Gold nanoparticles induce serum amyloid A 1–Toll-like receptor 2 mediated NF-kB signaling in lung cells in vitro

Ng, Cheng Teng; Yip, George W.; Chen, Ee Sin; Poh, Wan Yan Rebecca; Bay, Boon Huat; Yung, Lin‐Yue Lanry

doi:10.1016/j.cbi.2018.04.022

Cited by 10 publications

(8 citation statements)

References 48 publications

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“…As we and others have previously observed, cellular response to NPs usually consists of changes in the expression of genes related to inflammation and apoptosis [34,35,36,37,38]. To further investigate the molecular mechanism of action of NPs on HepG2 cells, in the present research we analysed the DNA methylation status of genes related to inflammation and apoptosis, as well as the expression of miRNAs related to these processes in response to AgNPs, AuNPs and SPIONs at low cytotoxic doses.…”

Section: Introductionmentioning

confidence: 99%

Silver, Gold, and Iron Oxide Nanoparticles Alter miRNA Expression but Do Not Affect DNA Methylation in HepG2 Cells

2019

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The increasing use of nanoparticles (NPs) in various applications entails the need for reliable assessment of their potential toxicity for humans. Originally, studies concerning the toxicity of NPs focused on cytotoxic and genotoxic effects, but more recently, attention has been paid to epigenetic changes induced by nanoparticles. In the present research, we analysed the DNA methylation status of genes related to inflammation and apoptosis as well as the expression of miRNAs related to these processes in response to silver (AgNPs), gold (AuNPs), and superparamagnetic iron oxide nanoparticles (SPIONs) at low cytotoxic doses in HepG2 cells. There were no significant differences between treated and control cells in the DNA methylation status. We identified nine miRNAs, the expression of which was significantly altered by treatment with nanoparticles. The highest number of changes was induced by AgNPs (six miRNAs), followed by AuNPs (four miRNAs) and SPIONs (two miRNAs). Among others, AgNPs suppressed miR-34a expression, which is of particular interest since it may be responsible for the previously observed AgNPs-mediated HepG2 cells sensitisation to tumour necrosis factor (TNF). Most of the miRNAs affected by NP treatment in the present study have been previously shown to inhibit cell proliferation and tumourigenesis. However, based on the observed changes in miRNA expression we cannot draw definite conclusions regarding the pro- or anti-tumour nature of the NPs under study. Further research is needed to fully elucidate the relation between observed changes in miRNA expression and the effect of NPs observed at the cellular level. The results of the present study support the idea of including epigenetic testing during the toxicological assessment of the biological interaction of nanomaterials.

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Section: Introductionmentioning

confidence: 99%

Silver, Gold, and Iron Oxide Nanoparticles Alter miRNA Expression but Do Not Affect DNA Methylation in HepG2 Cells

2019

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“…However, other studies showed opposite results. For example, Ng and coworkers 16 showed that incubation of human bronchial epithelial cells with 50 µg.ml -1 of 20 nm Au NPs activated NF-B in bronchial epithelial cells. Close results were shown in other cell types 17,18 .…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effect of Gold Nanoparticles Supported on Metal Oxides 

Fujita

Zysman

Elgrabli³

et al. 2021

Preprint

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Gold (Au) can be deposited as nanoparticles (NPs) smaller than 10 nm in diameter on a variety of metal oxide (MOx) NPs. Au/MOx NPs have high catalytic performance and selective oxidation capacity which could have implications in terms of biological activity, and more specifically in modulation of the inflammatory reaction. Therefore, the aim of this study was to examine the effect of Au/TiO2, Au/ZrO2 and Au/Ce/O2 on viability, phagocytic capacity and inflammatory profile (TNF-α and IL-1β secretion) of murine macrophages. The most important result of this study is an anti-inflammatory effect of Au/MOx NPs depending on the MOx nature with particle internalization and no alteration of cell viability and phagocytosis. The effect was dependent on the MOx NPs chemical nature (Au/TiO2> Au/ZrO2> Au/CeO2 if we consider the number of cytokines whose concentration was reduced by the NPs), and on the inflammatory mediator considered. The effect of Au/TiO2 NPs was not related to Au NPs size (at least in the case of Au/TiO2 NPs in the range of 3-8 nm). To the best of our knowledge, this is the first demonstration of an anti-inflammatory effect of Au/MOx NPs.

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“…Nanosized gold can be redox-active, with the inherent potential to induce the intracellular formation of reactive oxygen species (ROS) that can correlate with toxicity [19,20]. Reported examples of pulmonary cytotoxicity for gold nanomaterials include an increase in cellular invasion [21], epigenetic modifications [22], organelle reorganisation [23] and changes in protein expression [24,25]. Results in this area are sometimes contradictory and dependent on the characteristics of the materials under investigation.…”

Section: Toxicology and Organ Distributionmentioning

confidence: 99%

Gold nanomaterials in the management of lung cancer

Perry

Wilton‐Ely

Tetley

2020

Emerging Topics in Life Sciences

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Lung cancer (LC) is one of the most deadly cancers worldwide, with very low survival rates, mainly due to poor management, which has barely changed in recent years. Nanomedicines, especially gold nanomaterials, with their unique and size-dependent properties offer a potential solution to many challenges in the field. The versatility afforded by the shape, size, charge and surface chemistry of gold nanostructures allows them to be adapted for many applications in the diagnosis, treatment and imaging of LC. In this review, a survey of the most recent advances in the field is presented with an emphasis on the optical properties of gold nanoscale materials and their use in cancer management. Gold nanoparticle toxicology has also been a focus of interest for many years but the studies have also sometimes arrived at contradictory conclusions. To enable extrapolation and facilitate the development of medicines based on gold nanomaterials, it must be assumed that each design will have its own unique characteristics that require evaluation before translation to the clinic. Advances in the understanding and recognition of the molecular signatures of LC have aided the development of personalised medicines. Tailoring the treatment to each case should, ideally increase the survival outcomes as well as reduce medical costs. This review seeks to present the potential of gold nanomaterials in LC management and to provide a unified view, which will be of interest to those in the field as well as researchers considering entering this highly important area of research.

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Gold nanoparticles induce serum amyloid A 1–Toll-like receptor 2 mediated NF-kB signaling in lung cells in vitro

Cited by 10 publications

References 48 publications

Silver, Gold, and Iron Oxide Nanoparticles Alter miRNA Expression but Do Not Affect DNA Methylation in HepG2 Cells

Silver, Gold, and Iron Oxide Nanoparticles Alter miRNA Expression but Do Not Affect DNA Methylation in HepG2 Cells

Anti-Inflammatory Effect of Gold Nanoparticles Supported on Metal Oxides

Gold nanomaterials in the management of lung cancer

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Gold nanoparticles induce serum amyloid A 1–Toll-like receptor 2 mediated NF-kB signaling in lung cells in vitro

Cited by 10 publications

References 48 publications

Silver, Gold, and Iron Oxide Nanoparticles Alter miRNA Expression but Do Not Affect DNA Methylation in HepG2 Cells

Silver, Gold, and Iron Oxide Nanoparticles Alter miRNA Expression but Do Not Affect DNA Methylation in HepG2 Cells

Anti-Inflammatory Effect of Gold Nanoparticles Supported on Metal Oxides&nbsp;

Gold nanomaterials in the management of lung cancer

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Anti-Inflammatory Effect of Gold Nanoparticles Supported on Metal Oxides