PKC delta activation increases neonatal rat retinal cells survival in vitro: Involvement of neurotrophins and M1 muscarinic receptors

Braga, Luis Eduardo Gomes; Miranda, Renan Lyra; Granja, Marcelo Gomes; Giestal-de-Araujo, Elizabeth; Santos, Aline Araújo dos

doi:10.1016/j.bbrc.2018.04.193

Cited by 9 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PRKCD overexpression reversed the effect of TRIM69 overexpression on BDNF expression. A previous study showed that PRKCD activation upregulated BDNF expression in retinal ganglion cells [37]. These results indicate that TRIM69 negatively regulates BDNF expression in GC cells via PRKCD.…”

Section: Discussionmentioning

confidence: 52%

See 1 more Smart Citation

TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD

Sun,

Chen,

Xia

et al. 2023

Oncogene

View full text Add to dashboard Cite

The tripartite motif (TRIM) protein family has been investigated in multiple human cancers, including gastric cancer (GC). However, the role of TRIM69 in the anoikis resistance and metastasis of GC cells remains to be elucidated. We identi ed the differentially expressed genes in anoikis-resistant GC cells using RNA-sequencing analysis. The interaction between TRIM69 and PRKCD was analyzed by coimmunoprecipitation and mass spectrometry. Our results have shown that TRIM69 was signi cantly downregulated in anoikis-resistant GC cells. TRIM69 overexpression markedly suppressed the anoikis resistance and metastasis of GC cells in vitro and in vivo. TRIM69 knockdown had the opposite effects. Mechanistically, TRIM69 interacted with PRKCD through its B-box domain and catalyzed the K48-linked polyubiquitination of PRKCD. Moreover, TRIM69 inhibited BDNF production in a PRKCD-dependent manner. Importantly, overexpression of PRKCD or BDNF blocked the effects of TRIM69 on the anoikis resistance and metastasis of GC cells. Interestingly, a TRIM69 − PRKCD + BDNF + cell subset was positively associated with metastasis in GC patients. TRIM69-mediated suppression of the anoikis resistance and metastasis of GC cells via modulation of the PRKCD/BDNF axis, with potential implications for novel therapeutic approaches for metastatic GC.

show abstract

Section: Discussionmentioning

confidence: 52%

“…PRKCD is a member of the protein kinase C family of serine-and threonine-speci c protein kinases [36,37] and is involved in the progression of GC [38,39]. For example, PRKCD overexpression enhances the metastasis, chemoresistance, and stem cell-like characteristics of GC cells [38].…”

Section: Discussionmentioning

confidence: 99%

TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD

Sun,

Chen,

Xia

et al. 2023

Oncogene

View full text Add to dashboard Cite

show abstract

“…Therefore, over the last few years, the therapeutic involvement of cholinesterase inhibitors as part of neuroprotective therapy has gained relevance. 81,87,88 Similarly, some experimental studies have demonstrated that the activity of Ach precursors such as citicoline, improves retinal function, restoring the activity of the mitochondrial membrane of RGC, increasing the expression of neural growth factors, and improving cell survival, which confirms that there is a close correlation between retinal function and cholinergic activity [89][90][91] .…”

Section: Cholinergic Activity In the Posterior Segmentmentioning

confidence: 80%

Cholinergic system and the role it plays in the visual system

Durán-Cristiano¹

2022

RMOE

View full text Add to dashboard Cite

The nervous system receives and processes information from the environment thanks to its constant interaction with sensory organs, including vision, a system that includes ocular structures, cortical, and subcortical areas. Consequently, some mediators can receive and transmit visual information so it can be properly processed and interpreted. Among them, the activity of chemical substances called neurotransmitters really stands out, acetylcholine (Ach) in particular since it plays a significant role. Ach acts as a neuromodulator and is involved in the biological events of the visual system that regulate visual functions such as contrast sensitivity, visual perception, and ocular functions like tear production to maintain the homeostasis of the ocular surface. This happens through the interaction of cholinergic receptors on structures of the anterior and posterior segments of the eye. Therefore, the objective of the article is to describe the different aspects that relate to cholinergic activity, the visual system, and its association with the disease.

show abstract

“… 42 , 43 When comparing cPKCγ +/+ +MD mice and cPKCγ −/ − +MD mice, the amplitudes of the a-waves and b-waves in the right eye were not statistically different, indicating that cPKCγ did not affect light transmission of retina. This phenomena might be due to the fact that other subtypes of PKC play an important role in retina function, such as aPKCζ, 44 , 45 nPKCδ, 44 , 46 and cPKCα. 47 , 48 In addition, the intraocular pressures of the MD and control eyes were 8.67 ± 0.380 mm Hg and 8.5 ± 0.365 mm Hg, respectively (n = 6 per group; t = 0.316; degrees of freedom = 10; P = 0.758).…”

Section: Discussionmentioning

confidence: 99%

Monocular Deprivation Affects Visual Cortex Plasticity Through cPKCγ-Modulated GluR1 Phosphorylation in Mice

Zhang

Han

et al. 2020

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

Purpose To determine how visual cortex plasticity changes after monocular deprivation (MD) in mice and whether conventional protein kinase C gamma (cPKCγ) plays a role in visual cortex plasticity. Methods cPKCγ membrane translocation levels were quantified by using immunoblotting to explore the effects of MD on cPKCγ activation. Electrophysiology was used to record field excitatory postsynaptic potential (fEPSP) amplitude with the goal of observing changes in visual cortex plasticity after MD. Immunoblotting was also used to determine the phosphorylation levels of GluR1 at Ser831. Light transmission was analyzed using electroretinography to examine the effects of MD and cPKCγ on mouse retinal function. Results Membrane translocation levels of cPKCγ significantly increased in the contralateral visual cortex of MD mice compared to wild-type (WT) mice ( P < 0.001). In the contralateral visual cortex, long-term potentiation (LTP) and the phosphorylation levels of GluR1 at Ser 831 were increased in cPKCγ +/+ mice after MD. Interestingly, these levels could be downregulated by cPKCγ knockout compared to cPKCγ +/+ +MD mice ( P < 0.001). Compared to the right eyes of WT mice, the amplitudes of a-waves and b-waves declined in deprived right eyes of mice after MD ( P < 0.001). There were no significant differences when comparing cPKCγ +/+ and cPKCγ −/− mice with MD. Conclusions cPKCγ participates in the plasticity of the visual cortex after MD, which is characterized by increased LTP in the contralateral visual cortex, which may be a result of cPKCγ-mediated phosphorylation of GluR1 at Ser 831.

show abstract

PKC delta activation increases neonatal rat retinal cells survival in vitro: Involvement of neurotrophins and M1 muscarinic receptors

Cited by 9 publications

References 41 publications

TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD

TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD

Cholinergic system and the role it plays in the visual system

Monocular Deprivation Affects Visual Cortex Plasticity Through cPKCγ-Modulated GluR1 Phosphorylation in Mice

Contact Info

Product

Resources

About