Licorice root extract and magnesium isoglycyrrhizinate protect against triptolide-induced hepatotoxicity <i>via</i> up-regulation of the Nrf2 pathway

Tan, Qin-You; Hu, Qian; Zhu, Shengnan; Jia, Lulu; Xiao, Juan; Su, Huazhen; Huang, Shaoyuan; Zhang, Jing; Jin, Junfei

doi:10.1080/10717544.2018.1472676

Cited by 37 publications

(22 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, due to the extremely high toxicity of triptolide to kidney, liver and gastrointestinal tract, its clinical application is greatly limited. (Liu et al., 2010; Tan et al., 2018). Moreover, existing topical preparations have poor transdermal properties owing to their structural and physicochemical characteristics.…”

Section: Introductionmentioning

confidence: 99%

Cubic and hexagonal liquid crystals as drug carriers for the transdermal delivery of triptolide

et al. 2019

View full text Add to dashboard Cite

The purpose of this study was to develop and evaluate triptolide-loaded cubic and hexagonal liquid crystals for transdermal drug delivery systems (TDDSs). We prepared and characterized triptolide-loaded lyotropic liquid crystals and evaluated for their percutaneous permeation properties in vitro and in vivo . We then used the adjuvant arthritic rat model and HaCaT cells to analyze the pharmacodynamics and conduct cell-stimulating studies of these liquid crystals. The optimized preparations were identified as cubic and hexagonal phase structures, respectively. Moreover, the in vitro percutaneous penetration studies demonstrated that compared to the homemade triptolide gel, cubic and hexagonal liquid crystals could significantly increase the percutaneous cumulative penetration of drugs within 48 h. Besides, the results of skin-blood synchronous microdialysis showed that the triptolide concentration in skin was higher than that in blood, and the cubic and hexagonal liquid crystals significantly increased the bioavailability of triptolide. Triptolide-loaded cubic and hexagonal liquid crystals presented excellent anti-arthritic effects, alleviating paw swelling and inhibiting inflammation by downregulating the levels of TNF-α and IL-1β. In vitro cell-stimulating studies displayed that triptolide-loaded cubic and hexagonal liquid crystals exhibited no obvious toxicity, which exhibited that triptolide-loaded cubic and hexagonal liquid crystals were remarkable biocompatibility. Collectively, triptolide-loaded cubic and hexagonal liquid crystals represented a promising candidate for rheumatoid arthritis therapy.

show abstract

Section: Introductionmentioning

confidence: 99%

Cubic and hexagonal liquid crystals as drug carriers for the transdermal delivery of triptolide

et al. 2019

View full text Add to dashboard Cite

show abstract

“… 42 MI preserves against triptolide-produced hepatotoxicity through activating the Nrf2 signaling. 43 MI relieves fructose-mediated apoptosis of podocyte by down-regulation of miR-193a to enhance WT1. 44 However, the investigation of MI in cancer progression is limited.…”

Section: Discussionmentioning

confidence: 99%

Magnesium Isoglycyrrhizinate Induces an Inhibitory Effect on Progression and Epithelial–Mesenchymal Transition of Laryngeal Cancer via the NF-κB/Twist Signaling

