Target-oriented photofunctional nanoparticles (TOPFNs) for selective photodynamic inactivation of Methicillin-resistant Staphylococcus aureus (MRSA)

Wang, Kang Kyun; Shin, Eon Pil; Lee, Hye Jin; Jung, Seung Jin; Hwang, Jeong Wook; Heo, Il; Kim, Jong Ho; Oh, Min Kyu; Kim, Yong Rok

doi:10.1016/j.jphotobiol.2018.04.037

Cited by 16 publications

(17 citation statements)

References 55 publications

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“…34 The crystallographic structure of MNP was confirmed with an X-ray diffractometer (XRD, Ultima IV, Rigaku) working on Cu Kα radiation. 35 To study the photophysical properties of SHNs, an FT-IR (Vertex 70), a UV−vis spectrophotometer (U-2900, Hitachi), and a spectrofluorometer (F-2500, Hitachi) were utilized. 34 The singlet oxygen generation from SHNs was confirmed with the timeresolved spectroscopic method.…”

Section: Fabrication Of Smart Hybrid Nanoparticle (Shn)mentioning

confidence: 99%

Smart Hybrid Nanocomposite for Photodynamic Inactivation of Cancer Cells with Selectivity

et al. 2019

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Photodynamic therapy has been efficiently applied for cancer therapy. Here, we have fabricated the folic acid (FA)- and pheophorbide A (PA)-conjugated FA/PA@Fe3O4 nanoparticle (smart hybrid nanocomposite, SHN) to enhance the photodynamic inactivation (PDI) of specific cancer cells. SHN coated with the PDI agent is designed to have selectivity for the folate receptor (FR) expressed on cancer cells. Structural characteristics and morphology of the fabricated MNPs were studied with X-ray diffraction and scanning electron microscopy. The photophysical properties of SHN were investigated with absorption, emission spectroscopies, and Fourier transform infrared spectroscopy. In addition, the magnetic property of Fe3O4 nanoparticle (MNP) can be utilized for the collection of SHNs by an external magnetic field. The photofunctionality was given by the photosensitizer, PA, which generates reactive oxygen species by irradiation of visible light. Generation of singlet oxygen was directly evaluated with time-resolved phosphorescence spectroscopy. Biocompatibility and cellular interaction of SHN were also analyzed by using various cancer cells, such as KB, HeLa, and MCF-7 cells which express different levels of FR on the surface. Cellular adsorption and the PDI effect of SHN on the various cancer cells in vitro were correlated well with the surface expression levels of FR, suggesting potential applicability of SHN on specific targeting and PDI of FR-positive cancers.

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Section: Fabrication Of Smart Hybrid Nanoparticle (Shn)mentioning

confidence: 99%

Smart Hybrid Nanocomposite for Photodynamic Inactivation of Cancer Cells with Selectivity

et al. 2019

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“…43 Target-oriented photofunctional nanoparticles were fabricated by an esterification reaction to introduce hematoporphyrin and S. aureus antibody to the surface of Fe 3 O 4 nanoparticles. 44 The results in vitro showed that these MNPs killed selectively S. aureus in L-929 cells and the PDI effect was also confirmed in vivo experiments. Moreover, a nanosystem for early sepsis diagnosis and complete extracorporeal blood disinfection was prepared based on Fe 3 O 4 nanoparticles functionalized with chlorin e6 and bacterial species-identifiable aptamers.…”

Section: Resultsmentioning

confidence: 61%

“…Target-oriented photofunctional nanoparticles were fabricated by an esterification reaction to introduce hematoporphyrin and S. aureus antibody to the surface of Fe 3 O 4 nanoparticles . The results in vitro showed that these MNPs killed selectively S.…”

Section: Results and Discussionmentioning

confidence: 99%

Magnetic Nanoplatforms for in Situ Modification of Macromolecules: Synthesis, Characterization, and Photoinactivating Power of Cationic Nanoiman–Porphyrin Conjugates

Scanone

Gsponer

Alvarez

et al. 2020

ACS Appl. Bio Mater.

