Expressional and functional comparisons of two general odorant binding proteins in Agrotis ipsilon

Huang, Guang-Yao; Liu, Jing Tao; Zhou, Jing; Wang, Qian; Dong, Jian; Zhang, Yong Jun; Li, Xian Chun; Li, Jing; Gu, Siyi

doi:10.1016/j.ibmb.2018.05.003

Cited by 46 publications

(57 citation statements)

References 78 publications

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“…Although OBPs are highly divergent between insects, they contain conserved cysteine residues that are bonded by interlocked disulfide bridges ( Leal et al, 1999 ; Scaloni et al, 1999 ). OBP genes are expressed at higher levels in adult antennae than in other apparatus, suggesting their participation in odorant identification by adult insects ( Chang et al, 2017 ; Huang et al, 2018 ; Zhang et al, 2020 ). The functions of OBPs have been verified by means of in vitro binding experiments in a diverse range of insects such as D. melanogaster ( Larter et al, 2016 ), B. mori ( Zhou et al, 2009 ), Aedes aegypti ( Kim et al, 2017 ), and Periplaneta americana ( He et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Odorant-Binding Proteins Contribute to the Defense of the Red Flour Beetle, Tribolium castaneum, Against Essential Oil of Artemisia vulgaris

et al. 2020

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The function of odorant-binding proteins (OBPs) in insect chemodetection has been extensively studied. However, the role of OBPs in the defense of insects against exogenous toxic substances remains elusive. The red flour beetle, Tribolium castaneum , a major pest of stored grains, causes serious economic losses for the agricultural grain and food processing industries. Here, biochemical analysis showed that essential oil (EO) from Artemisia vulgaris , a traditional Chinese medicine, has a strong contact killing effect against larvae of the red flour beetle. Furthermore, one OBP gene, TcOBPC11 , was significantly induced after exposure to EO. RNA interference (RNAi) against TcOBPC11 led to higher mortality compared with the controls after EO treatment, suggesting that this OBP gene is associated with defense of the beetle against EO and leads to a decrease in sensitivity to the EO. Tissue expression profiling showed that expression of TcOBPC11 was higher in the fat body, Malpighian tubule, and hemolymph than in other larval tissues, and was mainly expressed in epidermis, fat body, and antennae from the early adult. The developmental expression profile revealed that expression of TcOBPC11 was higher in late larval stages and adult stages than in other developmental stages. These data indicate that TcOBPC11 may be involved in sequestration of exogenous toxicants in the larvae of T. castaneum . Our results provide a theoretical basis for the degradation mechanism of exogenous toxicants and identify potential novel targets for controlling the beetle.

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Section: Introductionmentioning

confidence: 99%

Odorant-Binding Proteins Contribute to the Defense of the Red Flour Beetle, Tribolium castaneum, Against Essential Oil of Artemisia vulgaris

et al. 2020

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“…Olfactory sensation is essential for insects to detect air-borne chemical stimuli emitted from host plants, prey, predators, conspecifics and mates. [1][2][3] As the most important olfactory organ, insect antennae have crucial roles in the recognition, desorption, scavenging, protection and transportation of hydrophobic molecules (odorants) across an aqueous environment to olfactory receptor neurons located in sensilla. 4,5 External chemical volatiles including plant odorants and sex pheromones enter the chemosensilla and are captured by odorant-binding proteins (OBPs) in the antennae.…”

Section: Introductionmentioning

confidence: 99%

Odorant‐binding proteins involved in sex pheromone and host‐plant recognition of the sugarcane borer Chilo infuscatellus (Lepidoptera: Crambidae)

