Differential effects of lipopolysaccharide on mouse sensory TRP channels

Boonen, Brett; Alpízar, Yeranddy A.; Sánchez, Ana; López‐Requena, Alejandro; Voets, Thomas; Talavera, Karel

doi:10.1016/j.ceca.2018.04.004

Cited by 70 publications

(55 citation statements)

References 49 publications

(64 reference statements)

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“…an endotoxin, is the major component of the outer membrane of gram-negative bacteria, and it plays an important role in the pathogenesis of certain bacterial infections as an immunostimulator and immunomodulator. 38,39 Furthermore, lipopolysaccharide can be detoxified by removing the two phosphate groups on lipopolysaccharide carbohydrates, 40 while phosphotransferase system was enriched in the NT group. Thus the enrichment of lipopolysaccharide biosynthesis pathway was harmful to the neonatal health.…”

Section: Discussionmentioning

confidence: 99%

Impact of maternal intrapartum antibiotics on the initial oral microbiome of neonates

Xiao

Zhang

et al. 2019

Pediatrics & Neonatology

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Neonatal oral microbiome; Maternal intrapartum antibiotics; 16S rRNA sequencing Objectives: Prior studies have proposed that maternal intrapartum antibiotic exposure shapes the gut microbiota and, subsequently the child's health. However, the effect of maternal intrapartum antibiotic exposure and its influence on the development of the neonatal oral microbiota in early infancy has not yet been reported. The aim of this study was to compare the initial oral microbiota immediately after birth of healthy infants with and without intrapartum antibiotic exposure. Methods: Twenty-two newborns of the BaoAn Maternal and Child Care Hospital (Shenzhen, China) were recruited for this study, 11 born to mothers without intrapartum antibiotic exposure (NT group) and 11 to mothers with intrapartum antibiotic prophylaxis with cefamezin (AT group). Oral microbiome profiles were determined by 16S rRNA sequencing based on the V3eV4 hyper-variable regions. Results: Phylum Firmicutes was most frequently detected in subjects both groups and a higher frequency was observed in the NT group than the AT group. Phyla Actinobacteria, Bacteroidetes and Proteobacteria were more abundant after intrapartum antibiotics exposure. Genus Lactobacillus belonging to Firmicutes was predominant in the neonates not exposed to antibiotics, while significantly higher percentages of genera Klebsiella, Roseburia, Propionibacterium, Faecalibacterium, Escherichia/Shigella, Corynebacterium, Bifidobacterium, and Bacteroides were noted in AT infants than NT infants. Further function analysis demonstrated that lipopolysaccharide biosynthesis and amino aciderelated metabolic function was enriched in the AT group, and carbohydrate metabolism pathways were more abundant in the NT group. Conclusions: These findings revealed distinctions in both taxa and metabolic function of oral microbiota between antibiotics-treated and unexposed groups, which indicated that maternal intrapartum antibiotic treatment is a key regulator of the initial neonatal oral microbiome.

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Section: Discussionmentioning

confidence: 99%

Impact of maternal intrapartum antibiotics on the initial oral microbiome of neonates

Xiao

Zhang

et al. 2019

Pediatrics & Neonatology

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“…In addition to TRPA1, Boonen et al demonstrated that LPS activates TRPV1 to promote an intracellular calcium influx in HEK293T cells transfected with recombinant human TRPV1 [85]. In the mouse dorsal root ganglion (DRG) neuron, the LPS response that was only partially blunted in Trpa1-/neurons was completely abrogated in Trpa1-/-Trpv1-/-DKO neurons.…”

Section: Lps Recognition By Trp Channelsmentioning

confidence: 99%

“…In the mouse dorsal root ganglion (DRG) neuron, the LPS response that was only partially blunted in Trpa1-/neurons was completely abrogated in Trpa1-/-Trpv1-/-DKO neurons. This suggests involvement of TRPV1 in sensing LPS and neurogenic inflammation [85]. Moreover, using recombinant TRP expression in HEK293T cells, Alenmyr and colleagues demonstrated that, in addition to TRPA1 and TRPV1, LPS weakly activated TRPM3 and TRPM8 to promote calcium influx [86].…”

Section: Lps Recognition By Trp Channelsmentioning

confidence: 99%

Recent Advances in Lipopolysaccharide Recognition Systems

Mazgaeen

Gurung

2020

IJMS

223

156

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Lipopolysaccharide (LPS), commonly known as endotoxin, is ubiquitous and the most-studied pathogen-associated molecular pattern. A component of Gram-negative bacteria, extracellular LPS is sensed by our immune system via the toll-like receptor (TLR)-4. Given that TLR4 is membrane bound, it recognizes LPS in the extracellular milieu or within endosomes. Whether additional sensors, if any, play a role in LPS recognition within the cytoplasm remained unknown until recently. The last decade has seen an unprecedented unfolding of TLR4-independent LPS sensing pathways. First, transient receptor potential (TRP) channels have been identified as non-TLR membrane-bound sensors of LPS and, second, caspase-4/5 (and caspase-11 in mice) have been established as the cytoplasmic sensors for LPS. Here in this review, we detail the brief history of LPS discovery, followed by the discovery of TLR4, TRP as the membrane-bound sensor, and our current understanding of caspase-4/5/11 as cytoplasmic sensors.

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“…The concentration selection of LPS used for the exposure of cells was conducted based on data from literature 11, 12 13 …”

Section: Discussionmentioning

confidence: 99%

Effect of adalimumab on the expression profile of mRNA , and protein associated with JAK / STAT signaling pathway in fibroblast exposed to lipopolysaccharide

Adwent

Schweizer

Grabarek

et al. 2020

Dermatologic Therapy

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The aim of this study was to assess the effect of adalimumab on the expression level of mRNA and protein TNF‐α, IFN‐γ, IL‐17, IL12A, IL12B, and IL23A in the culture of normal human fibroblasts, in which the LPS inflammation process was induced. The NHDF culture was exposed to the effect of LPS in the concentrations of 1, 2, and 10 μg/mL for 2, 8, and 24 hour periods, and then adalimumab was added at the concentration of 8 μg/mL, it was then incubated for 2, 8, and 24 hour. Cells unexposed to LPS and adalimumab constituted the control. The microarray expression techniques, RTqPCR, and ELISA assay were used. Irrespectively of the concentration of LPS used and the incubation time of it with cells overexpression of the analyzed genes is present, with increasing factor concentration used to induce inflammation and incubation time with it, expression of the assessed genes was greater. In turn, adding the anti‐TNF drug to the culture caused the silencing of the expression of the mRNAs and proteins. It was confirmed that LPS and adalimumab above all affect the expression of genes and proteins dependent on the interaction of IL‐12 with receptors, which are TNF‐α and IFN‐γ, and to a lesser extent also modulate IL‐17.

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Differential effects of lipopolysaccharide on mouse sensory TRP channels

Cited by 70 publications

References 49 publications

Impact of maternal intrapartum antibiotics on the initial oral microbiome of neonates

Impact of maternal intrapartum antibiotics on the initial oral microbiome of neonates

Recent Advances in Lipopolysaccharide Recognition Systems

Effect of adalimumab on the expression profile of mRNA , and protein associated with JAK / STAT signaling pathway in fibroblast exposed to lipopolysaccharide

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