Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology

Hakalin, Neumara L.S.; Molina-Gutiérrez, María; Prieto, Alicia; Martı́nez, Marı́a Jesús

doi:10.1016/j.foodchem.2018.04.031

Cited by 20 publications

(11 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analyzed results of RSM model showed that the quadratic model was statistically more sui for the description of the lipase-catalyzed synthesis of 6 -O-vanilloyl-arbutin with the model (p < 0.0001), and the lack of fit (p = 0.4070) was not significant, indicating that the model offers a good fit for the experimental values in the presence of noise. Similarly, Hakalin et al (2018) and Zhu et al (2013) demonstrated the suitability of RSM for enzymatic synthesis of some ester derivatives.…”

Section: Model Fittingmentioning

confidence: 89%

Significantly Enhanced Synthesis of Aromatic Esters of Arbutin Catalyzed by Immobilized Lipase in Co-solvent Systems

Yang

Nie

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Highly efficient and regioselective synthesis of pharmacologically interesting aromatic esters of arbutin catalyzed by immobilized lipase from Penicillium expansum in cosolvent systems was successfully carried out. As compared to tetrahydrofuran solvent, the initial rate and substrate conversion of arbutin vanilylation were markedly enhanced in tetrahydrofuran-isopropyl ether (20%, v/v). Moreover, the effects of three reaction parameters (enzyme amount, temperature and substrate molar ratio of vinyl vanillic acid to arbutin) on 6-O-vanilloyl-arbutin synthesis were scrutinized and the key process parameters were optimized using response surface methodology (RSM). The experimental data were fitted well to a second order polynomial model by using multiple regression analysis. The best combination of variables was 50 • C, 93 U/mL and 11 for the reaction temperature, the enzyme amount and mole ratio of arbutin to vinyl vanilic acid, respectively, and which the reaction rate, substrate conversion and regioselectivity were as high as 8.2 mM/h, 93 and 99%. It was worth noting that a variety of aromatic esters of arbutin were obtained with much higher conversion (93-99%) at these optimal conditions.

show abstract

Section: Model Fittingmentioning

confidence: 89%

Significantly Enhanced Synthesis of Aromatic Esters of Arbutin Catalyzed by Immobilized Lipase in Co-solvent Systems

Yang

Nie

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The response surface method is an optimization method for comprehensive experimental design and mathematical modeling. By testing the representative local points, the regression fits both the functional relationship between the factors in the global scope and the results, and obtains the optimal level of each factor [9]. This study lays a foundation for industrialization of the production process.…”

Section: Introductionmentioning

confidence: 95%

The Synthesis of Mannose-6-Phosphate Using Polyphosphate-Dependent Mannose Kinase

et al. 2019

View full text Add to dashboard Cite

Mannose-6-phosphate (M6P) is involved in many metabolic pathways in life, and it has important applications in the treatment of diseases. This study explored a cost-effective enzyme catalytic synthesis method of M6P, using polyphosphate-dependent mannose kinase from Arthrobacter species. This synthesis uses polyphosphate to replace expensive ATP, and it is greener and safer than chemical synthesis. This study investigated the effects of key factors such as metal ions, temperature, and substrate addition on this enzymatic reaction, and improved the conversion efficiency. We moreover take advantage of the response surface method to explore the best catalytic conditions synthetically. The conversion was 99.17% successful under the optimal reaction conditions. After a series of optimizations, we carried out a 200 mL scale-up experiment, which proved that the method has good prospects for industrial applications.

show abstract

“…In several experiments, Na2CO3 was used as a homogeneous catalyst owing to its prior use in the lignocellulosic biomass HTL to improve bio-oil production (Duan and Savage 2011;Shakya et al 2015). Response surface methodology (RSM) has been widely used to reduce the number of experiments when determining the feasibility of the use of a local material as biomass and to optimize the chemical process (Latchubugata et al 2018;Sulaiman et al 2018;Hakalin et al 2018), especially in bio-oil synthesis (Barbanera et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Bio-oil synthesis from cassava pulp via hydrothermal liquefaction: Effects of catalysts and operating conditions

Nonchana

Pianthong

2020

Int. J. Renew. Energy Dev.

View full text Add to dashboard Cite

The influence of catalysts and operating conditions on the conversion and yield of bio-crude oil from CP via the hydrothermal liquefaction technique (HTL) were studied. HTL is commonly used to convert CP to bio-crude oil (BCO). Three independent factors—reaction temperatures (250–350 °C), reaction times (30–90 min), and CP concentrations (5–20 wt.%)—were investigated. Proximate analysis showed that CP comprises 84.61% volatile matter and 13.59% fixed carbon. The ultimate analysis demonstrated that CP has carbon and oxygen levels of 44.86% and 46.91%, respectively. Thermogravimetric analysis showed that CP begins to decompose at temperatures between 250–350 °C. The results show that KOH is the most suitable catalyst because it provides the highest BCO yield when compared to other catalysts under the same operating conditions. We found that the ideal operating conditions for maximizing BCO performance are 250 °C, pressure of 17.0 MPa, 90 min, 5 wt.%. Under these conditions, Fourier transforms infrared analysis showed that the most abundant chemical bonds found in BCO were CH3-O, CH3-C, and CH3. The findings of the CHNS analysis showed that BCO has an H/C ratio of 2.25, similar to that of petroleum and bio-diesel. Results from a gas chromatograph-mass spectrometer indicate that a fatty acid group is the main component of BCO. ©2020. CBIORE-IJRED. All rights reserved

show abstract

Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology

Cited by 20 publications

References 27 publications

Significantly Enhanced Synthesis of Aromatic Esters of Arbutin Catalyzed by Immobilized Lipase in Co-solvent Systems

Significantly Enhanced Synthesis of Aromatic Esters of Arbutin Catalyzed by Immobilized Lipase in Co-solvent Systems

The Synthesis of Mannose-6-Phosphate Using Polyphosphate-Dependent Mannose Kinase

Bio-oil synthesis from cassava pulp via hydrothermal liquefaction: Effects of catalysts and operating conditions

Contact Info

Product

Resources

About