Detoxification of<i>Jatropha curcas</i>seed cake by a new soil-borne<i>Enterobacter cloacae</i>strain

Zhao, Yanfang; Wang, Haifeng; Liu, J.X.

doi:10.1111/lam.13012

Cited by 7 publications

(3 citation statements)

References 29 publications

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“…Other bacteria and fungi have previously been reported to be capable of phorbol ester detoxification, including Enterobacter spp., Bacillus spp., and Pleurotus spp. [ 28 , 29 , 30 ]. It would be useful to look towards the microorganisms that have been found to successfully detoxify phorbol esters and examine whether they are similarly able to detoxify simplexin and produce any of the targeted H 2 SO 4 hydrolysis products 8 , 9 , 10 , and 11 , although the relative stability of the simplexin orthoester [ 17 ] could be confounding.…”

Section: Resultsmentioning

confidence: 99%

Identification of Acid Hydrolysis Metabolites of the Pimelea Toxin Simplexin for Targeted UPLC-MS/MS Analysis

Loh,

Hungerford,

Ouwerkerk

et al. 2023

Toxins

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Pimelea poisoning of cattle is a unique Australian toxic condition caused by the daphnane orthoester simplexin present in native Pimelea pasture plants. Rumen microorganisms have been proposed to metabolise simplexin by enzymatic reactions, likely at the orthoester and epoxide moieties of simplexin, but a metabolic pathway has not been confirmed. This study aimed to investigate this metabolic pathway through the analysis of putative simplexin metabolites. Purified simplexin was hydrolysed with aqueous hydrochloric acid and sulfuric acid to produce target metabolites for UPLC-MS/MS analysis of fermentation fluid samples, bacterial isolate samples, and other biological samples. UPLC-MS/MS analysis identified predicted hydrolysed products from both acid hydrolysis procedures with MS breakdown of these putative products sharing high-resolution accurate mass (HRAM) fragmentation ions with simplexin. However, targeted UPLC-MS/MS analysis of the biological samples failed to detect the H2SO4 degradation products, suggesting that the rumen microorganisms were unable to produce similar simplexin degradation products at detectable levels, or that metabolites, once formed, were further metabolised. Overall, in vitro acid hydrolysis was able to hydrolyse simplexin at the orthoester and epoxide functionalities, but targeted UPLC-MS/MS analysis of biological samples did not detect any of the identified simplexin hydrolysis products.

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Section: Resultsmentioning

confidence: 99%

Identification of Acid Hydrolysis Metabolites of the Pimelea Toxin Simplexin for Targeted UPLC-MS/MS Analysis

Loh,

Hungerford,

Ouwerkerk

et al. 2023

Toxins

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“…Exemplary strains and their phorbol ester degradation efficiency are listed as follows: Ent. cloacae Z11 (51.6%) (Zhao et al, 2018), Trichoderma harzianum JQ350879.1 (99.7%) (Najjar et al, 2014), Paecilomyces sinensis JQ350881.1 (99%) (Najjar et al, 2014), Cladosporium cladosporioides JQ517491.1 (96.4%) (Najjar et al, 2014), Cunninghamella echinulata CJS‐90 (75%) (Sharath et al, 2014), Aspergillus versicolor CJS‐98 (81.93%) (Veerabhadrappa et al, 2014) and Ps. aeruginosa PseA (100%) (Joshi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Rapid detoxification of Jatropha curcas cake by fermentation with a combination of three microbial strains and characterization of their metabolic profiles

