Chondroitin sulfate from sturgeon bone ameliorates pain of osteoarthritis induced by monosodium iodoacetate in rats

Sun, Yingjiao; Zhang, Guozhuan; Liu, Qian; Li, Xudong; Wang, Lei; Wang, Jianfeng; Liang, Li‐Jung

doi:10.1016/j.ijbiomac.2018.05.124

Cited by 27 publications

(9 citation statements)

References 28 publications

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“…In recent years, the rest of processed fish such as cartilage has drawn considerable attention in tissue engineering applications. Several studies have been focused on GAG extraction from cartilage including chondroitin sulfate, hyaluronic acid and dermatan sulfate 24‐29 while ECM‐ derived scaffolds from fish cartilage are not reported yet.…”

Section: Introductionmentioning

confidence: 99%

Fish cartilage: A promising source of biomaterial for biological scaffold fabrication in cartilage tissue engineering

Khajavi

Hajimoradloo

Zandi

et al. 2021

J Biomedical Materials Res

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Here, engineered cartilage‐like scaffold using an extracellular matrix (ECM) from sturgeon fish cartilage provided a chondroinductive environment to stimulate cartilaginous matrix synthesis in human adipose stem cells (hASCs). Three dimensional porous and degradable fish cartilage ECM‐derived scaffold (FCS) was produced using a protocol containing chemical decellularization, enzymatic solubilization, freeze‐drying and EDC‐crosslinking treatments and the effect of different ECM concentrations (10, 20, 30, and 40 mg/ml) on prepared scaffolds was investigated through physical, mechanical and biological analysis. The histological and scanning electron microscopy analysis revealed the elimination of the cell fragments and a 3‐D interconnected porous structure, respectively. Cell viability assay displayed no cytotoxic effects. The prepared porous constructs of fish cartilage ECM were seeded with hASCs for 21 days and compared to collagen (Col) and collagen‐10% hyaluronic acid (Col‐HA) scaffolds. Cell culture results evidenced that the fabricated scaffolds could provide a proper 3‐D structure to support the adhesion, proliferation and chondrogenic differentiation of hASCs considering the synthesis of specific proteins of cartilage, collagen type II (Col II) and aggrecan (ACAN). Based on the results of the present study, it can be concluded that the porous scaffold derived from fish cartilage ECM possesses an excellent potential for cartilage tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Fish cartilage: A promising source of biomaterial for biological scaffold fabrication in cartilage tissue engineering

Khajavi

Hajimoradloo

Zandi

et al. 2021

J Biomedical Materials Res

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“…The sturgeon endoskeleton is only composed of white cartilage, suggesting that cartilage tissues of sturgeon may be able to maintain chondrocytic phenotype of chondrocytes and inhibit the calcification of cartilage (Viegas, Conceição, Fazenda, Simes, & Cancela, 2010). And chondroitin sulfate isolated from the sturgeon cartilage ameliorates pain induced osteoarthritis and suppresses the transcription of metalloproteinase genes that cause degradation of cartilage ECM (Sun et al., 2018). Together, it is hypothesized that the cartilage ECM of sturgeon may inhibit chondrocyte hypertrophy and maintain the chondrocytic phenotype of chondrocyte.…”

Section: Introductionmentioning

confidence: 99%

Decellularized sturgeon cartilage extracellular matrix scaffold inhibits chondrocyte hypertrophy in vitro and in vivo

Chen

Dai

et al. 2021

J Tissue Eng Regen Med

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Since chondrocyte hypertrophy greatly limits the efficiency of cartilage defects repairing via cartilage tissue engineering (CTE), it is critical to develop a functional CTE scaffold able to inhibit chondrocyte hypertrophy during this period of cartilage regeneration. In this study, we tested the applicability of using decellularized sturgeon cartilage ECM (dSCECM) scaffold to cease chondrocyte hypertrophy during cartilage damage repair. The dSCECM scaffolds with interconnected porous structure and pore size of 114.1 ± 20.9 μm were successfully prepared with freeze‐dry method. Chondrocytes displayed a round shape and aggregated to form cellular spheroids within dSCECM scaffolds, which is similar to their chondrocytic phenotype within cartilage in vivo. Higher transcriptional level of chondrogenic related genes and integrin related genes was observed in chondrocytes incubated with dSCECM scaffolds instead of type I collagen (COL I) scaffolds, which were used as the control due to their widely usage in CTE and clinic applications. Furthermore, it confirmed that, compared with COL I scaffolds, dSCECM scaffolds significantly reduced the transcription of chondrocyte hypertrophy related genes in chondrocytes following the hypertrophic induction treatment. To test the ability of dSCECM scaffold to inhibit chondrocytes hypertrophy in vivo, chondrocytes with dSCECM scaffolds and COL I scaffolds were cultured with hypertrophic media and were implanted into nude mice respectively. Following 4 weeks implantation, interestingly, only the specimens derived from COL I scaffolds displayed consequences of chondrocyte hypertrophy like calcification deposition, demonstrating that chondrocyte hypertrophy is ceased by the dSCECM scaffold following hypertrophic induction. It suggests that the dSCECM scaffold can be potentially applied in clinical treating cartilage defects via the CTE approach to avoid the risk of chondrocyte hypertrophy.

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“…Chondroitin sulfate (CS), a glycosaminoglycan that is widely extracted from animal and fish cartilage, is an essential component of the extracellular matrix ( 148 ). A study indicated that carp chondroitin sulfate increased MMP and inhibited the levels of Caspase-3 and Caspase-9 by reducing mitochondrial fission, which decreased chondrocyte apoptosis ( 33 ).…”

Section: Exogenous Drugs To Optimize Mitochondria In Oamentioning

confidence: 99%

Mitochondria: Potential Targets for Osteoarthritis

Mao

Wang

et al. 2020

Front. Med.

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Osteoarthritis (OA) is a common and disabling joint disorder that is mainly characterized by cartilage degeneration and narrow joint spaces. The role of mitochondrial dysfunction in promoting the development of OA has gained much attention. Targeting endogenous molecules to improve mitochondrial function is a potential treatment for OA. Moreover, research on exogenous drugs to improve mitochondrial function in OA based on endogenous molecular targets has been accomplished. In addition, stem cells and exosomes have been deeply researched in the context of cartilage regeneration, and these factors both reverse mitochondrial dysfunctions. Thus, we hypothesize that biomedical approaches will be applied to the treatment of OA. Furthermore, we have summarized the global status of mitochondria and osteoarthritis research in the past two decades, which will contribute to the research field and the development of novel treatment strategies for OA.

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Chondroitin sulfate from sturgeon bone ameliorates pain of osteoarthritis induced by monosodium iodoacetate in rats

Cited by 27 publications

References 28 publications

Fish cartilage: A promising source of biomaterial for biological scaffold fabrication in cartilage tissue engineering

Fish cartilage: A promising source of biomaterial for biological scaffold fabrication in cartilage tissue engineering

Decellularized sturgeon cartilage extracellular matrix scaffold inhibits chondrocyte hypertrophy in vitro and in vivo

Mitochondria: Potential Targets for Osteoarthritis

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