Species of the Metarhizium anisopliae complex with diverse ecological niches display different susceptibilities to antifungal agents

Brancini, Guilherme Thomaz Pereira; Tonani, Ludmilla; Rangel, Drauzio E.N.; Roberts, Donald W.; Braga, Gilberto Úbida Leite

doi:10.1016/j.funbio.2017.12.004

Cited by 11 publications

(5 citation statements)

References 62 publications

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“…DNA sequencing and phylogenic analysis tentatively positioned these isolates in to M. anisopliae species (Fig. 4), in which the topology was similar to that described by other authors (Brancini et al 2018). Relationship check was assured by BLAST search method and all of the isolates that have 99% and above similarity with sequence of M. anisopliae species quarried from GenBank.…”

Section: Resultssupporting

confidence: 69%

Morphological, molecular, and pathogenicity characteristics of the native isolates of Metarhizium anisopliae against the tomato leafminer, Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae) in Ethiopia

Ayele

Muleta

Venegas

et al. 2020

Egypt J Biol Pest Control

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The South American tomato leafminer, Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae), is a newly introduced and a major threat of tomato production in Ethiopia. Laboratory bioassay was conducted to evaluate locally isolated Metarhizium anisopliae (Metsch.) (Sorokin), against larvae of T. absoluta. Twenty-five Metarhizium isolates were isolated from different soil types, using the great wax moth, Galleria mellonella L. baiting method. From these, 13 isolates were prescreened by biological efficiency index model and tested against 2nd and 3rd larval instars of T. absoluta at the concentration of 107 spores ml−1. Sterile water plus Tween 80 (0.1% v/v) was used as a control. Greenhouse reared larvae of T. absoluta were used as experimental organisms. All tested Metarhizium formulations were pathogenic to T. absoluta in all conducted bioassays. Three isolates, AAUM78, AAUM39, and AAUM76, were the most effective and scored 88, 90, and 95% and 90, 93.3, and 95.7% mortality against 2nd and 3rd larval instars of T. absoluta, respectively. The lowest (48.5 and 50%) mortality rate was recorded by isolate AAUM30 against 2nd and 3rd larval instars, respectively, in 7 days post inoculation. However, all isolates showed significant statistical differences with F (24, 50) = 6.825, p < 0.001 and F (23, 15) = 3.97, p < 0.001 of mortality to 2nd and 3rd larval instars, respectively. Molecular analysis categorized these selected isolates under species of M. anisopliae and grouped into 2 different groups. AAUM78 and AAUM39 were recorded LT50 values of 3.93 and 3.5 days at the lowest (104 spore ml−1) concentration and LC50 values of 1.2 × 103 and 2.9 × 103 spore ml−1, respectively. Although AAUM78 and AAUM39 were virulent to the target pest, further field evaluation is required to determine their potential efficacy for T. absoluta control.

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Section: Resultssupporting

confidence: 69%

Morphological, molecular, and pathogenicity characteristics of the native isolates of Metarhizium anisopliae against the tomato leafminer, Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae) in Ethiopia

Ayele

Muleta

Venegas

et al. 2020

Egypt J Biol Pest Control

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“…There is the ultimate hope in agricultural research to optimize the virulence of different M. anisopliae complexes by targeting each species to the agricultural environment where it may be most effective. 1 For the ophthalmologist, the large spectrum of M. anisopliae may be associated with a range of clinical features and keratitis severity.…”

Section: Discussionmentioning

confidence: 99%

“…M etarhizium robertsii is part of the Metarhizium anisopliae complex, a group of fungi that can infect insects and are found globally in soil. 1 The fungi conidia bind to the insect cuticle, and it can infect a wide range of insects, including locusts, termites, cockroaches, weevils, fruit flies, and mosquitoes. 2 Virulence is mediated by proteolytic enzymes, chymotrypsin-like proteinases, and subtilisin-like proteinases.…”

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confidence: 99%

Insect Fungus Metarhizium robertsii Is a Rare Causative Agent of Keratitis

Palazzolo

Kowalski

Nayyar

et al. 2021

Cornea

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This study aimed to describe a case of keratitis secondary to Metarhizium robertsii, a soil-dwelling fungus with typical insect pathogenicity.Methods: A Case report.Results: A 58-year-old man with a ocular history of soft contact lens overwear, poor contact lens hygiene, and ocular exposure to ground well water supplying his house was referred to our university practice for a central corneal ulcer with 40% thinning. Same-day rapid microscopic detection with Giemsa stain identified the presence of many hyphae elements, and he was ultimately diagnosed with M. robertsii keratitis. The patient's course involved severe and progressive corneal thinning that stabilized over a 3-month course of antifungal therapy.Conclusions: Metarhizium robertsii seems more aggressive than other forms of fungal keratitis; therefore, this infection may be watched more closely with the risk for quick progressive corneal thinning, even while on antifungal management.

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“…Experiments with testing different fungal species to UV stress may clarify this hypothesis. Also, it seems reasonable to check whether a brief exposure to the monochromatic light may affect the differential susceptibility of microscopic fungi to antifungal agents, similarly to what has been done using Metarhizium species (Brancini et al 2018b), as this characteristic can be an important species-specific trait in different ecological niches.…”

Section: Effects Of Monochromatic Light On Soil Fungimentioning

confidence: 99%

Long-lasting eﬀects of red and blue light exposure on the growth of soil fungi

Vinnikova

2020

Acta Biol Szeged

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The experimental assessment of inter-species difference in long lasting effects produced in fungi by a brief exposure to the monochromatic light was performed. 24-h cultures grown from 1 mm mycelium fragments of Alternaria alternata, Aspergillus clavatus, Fusarium fujikuroi, Penicillium citrinum and Trichoderma viride were exposed for 30 min to blue light (BL, 450 nm) or red light (RL, 660 nm) and cultured for the next 10 days. Radial growth rate, conidial yield and germination, contents of proteins and phenolsand fungal antibacterial activity were estimated. BL- or RL-exposure did not essentially affect the ﬁnal size of colonies of A. clavatus but delayed the growth of P. citrinum and stimulated it in A. alternata and F. fujikuroi; these changes were more profound after BL, than after RL. In T. viride the BL exposure led to a remarkable delay of growth, whereas the RL signiﬁcantly increased the growth rate. Photo-induced changes in the conidial yield, conidial germination, contents of proteins and phenols also were dependent on the light wavelength and showed strong inter-species heterogeneity. Fungal antibacterial activity in exposed cultures was similar to the unexposed control. The observed effects are indicative targets for future research of possible molecular regulatory mechanisms underlying the photobiology in different fungal taxons.

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Species of the Metarhizium anisopliae complex with diverse ecological niches display different susceptibilities to antifungal agents

Cited by 11 publications

References 62 publications

Morphological, molecular, and pathogenicity characteristics of the native isolates of Metarhizium anisopliae against the tomato leafminer, Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae) in Ethiopia

Morphological, molecular, and pathogenicity characteristics of the native isolates of Metarhizium anisopliae against the tomato leafminer, Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae) in Ethiopia

Insect Fungus Metarhizium robertsii Is a Rare Causative Agent of Keratitis

Long-lasting eﬀects of red and blue light exposure on the growth of soil fungi

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