Structural and functional analysis of two novel somatostatin receptors identified from topmouth culter ( Erythroculter ilishaeformis )

Dong, Hui; Wei, Yunhai; Xie, Chang; Zhu, Xiaoxuan; Sun, Chao; Fu, Qianwen; Pan, Lei; Wu, Mengting; Guo, Yinghan; Sun, Jianwei; Shen, Hong; Ye, Jinyun

doi:10.1016/j.cbpc.2018.04.004

Cited by 5 publications

(2 citation statements)

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“…Topmouth culter ( Erythroculter ilishaeformis ) is a kind of freshwater fish belonging to the genera of Culter fishes ( Dong et al, 2017 ). It is one of the important economical fishes in Taihu Lake, which is the third largest freshwater lake in China ( Dong et al, 2018 ). Topmouth culter, whitebait ( Neosalanx taihuensis ), and white shrimp ( Fenneropenaeus chinensis ) are well-known as “three whites” of Taihu Lake that provide a lot of dietary protein for people ( Dong et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Anti-infective Effects of a Fish-Derived Antimicrobial Peptide Against Drug-Resistant Bacteria and Its Synergistic Effects With Antibiotic

Chen

Cheng

et al. 2020

Front. Microbiol.

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Antimicrobial peptides (AMPs) play pivotal roles in protecting against microbial infection in fish. However, AMPs from topmouth culter (Erythroculter ilishaeformis) are rarely known. In our study, we isolated an AMP from the head kidney of topmouth culter, which belonged to liver-expressed antimicrobial peptide 2 (LEAP-2) family. Topmouth culter LEAP-2 showed inhibitory effects on aquatic bacterial growth, including antibiotic-resistant bacteria, with minimal inhibitory concentration values ranging from 18.75 to 150 μg/ml. It was lethal for Aeromonas hydrophila (resistant to ampicillin), and took less than 60 min to kill A. hydrophila at a concentration of 5 × MIC. Scanning electron microscope (SEM) and SYTOX Green uptake assay indicated that it impaired the integrity of bacterial membrane by eliciting pore formation, thereby increasing the permeabilization of bacterial membrane. In addition, it showed none inducible drug resistance to aquatic bacteria. Interestingly, it efficiently delayed ampicillin-induced drug resistance in Vibrio parahaemolyticus (sensitive to ampicillin) and sensitized ampicillin-resistant bacteria to ampicillin. The chequerboard assay indicated that topmouth culter LEAP-2 generated synergistic effects with ampicillin, indicating the combinational usage potential of topmouth culter LEAP-2 with antibiotics. As expected, topmouth culter LEAP-2 significantly alleviated ampicillin-resistant A. hydrophila infection in vivo, and enhanced the therapeutic efficacy of ampicillin against A. hydrophila in vivo. Our findings provide a fish innate immune system-derived peptide candidate for the substitute of antibiotics and highlight its potential for application in antibiotic-resistant bacterial infection in aquaculture industry.

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Section: Introductionmentioning

confidence: 99%

Anti-infective Effects of a Fish-Derived Antimicrobial Peptide Against Drug-Resistant Bacteria and Its Synergistic Effects With Antibiotic

Chen

Cheng

et al. 2020

Front. Microbiol.

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“…The systemic function of this peptide is inhibition of the release of growth hormone, insulin, glucagon, gastrin, secretin and cholecystokinin [11][12][13]. Five somatostatin receptors (SSTR1-5) [14] have been characterized in mammals, and six receptors in fishes (SSTR1-3, SSTR5-7) [8,15,16]. Binding of somatostatin to each of the receptor subtypes results in inhibition of adenylate cyclase (AC) in vertebrates [17].…”

Section: Introductionmentioning

confidence: 99%

Receptory somatostatynowe w mózgu

et al. 2018

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Somatostatyna jest peptydem uczestniczącym w wielu szlakach biochemicznych i sygnałowych. Działa ona za pośrednictwem pięciu receptorów (SSTR 1-5) należących do rodziny receptorów sprzężonych z białkiem G. Wszystkie receptory somatostatynowe charakteryzują się znacznym stopniem homologii w budowie molekularnej i mogą oddziaływać ze sobą oraz niektórymi innymi receptorami tworząc strukturalne i funkcjonalne homo- lub heterodimery. Efekty komórkowe wywierane przez agonistów SSTR w tkankach obwodowych polegają głównie na hamowaniu uwalniania hormonów. W układzie nerwowym somatostatyna pełni również funkcje neuromodulatora i neurotransmitera wpływając na procesy pamięci, uczenia się oraz nastrój. SSTR są zaangażowane w regulację procesów fizjologicznych takich jak odczuwanie świądu i bólu, funkcje rozrodcze i pobieranie pokarmu. Poza funkcjami fizjologicznymi SSTR mają swój udział również w patogenezie i przebiegu nowotworów pochodzenia glejowego, chorób neurodegeneracyjnych czy zmian po udarze krwotocznym. Ostatnie lata dostarczają coraz więcej badań dotyczących roli szlaków sygnalizacyjnych receptorów somatostatynowych w mózgu, a wiedza na ten temat wciąż dynamicznie się rozwija.

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