Liu

Hu²,

et al. 2020

Pest Management Science

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BACKGROUND: Pheromone-binding proteins (PBPs) are responsible for transporting sex pheromones and general odorantbinding proteins (GOBPs) have been proposed to transport host-plant volatiles. A large number of OBPs have been identified from Lepidoptera species. However, olfactory molecular biology and physiology studies on PBP and GOBP in sugarcane pests are limited. Chilo infuscatellus is one of the most widely distributed pests in sugarcane-producing areas. RESULTS: Three PBPs (CinfPBP1, CinfPBP2 and CinfPBP3) and two GOBPs (CinfGOBP1 and CinfGOBP2) were identified, and five olfactory gene transcripts were abundantly expressed in antennae of C. infuscatellus. Binding assays showed that CinfPBP1-3 exhibited strong binding affinity for the sex pheromone components Z11-16:OH and 16:OH of C. infuscatellus. Meanwhile, Cinf-GOBP1-2 had high binding affinity with host-plant volatiles from sugarcane (Saccharum officinarum). Field-trapping results suggested that four volatile components, octadecane, (Z)-3-hexen-1-ol, ⊍-terpineol and hexadecane from host plants and sex pheromone mixed baits have synergistic roles in attracting C. infuscatellus adult moths. CONCLUSION: Functional characterization of CinfPBPs and CinfGOBPs in C. infuscatellus could help us find new environmentally friendly alternatives to conventional pest control using pesticides in sugarcane fields.

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“…This is in agreement with that reported by Huang et al . (), where in the conventional pheromone communication of Ag. ipsilon (where males seek conspecific females) the expression of GOBP1 and GOBP2 was decreased in males after mating.…”

Section: Discussionmentioning

confidence: 99%

“…These studies represent the first of their class, in which altered OBP expression has been reported to be linked to the controlled exposure of insects to semiochemicals. Furthermore, analysis of the temporal expression of two GOBPs in the black cutworm moth, Agrotis ipsilon , has shown that these proteins reach a maximum level of expression between 3 and 4 days after adult emergence in females (Huang et al ., ). Moreover, their expression was reduced in males after mating, which is in strong agreement with their abolished behaviour to sex attractants after the onset of copulation, as reported in the literature (Gadenne et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Mating‐based regulation and ligand binding of an odorant‐binding protein support the inverse sexual communication of the greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae)

Lizana

Machuca

Larama

et al. 2020

Insect Molecular Biology

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In moths, sex pheromones play a key role in mate finding. These chemicals are transported in the antennae by odorant-binding proteins (OBPs). Commonly, males encounter conspecific females; therefore, several OBPs are male-biased. Less is known, however, about how the olfactory system of moths has evolved toward inverse sexual communication, ie where females seek males. Therefore, the objective of this study was to identify the profile of OBPs and their expression patterns in the bee hive pest, Galleria mellonella, a moth that uses inverse sexual communication. Here, OBP-related transcripts were identified by an RNA Sequencing (RNA-Seq) approach and analysed through both Reverse Transcription Polymerase Chain Reaction (RT-PCR) in different tissues and quantitative real-time PCR for two states, virgin and postmating. Our results indicate that G. mellonella has 20 OBPs distributed amongst different tissues. Interestingly, 17 of the 20 OBPs were significantly down-regulated after mating in females, whereas only OBP7 was up-regulated. By contrast, 18 OBP transcripts were up-regulated in males after mating. Additionally, binding assays and structural simulations showed general odorant-binding protein 2 (GOBP2) was able to bind sex pheromone components and analogues. These findings suggest a possible role of OBPs, especially GOBPs, in the inverse sexual communication of G. mellonella, with gene expression regulated as a response to mating.

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Expressional and functional comparisons of two general odorant binding proteins in Agrotis ipsilon

Cited by 46 publications

References 78 publications

Odorant-Binding Proteins Contribute to the Defense of the Red Flour Beetle, Tribolium castaneum, Against Essential Oil of Artemisia vulgaris

Odorant-Binding Proteins Contribute to the Defense of the Red Flour Beetle, Tribolium castaneum, Against Essential Oil of Artemisia vulgaris

Odorant‐binding proteins involved in sex pheromone and host‐plant recognition of the sugarcane borer Chilo infuscatellus (Lepidoptera: Crambidae)

Mating‐based regulation and ligand binding of an odorant‐binding protein support the inverse sexual communication of the greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae)

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