nanoparticles (VNPs) encompass a diverse array of naturally occurring nanomaterials derived from plant viruses, bacteriophages, and mammalian viruses. The application and development of VNPs and their genomefree versions, the virus-like particles (VLPs), for nanomedicine is a rapidly growing. VLPs can encapsulate a wide range of active ingredients as well as be genetically or chemically conjugated to targeting ligands to achieve tissue specificity. VLPs are manufactured through scalable fermentation or molecular farming, and the materials are biocompatible and biodegradable. These properties have led to a wide range of applications, including cancer therapies, immunotherapies, vaccines, antimicrobial therapies, cardiovascular therapies, gene therapies, as well as imaging and theranostics. The use of VLPs as drug delivery agents is evolving, and sufficient research must continuously be undertaken to translate these therapies to the clinic. This review highlights some of the novel research efforts currently underway in the VNP drug delivery field in achieving this greater goal.

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Section: Encapsulation and Conjugation Techniques For Vlpsmentioning

confidence: 99%

Section: Vlps In Pet and Ct Imagingmentioning

confidence: 99%

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Chung

Cai

Steinmetz

2020

Advanced Drug Delivery Reviews

285

241

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“…If successfully developed, VLPs, especially from ΦIN93 (with a diameter of 130 nm), will have the capacity to be loaded with more cargo (vaccine adjuvants, imaging fluorophores) for targeted delivery to cells. The diameter of ΦIN93 is almost twice the size of thermophilic P23-77 (78 nm) and more than four times the size of mesophilic MS2 VLPs (~28 nm), which is widely used in the field to deliver cargo [110][111][112]. The sizes of P23-77 and ΦIN93 have additional benefits as platforms for vaccine design/immunization.…”

Section: Conclusion and Perspectives For The Futurementioning

confidence: 99%

Potential Applications of Thermophilic Bacteriophages in One Health

Liu

Khezerloo

Tumban

2023

IJMS

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Bacteriophages have a wide range of applications such as combating antibiotic resistance, preventing food contamination for food safety, and as biomarkers to indirectly assess the quality of water. Additionally, bacteriophage components (endolysins and coat proteins) have a lot of applications in food processing, vaccine design, and the delivery of cargo to the body. Therefore, bacteriophages/components have a multitude of applications in human, plant/veterinary, and environmental health (One Health). Despite their versatility, bacteriophage/component use is mostly limited to temperatures within 4–40 °C. This limits their applications (e.g., in food processing conditions, pasteurization, and vaccine design). Advances in thermophilic bacteriophage research have uncovered novel thermophilic endolysins (e.g., ΦGVE2 amidase and MMPphg) that can be used in food processing and in veterinary medicine. The endolysins are thermostable at temperatures > 65 °C and have broad antimicrobial activities. In addition to thermophilic endolysins, enzymes (DNA polymerase and ligases) derived from thermophages have different applications in molecular biology/biotechnology: to generate DNA libraries and develop diagnostics for human and animal pathogens. Furthermore, coat proteins from thermophages are being explored to develop virus-like particle platforms with versatile applications in human and animal health. Overall, bacteriophages, especially those that are thermophilic, have a plethora of applications in One Health.

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“…This method is simple, cargo‐friendly, and encapsulates different types of cargo inside VNPs (Li et al, 2019). Furthermore, self‐assembly‐based loading can be accomplished through electrostatic interactions of negatively charged cargo, similar to the viral genome, and the positively charged amino acids existing on the interior surface of the capsid (Aanei et al, 2018; Chung et al, 2020). Loading of positively charged cargos can also be triggered with a coat of negatively charged molecules (Chung et al, 2020; Ren et al, 2007).…”

Section: Strategies For Multifunctional Pvnps Formulationsmentioning

confidence: 99%

Multifunctional plant virus nanoparticles: An emerging strategy for therapy of cancer

Azizi

Shahgolzari

Karkan

et al. 2022

WIREs Nanomed Nanobiotechnol

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Cancer therapy requires sophisticated treatment strategies to obtain the highest success. Nanotechnology is enabling, revolutionizing, and multidisciplinary concepts to improve conventional cancer treatment modalities. Nanomaterials have a central role in this scenario, explaining why various nanomaterials are currently being developed for cancer therapy. Viral nanoparticles (VNPs) have shown promising performance in cancer therapy due to their unique features. VNPs possess morphological homogeneity, ease of functionalization, biocompatibility, biodegradability, water solubility, and high absorption efficiency that are beneficial for cancer therapy applications. In the current review paper, we highlight state‐of‐the‐art properties and potentials of plant viruses, strategies for multifunctional plant VNPs formulations, potential applications and challenges in VNPs‐based cancer therapy, and finally practical solutions to bring potential cancer therapy one step closer to real applications.This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures

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Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids

Cited by 7 publications

References 39 publications

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Potential Applications of Thermophilic Bacteriophages in One Health

Multifunctional plant virus nanoparticles: An emerging strategy for therapy of cancer

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