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A nanoplatform concept was developed to synthesize accessible photoactive magnetic nanoparticles (MNPs) of Fe 3 O 4 coated with silica. This approach was based on the covalent binding of 5,10,15,20-tetrakis-(pentafluorophenyl)porphyrin (TPPF 20 ) to aminopropyl-grafted MNPs by nucleophilic aromatic substitution reaction (S N Ar) to obtain conjugate MNP− P1. After in situ modification, the remaining pentafluorophenyl groups of TPPF 20 attached to MNPs were substituted by dimethylaminoethoxy groups to form MNP−P2. The basic amine group of these conjugates can be protonated in aqueous media. In addition, MNP−P1 and MNP−P2 were intrinsically charged to produce cationic conjugates MNP + −P1 and MNP + −P2 + by methylation. All of them were easily purified by magnetic decantation in high yields. The average size of the MNPs was ∼15 nm, and the main difference between these conjugates was the greater coating with positive charges of MNP + −P2 + , as shown by the zeta potential values. Absorption spectra exhibited the Soret and Q bands characteristic of TPPF 20 linked to MNPs. Furthermore, these conjugates showed red fluorescence emission of porphyrin with quantum yields of 0.011−0.036. The photodynamic effect sensitized by the conjugates indicated the efficient formation of singlet molecular oxygen in different media, reaching quantum yield values of 0.17−0.34 in N,N-dimethylformamide. The photodynamic activity of the conjugates was evaluated to inactivate the Gram-positive bacteria Staphylococcus aureus, the Gram-negative bacteria Escherichia coli, and the yeast Candida albicans. The modified cationic MNP + −P2 + was the most effective conjugate for photodynamic inactivation (PDI) of microorganisms. Binding of this conjugate to bacteria and photoinactivation capability was checked by means of fluorescence microscopy. Also, sustainable use by recycling was determined after three PDI treatments. Therefore, this methodology is a suitable scaffold for the in situ modification of conjugates, and in particular, MNP + −P2 + represents a useful photodynamic active material to eradicate microorganisms.

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“…16. The delivery units comprise poly-cationic materials, bacterial-targeting peptides [309][310][311][312][313], polymers [314], antibiotics [315], antibodies [316][317][318][319][320] or other proteins [321,322], such that the supramolecular structure acquires higher affinity for specific bacterial components (proteins, sugars,…) thus achieving passive or active targeting [313,319,323,324]. Figure 17 shows an example of targeting achieved by a small cationic antimicrobial peptide (apidaecin Ib) conjugated to the PS 5(40-carboxyphenyl)-10,15,20-triphenylporphyrin.…”

Section: Carriers With Targeting Propertiesmentioning

confidence: 99%

Photodynamic treatment of pathogens

2022

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The current viral pandemic has highlighted the compelling need for effective and versatile treatments, that can be quickly tuned to tackle new threats, and are robust against mutations. Development of such treatments is made even more urgent in view of the decreasing effectiveness of current antibiotics, that makes microbial infections the next emerging global threat. Photodynamic effect is one such method. It relies on physical processes proceeding from excited states of particular organic molecules, called photosensitizers, generated upon absorption of visible or near infrared light. The excited states of these molecules, tailored to undergo efficient intersystem crossing, interact with molecular oxygen and generate short lived reactive oxygen species (ROS), mostly singlet oxygen. These species are highly cytotoxic through non-specific oxidation reactions and constitute the basis of the treatment. In spite of the apparent simplicity of the principle, the method still has to face important challenges. For instance, the short lifetime of ROS means that the photosensitizer must reach the target within a few tens nanometers, which requires proper molecular engineering at the nanoscale level. Photoactive nanostructures thus engineered should ideally comprise a functionality that turns the system into a theranostic means, for instance, through introduction of fluorophores suitable for nanoscopy. We discuss the principles of the method and the current molecular strategies that have been and still are being explored in antimicrobial and antiviral photodynamic treatment.

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Target-oriented photofunctional nanoparticles (TOPFNs) for selective photodynamic inactivation of Methicillin-resistant Staphylococcus aureus (MRSA)

Cited by 16 publications

References 55 publications

Smart Hybrid Nanocomposite for Photodynamic Inactivation of Cancer Cells with Selectivity

Smart Hybrid Nanocomposite for Photodynamic Inactivation of Cancer Cells with Selectivity

Magnetic Nanoplatforms for in Situ Modification of Macromolecules: Synthesis, Characterization, and Photoinactivating Power of Cationic Nanoiman–Porphyrin Conjugates

Photodynamic treatment of pathogens

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