Escherichia coli as a host for metabolic engineering

Pontrelli, Sammy; Chiu, Tsan Yu; Lan, Ethan I.; Chen, Frederic Y.-H.; Chang, Peiching; Liao, James C.

doi:10.1016/j.ymben.2018.04.008

Cited by 278 publications

(161 citation statements)

References 434 publications

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“…This is also the case of microorganisms with a long tradition in the fields of microbiology and biotechnology that have managed to reach an industrial scale of production. The (somewhat short) list of bacterial hosts falling into this category includes Escherichia coli (Pontrelli et al ., ), Bacillus subtilis (Gu et al ., ), Streptomyces sp. (Spasic et al ., ), Pseudomonas putida (Nikel and de Lorenzo, ), and Corynebacterium glutamicum (Wendisch et al ., ), for which extensive background fundamental knowledge has been amassed.…”

Section: Desirable Properties In the Ideal Bacterial Chassis: Bridginmentioning

confidence: 99%

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Calero

Nikel

2018

Microbial Biotechnology

227

184

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The last few years have witnessed an unprecedented increase in the number of novel bacterial species that hold potential to be used for metabolic engineering. Historically, however, only a handful of bacteria have attained the acceptance and widespread use that are needed to fulfil the needs of industrial bioproduction - and only for the synthesis of very few, structurally simple compounds. One of the reasons for this unfortunate circumstance has been the dearth of tools for targeted genome engineering of bacterial chassis, and, nowadays, synthetic biology is significantly helping to bridge such knowledge gap. Against this background, in this review, we discuss the state of the art in the rational design and construction of robust bacterial chassis for metabolic engineering, presenting key examples of bacterial species that have secured a place in industrial bioproduction. The emergence of novel bacterial chassis is also considered at the light of the unique properties of their physiology and metabolism, and the practical applications in which they are expected to outperform other microbial platforms. Emerging opportunities, essential strategies to enable successful development of industrial phenotypes, and major challenges in the field of bacterial chassis development are also discussed, outlining the solutions that contemporary synthetic biology-guided metabolic engineering offers to tackle these issues.

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Section: Desirable Properties In the Ideal Bacterial Chassis: Bridginmentioning

confidence: 99%

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Calero

Nikel

2018

Microbial Biotechnology

227

184

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“…Many industrial microorganisms have been engineered as excellent production strains for CIB, such as Bacillus subtilis , E. coli , Corynebacterium glutamicum , Pseudomonas putida , Saccharomyces cerevisiae ( S. cerevisiae ) and so forth . Food supplements and fine chemicals such as vitamins, steroids, amino acids; biofuels including ethanol, butanol, biodiesel, and hydrogen; materials such as polyhydroxyalkanoates (PHA) and polysaccharides; polymer precursors like lactic acid (LA) succinic acid, and so forth.…”

Section: Current Industrial Biotechnologymentioning

confidence: 99%

Next‐Generation Industrial Biotechnology‐Transforming the Current Industrial Biotechnology into Competitive Processes

Chen

2019

Biotechnology Journal

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The chemical industry has made a contribution to modern society by providing cost‐competitive products for our daily use. However, it now faces a serious challenge regarding environmental pollutions and greenhouse gas emission. With the rapid development of molecular biology, biochemistry, and synthetic biology, industrial biotechnology has evolved to become more efficient for production of chemicals and materials. However, in contrast to chemical industries, current industrial biotechnology (CIB) is still not competitive for production of chemicals, materials, and biofuels due to their low efficiency and complicated sterilization processes as well as high‐energy consumption. It must be further developed into “next‐generation industrial biotechnology” (NGIB), which is low‐cost mixed substrates based on less freshwater consumption, energy‐saving, and long‐lasting open continuous intelligent processing, overcoming the shortcomings of CIB and transforming the CIB into competitive processes. Contamination‐resistant microorganism as chassis is the key to a successful NGIB, which requires resistance to microbial or phage contaminations, and available tools and methods for metabolic or synthetic biology engineering. This review proposes a list of contamination‐resistant bacteria and takes Halomonas spp. as an example for the production of a variety of products, including polyhydroxyalkanoates under open‐ and continuous‐processing conditions proposed for NGIB.

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“…As a mainstream host for metabolic engineering, [9] Escherichia coli possesses several advantages with regards to polyketide production. Of highest interest, this host is the most genetically tractable and thus allows for the rapid testing of constructs and use of bacterially derived biosensors for strain optimization.…”

Section: Escherichia Colimentioning

confidence: 99%

Expanding the Chemical Palette of Industrial Microbes: Metabolic Engineering for Type III PKS‐Derived Polyketides

Palmer

Alper

2018

Biotechnology Journal

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Polyketides are a unique class of molecules with attractive bioactive and chemical properties. As a result, biorenewable production is being explored with these molecules as potential pharmaceutical, fuel, and material precursors. In particular, type III polyketide synthases enable access to a diverse class of chemicals using a relatively simple biochemical synthesis pathway. In this review, the recent advances in the engineering of microbial hosts for the production of type III PKS‐derived polyketides are highlighted. In particular, the field has moved beyond simple proof‐of‐concept and has been exploring engineering efforts that have led to improved production scales. This review details engineering progress for the production of acetyl‐CoA‐ and malonyl‐CoA‐derived polyketides including the products triacetic acid lactone and phloroglucinol as well as polyphenolic, phenylpropanoid‐derived compounds including flavonoids, stilbenoids, and curcuminoids. Specifically, the authors focus on enumerating the metabolic engineering strategies employed and product titers achieved for these molecules. Finally, the authors highlight tools and strategies that can be leveraged to realize the potential of microbial production and diversification of these molecules.

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Escherichia coli as a host for metabolic engineering

Cited by 278 publications

References 434 publications

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Next‐Generation Industrial Biotechnology‐Transforming the Current Industrial Biotechnology into Competitive Processes

Expanding the Chemical Palette of Industrial Microbes: Metabolic Engineering for Type III PKS‐Derived Polyketides

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