Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes

Chitneedi, P. K.; Suárez-Vega, Aroa; Martínez‐Valladares, María; Arranz, Juan José

doi:10.1186/s13567-018-0534-x

Cited by 26 publications

(32 citation statements)

References 52 publications

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“…Furthermore, the correlations between RNA-Seq and the mRNA expression level from qRT-PCR were relatively high. Until now, RNA-Seq has been successfully applied to transcriptome analysis in the sheep [11–14]. In the current study, by comparative analysis and imperative validation, 622 differentially expressed genes were identified by pairwise comparison.…”

Section: Discussionmentioning

confidence: 99%

“…With the development of high-throughput sequencing technologies, especially RNA-Seq has been widely utilized to explore potential candidate genes which affect important economic traits in animals. In sheep, many studies of RNA-Seq have been conducted using muscle [11], adipose tissues [12], ovarian tissues [13], and abomasal mucosal [14]. However, limited studies on the transcriptome of liver tissues have been reported.…”

Section: Introductionmentioning

confidence: 99%

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Use of short-term high energy dietary for estimating transcriptional profiling of liver tissues in sheep

Yang

Guo

et al. 2019

Preprint

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28This study aimed to evaluate if short-term high energy dietary has any 29 stimulatory effects on liver function and metabolic status in sheep. The experiment 30 was carried out using 30 Dorset×Han crossbred ewes (age, 9 ± 0.6 months; weight, 31 36.58 ± 2.56kg) allocated into two treatments, the control group (DE 11.72 MJ/d; DP 32 79.71 g/d) and the high energy group (DE18.75 MJ/d; DP 108.44 g/d), respectively. 33 Experiment lasted 20 days, including 10 d for adaption. Blood samples of these ewes 34 were collected to detect the concentrations of glucose, insulin, leptin, and cholesterol, 35 respectively. Then, animals were sacrificed and optimal liver samples subjected to 36 explore the genome-wide transcriptome analysis. Results showed that the weight gain 37 was significantly increased in the high energy group, compared with those in the 38 control group (p< 0.01). The concentrations of glucose, insulin, leptin, and cholesterol 39 were also influenced by short-term nutritional supplementation at different levels. 40 Subsequently, 622 differentially expressed genes were identified by pairwise 41 comparison. Of these, 271 genes were down regulated while 351 genes were up 42 regulated. qRT-PCR analysis of 10 randomly selected genes were consistent with the 43 sequencing results. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and 44 Genomes (KEGG) pathways revealed 12 DEGs (including PDK4, ABCA9, 45 ALDH6A1, SLC45A3, G0S2, PPARGC1, GHRHR, GHR, DGKI, SOCS2, LPIN1 and 46 CSKMT) were significantly enriched in cellular carbohydrate catabolic and metabolic 47 process, phosphorelay sensor and phosphotransferase kinase activity, generation of 48 precursor metabolites and energy, lipid metabolic and transport process, positive 3 49 regulation of cellular metabolic process, acyl-CoA desaturase activity and 50 monosaccharide metabolic process. Additionally, we concluded an interaction 51 network related to energy metabolism, which might be contributed to elucidate the 52 precise molecular mechanisms of related genes associated with energy metabolism in 53 the liver tissues of sheep. 54 INTRODUCTION 56Nutrition plays a crucial role in regulating the growth and reproductive 57 performance of farm animals. Energy homeostasis exerts a significant influence on 58 animal health and body condition [1]. Positive energy balance results in increased 59 leptin and insulin concentrations in the blood, which subsequently increased glucose 60 uptake. Previous studies have shown that short-term nutritional supplementation for 4 61 to 11 days exerts significant influences on the blood metabolites and the reproductive 62 performance of ewes [2][3]. Additionally, it is suggested that nutritional 63 supplementation can directly increase the concentrations of several metabolic 64 hormones in ewes, including glucose, insulin, IGF-1, and leptin [4]. However, to our 65 knowledge, there are few studies on the effect of nutritional supplementation on the 66 liver growth, especially its specific regulatory mechanism...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of short-term high energy dietary for estimating transcriptional profiling of liver tissues in sheep

