Quantitative Analysis of Four Catechins from Green Tea Extract in Human Plasma Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry for Pharmacokinetic Studies

Abstract

Green tea is consumed as a beverage worldwide and has beneficial effects, such as a lower risk of cardiovascular disease and cancer. A quantitative analysis of the beneficial components in plasma is important for understanding the potential health benefits of green tea. Four catechins—epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG), epigallocatechin (EGC), and epicatechin (EC)—which account for the majority of the components of green tea, were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). In this study, a validated method was optimized to obtain the blood concentrations after the one-time ingestion of 630 mg green tea extract with digoxin and then after the ingestion of 630 mg green tea repeatedly for 15 days. The calibration curve, including the LLOQ, was constructed over 1–500 ng/mL for EGCG, ECG, and EGC and 0.1–50 ng/mL for EC. The method for inter- and intra-validation was applied, acceptable for both accuracy and precision. We successfully developed an appropriate UPLC-MS/MS method for human plasma with good reproducibility and sensitivity. Thus, this method could be applied for future preclinical and clinical studies on EGCG, ECG, EGC, and EC.

Method development and validation for rapid identification of epigallocatechin gallate using ultra-high performance liquid chromatography

“…The results obtained from the preliminary optimization are given in Table 1 and it was observed that trial-5 was found to result in good peak shape, met the system suitability requirements, and thus was accepted and evaluated. The chromatogram of EGCG obtained using the studied condition is given in Fig 2A. In the present study, the RT of EGCG peak was 2.1 minutes, which is found to be rapid as compared to previous studies in which RT was reported as 9 minutes and 5 minutes [10,14].…”

“…Previously, methods have been developed to estimate the EGCG using Spectrophotometry [7], High Performance Liquid Chromatography (HPLC) [8,9], Reverse Phase (RP) HPLC [10,11] and Ultra-Performance Liquid Chromatography (UPLC) [12,13], Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) [14], Ultra-Performance Liquid Chromatography/Electrospray Ionization-Mass Spectrometry (UPLC/ESI-MS), Ultra-Performance Liquid Chromatography-Diode Array Detector-Mass Spectroscopy (UPLC-DAD-MS) [15] and Ultra-Performance Liquid Chromatography-Time-of-Flight-Mass Spectrometry (UPLC-TOF-MS) [16]. Also keeping in view of the low bioavailability of the compound and the limited studies to identify EGCG alone from the samples, there is a strong need to develop and validate a simple and rapid method to identify EGCG.…”

Light and Autofluorescence, Multitasking Features in Living Organisms

“…Such compounds are particularly abundant in green and black tea and may have beneficial effects on human health or influence on drug pharmacokinetics in human plasma [114,115]. For these reasons spectral fluorescence analysis has been recently successfully proposed as a valuable support to assess quality and origin of tea [116,117].…”

Quality Evaluation of Green and Dark Tea Grade Using Electronic Nose and Multivariate Statistical Analysis

“…Up till now, many analytical techniques have been developed for the analysis of tea quality. For example, capillary zone electrophoresis (Horie & Kohata, ; Wang, Su, Liang, & Sun, ), ultraperformance liquid chromatography (Moradi, Kiarostami, & Amini, ; Park, Kim, & Shin, ), gas chromatography‐mass spectrometry (Ma et al., ; Yang et al., ), electronic nose (e‐nose), and so on (Banerjee, Chattopadhyay, Tudu, Bhattacharyya, & Bandyopadhyay, ; Wang et al., ; Zhu, Chen, Wang, Niu, & Xiao, ) are widely used in tea quality assessment. Owing to the rapid development of instrumental techniques, component analysis for aroma differences among various teas are becoming less time consuming and laborious (Choudhury, Yabansu, Kalidindi, & Dennstedt, ; Cliff, Law, Luecker, Scaman, & Kermode, ; Marguí & Voutchkov, ).…”