Zhang

Chang

Wen

et al. 2022

Journal of Applied Microbiology

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Aim Our previous study reported a strain that can detoxify Jatropha curcas L. cake (JCC), but the detoxification duration is long. This study intends to explore the efficient detoxification of JCC through multi‐strain collaborative fermentation to accelerate the detoxification process. Methods and Results Mucor circinelloides SCYA25 strain that we previously reported can effectively degrade the toxicity of JCC, and the newly screened Bacillus megaterium SCYA10 and Geotrichum candidum SCYA23 strains were used to detoxify JCC. Different solid‐state‐fermentation (SSF) parameters were optimized by single‐factor tests and response surface methodology. A detoxification rate established by zebrafish toxicity of JCC at 96% was achieved under the following optimized conditions: the combination ratio of B. megaterium SCYA10, G. candidum SCYA23 and M. circinelloides SCYA25 at 2:3:1, a total injection amount of 15.25%, a feed to water ratio of 1:0.68, a fermentation temperature of 30.3°C and fermentation duration of 21.5 days. The protein content of fermented JCC (FJCC) increased, while the concentrations of ether extract, crude fibre and toxins were all degraded considerably. Metabolomics analysis revealed that the fermentation increased the contents of neurotransmitter receptor modulator, emulsifier, aromatic substances and insecticidal compounds, as well as decreasing the contents of oxidative stress and neurotoxic substances. A rat feeding trial showed that the growth performance of the rats provided with the FJCC diet was similar to that of the corn‐soybean meal group, and no lesions in the liver and kidney were observed. Conclusion The co‐bio‐fermentation process can effectively detoxify JCC and improve its nutritional value, which means it could be served as a protein feed in animal husbandry. Significance The combination of three microbial strains can detoxify JCC in a safe and effective manner to provide a great potential alternative to soybean meal. The research also suggests that metabonomics and bioinformatics are useful tools for revealing the bio‐detoxification mechanism.

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“…Zhao et al . (2018) found that a soil‐borne Enterobacter cloacae can degrade the content of phorbol esters in JCC after 5 day’s fermentation. Aspergillus versicolor was reported to be able to degrade 81·93% of the phorbol esters in JCC after 96 h SSF (Veerabhadrappa et al .…”

Section: Introductionmentioning

confidence: 99%

Bio‐detoxification of Jatropha curcas L. cake by a soil‐borne Mucor circinelloides strain using a zebrafish survival model and solid‐state fermentation

Zhang

Chang

Tang

et al. 2020

J. Appl. Microbiol.

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Aims The aims of the study were to (i) improve the evaluation criteria of detoxifying Jatropha curcas L. cake (JCC), (ii) isolate and characterize a JCC tolerant strain, (iii) explore its JCC detoxifying potential. Methods and Results The zebrafish was employed as a survival model to screen the strains capable of detoxifying JCC. A strain identified as Mucor circinelloides SCYA25, which is highly capable of degrading all toxic components, was isolated from soil. Different solid‐state fermentation parameters were optimized by response surface methodology. The optimal values for inoculation amount, moisture content, temperature, and time were found to be 18% (1·8 × 106 spores g−1 cake), 66%, 26, and 36 days, respectively, to achieve maximum detoxification of the JCC (92%). Under optimal fermentation conditions, the protein content of JCC was increased, while the concentrations of ether extract, crude fiber, toxins, and anti‐nutritional substances were all degraded considerably (P < 0·05). Scanning electron microscopy and Fourier transform infrared spectrometer analysis revealed that the fermentation process could disrupt the surface structure and improve the ratio of α‐helix to β‐folding in the JCC protein, which may improve the digestibility when the detoxified JCC is used as a feedstuff. Conclusions Our results indicate that M. circinelloides SCYA25 is able to detoxify JCC and improve its nutritional profile, which is beneficial to the safe utilization of JCC as a protein feedstuff. Significance and Impact of the Study The newly identified M. circinelloides SCYA25 detoxified JCC in a safe manner to provide a potential alternative to soybean meal for the feed industry. These results also provide a new perspective and method for the toxicity evaluation and utilization of JCC and similar toxic agricultural by‐products.

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Detoxification ofJatropha curcasseed cake by a new soil-borneEnterobacter cloacaestrain

Cited by 7 publications

References 29 publications

Identification of Acid Hydrolysis Metabolites of the Pimelea Toxin Simplexin for Targeted UPLC-MS/MS Analysis

Identification of Acid Hydrolysis Metabolites of the Pimelea Toxin Simplexin for Targeted UPLC-MS/MS Analysis

Rapid detoxification of Jatropha curcas cake by fermentation with a combination of three microbial strains and characterization of their metabolic profiles

Bio‐detoxification of Jatropha curcas L. cake by a soil‐borne Mucor circinelloides strain using a zebrafish survival model and solid‐state fermentation

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