Yang

Guo

et al. 2019

Preprint

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“…One major problem for goats and sheep genomic analysis was that the functional analysis was not available with goats or sheep as reference species due to the lack of data for C. hircus and Ovis aries in functional analysis programs. Therefore, almost all previous publications in goats and sheep genomic filled used human [27][28][29] or bovine [30] genome as reference for functional analysis.…”

Section: Discussionmentioning

confidence: 99%

Genomic variants from RNA-seq for goats resistant or susceptible to gastrointestinal nematode infection

Aboshady¹,

Mandonnet²,

Johansson³

et al. 2019

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Background Gastrointestinal nematodes (GIN) are an important constraint in grazing small ruminant production. Genetic selection for resistant animals is a potential sustainable control strategy. Advances in molecular genetics have led to the identification of several molecular genetic markers associated with genes affecting economic relevant traits.Results In this study, the variants in the genome of Creole goats resistant or susceptible to GIN were discovered from RNA-sequencing. We identified SNPs, insertions and deletions that distinguish the two genotypes, resistant and susceptible and we characterized these variants through functional analysis. The T cell receptor signalling pathway was one of the top significant pathways that distinguish the resistant from the susceptible genotype with 78% of the genes involved in this pathway showing genomic variants.Conclusions These genomic variants are expected to provide useful resources especially for molecular breeding for GIN resistance in goats.

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“…In this study, we have exploited the information derived from an RNA‐Seq experiment with the aim of identifying potential causal mutations related to GIN resistance in sheep. For that, after the general characterisation of the transcriptomic variants of the abomasal lymph node of 12 adult Churra sheep after an experimental infection with Teladorsagia circumcincta (Chitneedi et al ), we performed a detailed analysis of the variants identified in critical genome regions, related to QTL and candidate genes for parasite resistance. Hence, we provide here a list of potential functional variants within immune‐related genes that could be considered, e.g.…”

Section: Introductionmentioning

confidence: 99%

Identification of potential functional variants underlying ovine resistance to gastrointestinal nematode infection by using RNA‐Seq

et al. 2020

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Summary In dairy sheep flocks from Mediterranean countries, replacement and adult ewes are the animals most affected by gastrointestinal nematode (GIN) infections. In this study, we have exploited the information derived from an RNA‐Seq experiment with the aim of identifying potential causal mutations related to GIN resistance in sheep. Considering the RNA‐Seq samples from 12 ewes previously classified as six resistant and six susceptible animals to experimental infection by Teladorsagia circumcincta, we performed a variant calling analysis pipeline using two different types of software, gatk version 3.7 and Samtools version 1.4. The variants commonly identified by the two packages (high‐quality variants) within two types of target regions – (i) QTL regions previously reported in sheep for parasite resistance based on SNP‐chip or sequencing technology studies and (ii) functional candidate genes selected from gene expression studies related to GIN resistance in sheep – were further characterised to identify mutations with a potential functional impact. Among the genes harbouring these potential functional variants (930 and 553 respectively for the two types of regions), we identified 111 immune‐related genes in the QTL regions and 132 immune‐related genes from the initially selected candidate genes. For these immune‐related genes harbouring potential functional variants, the enrichment analyses performed highlighted significant GO terms related to apoptosis, adhesion and inflammatory response, in relation to the QTL related variants, and significant disease‐related terms such as inflammation, adhesion and necrosis, in relation to the initial candidate gene list. Overall, the study provides a valuable list of potential causal mutations that could be considered as candidate causal mutations in relation to GIN resistance in sheep. Future studies should assess the role of these suggested mutations with the aim of identifying genetic markers that could be directly implemented in sheep breeding programmes considering not only production traits, but also functional traits such as resistance to GIN infections.

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Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodes

Cited by 26 publications

References 52 publications

Use of short-term high energy dietary for estimating transcriptional profiling of liver tissues in sheep

Use of short-term high energy dietary for estimating transcriptional profiling of liver tissues in sheep

Genomic variants from RNA-seq for goats resistant or susceptible to gastrointestinal nematode infection

Identification of potential functional variants underlying ovine resistance to gastrointestinal nematode infection by using RNA‐Seq

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