Multiplex Polymerase Chain Reaction Assay for Screening of Mycotoxin Genes From Ocular Isolates of Fusarium species

Anutarapongpan, Orapin; Maestre-Mesa, Jorge; Alfonso, Eduardo C.; O’Brien, Terrence P.; Miller, Darlene

doi:10.1097/ico.0000000000001607

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…Very little information is currently available regarding the effects DON-producing Fusarium strains have on ocular infections. Anutarapongpan et al [48] conducted a study of 50 different strains of Fusarium spp. involved in fungal keratitis, without finding any DON-or NIV-producing strains.…”

Section: Discussionmentioning

confidence: 99%

Fusarium spp. in Human Disease: Exploring the Boundaries between Commensalism and Pathogenesis

Cighir

Mare

Vultur

et al. 2023

Life

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Fusarium is a large fungal genus that is widely distributed in the environment, mostly known for its plant pathogenicity. Rarely, it is involved in human pathology, where the type of infection caused is highly dependent upon the portal of entry and the immune status of the host. The study at hand aims to summarize routine methods used in diagnosing such infections as well as more advanced molecular diagnostic methods, techniques that can play a huge role in differentiating between colonization and infection when trying to decide the therapeutic outcome. Consequently, to further support our findings, two different strains (one isolated from corneal scrapings and one isolated from purulent discharge) were analyzed in a clinical context and thoroughly tested using classical and modern diagnostic methods: identification by macroscopical and microscopical examinations of the culture and mass spectrometry, completed by molecular methods such as PCR for trichothecene and ERIC-PCR for genetic fingerprinting. Isolation of a clinically relevant Fusarium spp. from a sample still remains a diagnostic challenge for both the clinician and the microbiologist, because differentiating between colonization and infection is very strenuous, but can make a difference in the treatment that is administered to the patient.

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Section: Discussionmentioning

confidence: 99%