Novel murine model for delayed wound healing using a biological wound dressing with Pseudomonas aeruginosa biofilms

Brandenburg, Kenneth S.; Calderon, Diego F.; Kierski, Patricia R.; Czuprynski, Charles J.; McAnulty, Jonathan F.

doi:10.1016/j.micpath.2018.05.043

Cited by 21 publications

(20 citation statements)

References 17 publications

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“…Emerging evidence suggests the wound microbiome and the formation of bacterial biofilms may contribute to delayed wound healing (45,(133)(134)(135)(136)(137)(138). However, the causal relationship between microbiome composition, biofilm formation, dysregulated innate immune activation and persistent inflammation in chronic wounds remains poorly understood (12,139).…”

Section: Role Of Bacteria In Driving Innate Immune Activation and Inflammation In Chronic Woundsmentioning

confidence: 99%

Biofilm-Innate Immune Interface: Contribution to Chronic Wound Formation

et al. 2021

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Delayed wound healing can cause significant issues for immobile and ageing individuals as well as those living with co-morbid conditions such as diabetes, cardiovascular disease, and cancer. These delays increase a patient’s risk for infection and, in severe cases, can result in the formation of chronic, non-healing ulcers (e.g., diabetic foot ulcers, surgical site infections, pressure ulcers and venous leg ulcers). Chronic wounds are very difficult and expensive to treat and there is an urgent need to develop more effective therapeutics that restore healing processes. Sustained innate immune activation and inflammation are common features observed across most chronic wound types. However, the factors driving this activation remain incompletely understood. Emerging evidence suggests that the composition and structure of the wound microbiome may play a central role in driving this dysregulated activation but the cellular and molecular mechanisms underlying these processes require further investigation. In this review, we will discuss the current literature on: 1) how bacterial populations and biofilms contribute to chronic wound formation, 2) the role of bacteria and biofilms in driving dysfunctional innate immune responses in chronic wounds, and 3) therapeutics currently available (or underdevelopment) that target bacteria-innate immune interactions to improve healing. We will also discuss potential issues in studying the complexity of immune-biofilm interactions in chronic wounds and explore future areas of investigation for the field.

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Section: Role Of Bacteria In Driving Innate Immune Activation and Inflammation In Chronic Woundsmentioning

confidence: 99%

Biofilm-Innate Immune Interface: Contribution to Chronic Wound Formation

et al. 2021

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“…Finally, the section samples were stained with hematoxylin and eosin (H & E). The section samples were imaged using a digital slide scanner (MoticEasyScan, Motic, Xiamen, China) for histologic analysis and scored using the International Harmonization of Nomenclature and Diagnostic Criteria (INHAND) protocol [ 23 , 24 ]. The re-epithelization was graded as 0 for absence, 1 for scant presence, 2 for moderate presence and 3 for remarkable presence, while the orientation of collagen fibers and fibroblasts was graded as 0 for absence, 1 for vertical arrangement, 2 for mixed arrangement and 3 for horizontal arrangement.…”

Section: Methodsmentioning

confidence: 99%

Micropatterned Fibrous Scaffold Produced by Using Template-Assisted Electrospinning Technique for Wound Healing Application

Norzain

Lin

et al. 2021

Polymers

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This paper describes the fabrication of a structural scaffold consisting of both randomly oriented nanofibers and triangular prism patterns on the scaffold surface using a combination technique of electrospinning and collector templates. The polycaprolactone (PCL) nanofibers were electrospun over a triangular prism pattern mold, which acted as a template. The deposited scaffold was removed from the template to produce a standalone structural scaffold of three-dimensional micropatterned nanofibers. The fabricated structural scaffold was compared with flat randomly oriented nanofibers based on in vitro and in vivo studies. The in vitro study indicated that the structural scaffold demonstrated higher fibroblast cell proliferation, cell elongation with a 13.48 ± 2.73 aspect ratio and 70% fibroblast cell orientation compared with flat random nanofibers. Among the treatment groups, the structural scaffold escalated the wound closure to 92.17% on day 14. Histological staining of the healed wound area demonstrated that the structural scaffold exhibited advanced epithelization of the epidermal layer accompanied by mild inflammation. The proliferated fibroblast cells and collagen fibers in the structural scaffold appeared denser and arranged more horizontally. These results determined the potential of micropatterned scaffolds for stimulating cell behavior and their application for wound healing.

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“…Another potential mechanism for P. aeruginosa biofilm resistance to neutrophils is the rapid necrosis induced by the production of rhamnolipids [23]. Additionally, significant delay in wound healing, re-epithelization and collagen deposition have been reported without significant difference in PMN infiltration or granulation tissue [6]. The ultimate result is neutrophil aggregation near the biofilm, with oxidative burst products accumulating and causing neutrophil death, while individuals within the biofilm leave to create new colonies away from the initial site [24,25].…”

Section: Wound Healing Inhibitionmentioning

confidence: 99%

“…Biofilms have been reported to be present in 60% [4] to 80% [5] of chronic wounds, and a recent meta-analysis confirms their presence in 78.2% of chronic wounds [2]. Therefore, biofilms have been categorized as an important factor in most chronic non-healing skin wounds [6].…”

Section: Introductionmentioning

confidence: 99%

“…The relationship between biofilm and host will depend on the location and the bacterial composition of the biofilm; for instance, in the gastrointestinal mucosa, biofilm has a commensal behavior, while in wounds or respiratory tract mucosa, a pathogenic behavior. This difference is thought to be due to the host's capacity to coexist or eradicate biofilm [6,12,17,18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Impact of Biofilm Formation on Wound Healing

Mendoza¹,

Hsieh²,

Galiano³

2019

Wound Healing - Current Perspectives

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Chronic wounds represent an important challenge for wound care and are universally colonized by bacteria. These bacteria can form biofilm as a survival mechanism that confers the ability to resist environmental stressors and antimicrobials due to a variety of reasons, including low metabolic activity. Additionally, the exopolymeric substance (EPS) contained in biofilm acts as a mechanical barrier to immune system cells, leading to collateral damage in the surrounding tissue as well as chronic inflammation, which eventually will delay healing of the wound. This chapter will discuss current knowledge on biofilm formation, its presence in acute and chronic wounds, how biofilm affects antibiotic resistance and tolerance, as well as the wound healing process. We will also discuss proposed methods to eliminate biofilm and improve wound healing despite its presence, including basic science and clinical studies regarding these matters.

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Novel murine model for delayed wound healing using a biological wound dressing with Pseudomonas aeruginosa biofilms

Cited by 21 publications

References 17 publications

Biofilm-Innate Immune Interface: Contribution to Chronic Wound Formation

Biofilm-Innate Immune Interface: Contribution to Chronic Wound Formation

Micropatterned Fibrous Scaffold Produced by Using Template-Assisted Electrospinning Technique for Wound Healing Application

The Impact of Biofilm Formation on Wound Healing

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