3D printed tissue engineered model for bone invasion of oral cancer

Abstract

Recent advances in three-dimensional printing technology have led to a rapid expansion of its applications in tissue engineering. The present study was designed to develop and characterize an in vitro multi-layered human alveolar bone, based on a 3D printed scaffold, combined with tissue engineered oral mucosal model. The objective was to incorporate oral squamous cell carcinoma (OSCC) cell line spheroids to the 3D model at different anatomical levels to represent different stages of oral cancer. Histological evaluation of the 3D tissue model revealed a tri-layered structure consisting of distinct epithelial, connective tissue, and bone layers; replicating normal oral tissue architecture. The mucosal part showed a well-differentiated stratified oral squamous epithelium similar to that of the native tissue counterpart, as demonstrated by immunohistochemistry for cytokeratin 13 and 14. Histological assessment of the cancerous models demonstrated OSCC spheroids at three depths including supra-epithelial level, sub-epithelial level, and deep in the connective tissue-bone interface. The 3D tissue engineered composite model closely simulated the native oral hard and soft tissues and has the potential to be used as a valuable in vitro model for the investigation of bone invasion of oral cancer and for the evaluation of novel diagnostic or therapeutic approaches to manage OSCC in the future.

Prognostic and predictive markers for perineural and bone invasion of oral squamous cell carcinoma

“…Recent advances in threedimensional printing technology have led to a rapid expansion of its applications in tissue engineering. The 3D tissue engineered composite model closely simulates the native oral hard and soft tissues and has the potential to be used as a valuable in vitro model for the investigation of bone invasion of oral cancer and for the evaluation of novel diagnostic or therapeutic approaches to manage OSCC in the future 72 .…”

Die Tumorstammzellnische im Kopf-Hals-Bereich – Knotenpunkt mit therapeutischem Potenzial?

“…Die Methode des 3D-Bioprintings mehrerer Biomaterialien bietet dabei in Zukunft vielleicht die Möglichkeit zur Konstruktion des Mikromilieus (Nische). Diese Technologien bieten durch eine gezielte 3-dimensionale Anordnung unterschiedlicher Matrixbestandteile die Möglichkeit, Mechanismen der Tumorzellinvasion und -metastasierung genauer zu analysieren und potenzielle Therapiemechanismen aufzudecken [42][43][44]. Die 3-dimensionale Darstellung und die Möglichkeit, unterschiedliche Zellzusammensetzungen herzustellen, wird durch das 3D-Bioprinting stark vereinfacht.…”

“…Die Chance, die Interaktionen von Tumor(stamm)zellen mit Zellen der Mikroumgebung, insbesondere mit Immunzellen desselben Patienten, zu untersuchen, wird helfen, unser Verständnis für die neue Therapiesäule der Immuntherapie zu verbessern [43]. Aktuelle Studien zeigen, dass diese Art Tumormodell die Entwicklung von Tumorimpfstoffen ermöglichen kann.…”

3D Printing—Encompassing the Facets of Dentistry

“…A multitude of materials is used to fabricate cell-laden 3D-printed scaffolds, for example, chitosan (Intini et al, 2018 ), calcium silicate complex (Chen et al, 2018 ), and controlled-release polymeric materials with bioactive agents (Rahman et al, 2018 ). 3D printing has found its application in generating optimal volumes of human bone and skin grafts in vitro (Lee et al, 2014 ; Almela et al, 2018 ). This is gaining immense potential to replace the current strategies of procuring autografts which are associated with donor site morbidity and loss in structure (Chiarello et al, 2013 ).…”