Zhang¹,

Zhao²,

Xing³

et al. 2020

DDDT

View full text Add to dashboard Cite

Background Magnesium isoglycyrrhizinate (MI) was extracted from roots of the plant Glycyrrhiza glabra, which displays multiple pharmacological activities such as anti-inflammation, anti-apoptosis, and anti-tumor. Here, we aimed to investigate the effect of MI on the progression and epithelial–mesenchymal transition (EMT) of laryngeal cancer. Methods Forty laryngeal cancer clinical samples were used. The role of MI in the proliferation of laryngeal cancer cells was assessed by MTT assay, Edu assay and colony formation assay. The function of MI in the migration and invasion of laryngeal cancer cells was tested by transwell assays. The effect of MI on apoptosis of laryngeal cancer cells was determined by cell apoptosis assay. The impact of MI on tumor growth in vivo was analyzed by tumorigenicity analysis using Balb/c nude mice. qPCR and Western blot analysis were performed to measure the expression levels of gene and protein, respectively. Results We identified that EMT-related transcription factor Twist was significantly elevated in the laryngeal cancer tissues. The expression of Twist was also enhanced in the human laryngeal carcinoma HEP-2 cells compared with that in the primary laryngeal epithelial cells. The high expression of Twist was remarkably correlated with poor overall survival of patients with laryngeal cancer. Meanwhile, our data revealed that MI reduced cell proliferation, migration and invasion and enhanced apoptosis of laryngeal cancer cells in vitro. Moreover, MI decreased transcriptional activation and the expression levels of NF-κB and Twist, and alleviated EMT in vitro and in vivo. MI remarkably inhibited tumor growth and EMT of laryngeal cancer cells in vivo. Conclusion MI restrains the progression of laryngeal cancer and induces an inhibitory effect on EMT in laryngeal cancer by modulating the NF-κB/Twist signaling. Our finding provides new insights into the mechanism by which MI inhibits laryngeal carcinoma development, enriching the understanding of the anti-tumor function of MI.

show abstract

“…The name derives from the Greek word 'glykosrhiza' or sweet root. GLR is commonly isolated from Glycyrrhizae Radix in the form of a monoammonium salt (C 42 H 61 O 16 _NH 4 ), but occasionally studies are performed with the magnesium salt of 18β-glycyrrhizic acid (magnesium isoglycyrrhizinate) (Cao et al, 2019;Tan et al, 2018;Zou et al, 2018). Specific methods have been developed to monitor the extraction process and for GLR dosages in biological media (Wang, Shan, & Lü, 2020;Yu et al, 2017;Zeng et al, 2020).…”

Section: Glycyrrhizin (Glr) a Natural Productmentioning

confidence: 99%

Glycyrrhizin: An alternative drug for the treatment of COVID-19 infection and the associated respiratory syndrome?

Bailly

Vergoten

2020

Pharmacology & Therapeutics

245

205

View full text Add to dashboard Cite

Safe and efficient drugs to combat the current COVID-19 pandemic are urgently needed. In this context, we have analyzed the anti-coronavirus potential of the natural product glycyrrhizic acid (GLR), a drug used to treat liver diseases (including viral hepatitis) and specific cutaneous inflammation (such as atopic dermatitis) in some countries. The properties of GLR and its primary active metabolite glycyrrhetinic acid are presented and discussed. GLR has shown activities against different viruses, including SARS-associated Human and animal coronaviruses. GLR is a non-hemolytic saponin and a potent immuno-active anti-inflammatory agent which displays both cytoplasmic and membrane effects. At the membrane level, GLR induces cholesterol-dependent disorganization of lipid rafts which are important for the entry of coronavirus into cells. At the intracellular and circulating levels, GLR can trap the high mobility group box 1 protein and thus blocks the alarmin functions of HMGB1. We used molecular docking to characterize further and discuss both the cholesterol-and HMG boxbinding functions of GLR. The membrane and cytoplasmic effects of GLR, coupled with its long-established medical use as a relatively safe drug, make GLR a good candidate to be tested against the SARS-CoV-2 coronavirus, alone and in combination with other drugs. The rational supporting combinations with (hydroxy)chloroquine and tenofovir (two drugs active against SARS-CoV-2) is also discussed. Based on this analysis, we conclude that GLR should be further considered and rapidly evaluated for the treatment of patients with COVID-19.

show abstract

Licorice root extract and magnesium isoglycyrrhizinate protect against triptolide-induced hepatotoxicity via up-regulation of the Nrf2 pathway

Cited by 37 publications

References 34 publications

Cubic and hexagonal liquid crystals as drug carriers for the transdermal delivery of triptolide

Cubic and hexagonal liquid crystals as drug carriers for the transdermal delivery of triptolide

Magnesium Isoglycyrrhizinate Induces an Inhibitory Effect on Progression and Epithelial–Mesenchymal Transition of Laryngeal Cancer via the NF-κB/Twist Signaling

Glycyrrhizin: An alternative drug for the treatment of COVID-19 infection and the associated respiratory syndrome?

Contact Info

Product

Resources

About