Age‐Dependent Protective Effect of Selenium against UVA Irradiation in Primary Human Keratinocytes and the Associated DNA Repair Signature

Abstract

Few studies have focused on the protective role of selenium (Se) against skin aging and photoaging even though selenoproteins are essential for keratinocyte function and skin development. To the best of our knowledge, the impact of Se supplementation on skin cells from elderly and young donors has not been reported. Therefore, the main objective of our study was to evaluate the effects of Se supplementation on skin keratinocytes at baseline and after exposure to ultraviolet A (UVA) irradiation. Low doses of Se (30 nM) were very potently protective against UVA-induced cytotoxicity in young keratinocytes, whereas the protection efficiency of Se in old keratinocytes required higher concentrations (240 nM). Additionally, the DNA repair ability of the old keratinocytes drastically decreased compared with that of the young keratinocytes at baseline and after the UVA exposure. The Se supplementation significantly enhanced the DNA repair of 8-oxoguanine (8oxoG) only in the keratinocytes isolated from young donors. Therefore, aged keratinocytes have an increased vulnerability to oxidative DNA damage, and the Se needs in the elderly should be considered. Strengthening DNA repair activities with Se supplementation may represent a new strategy to combat aging and skin photoaging.

Prospective Evaluation of the Efficacy of a Food Supplement in Increasing Photoprotection and Improving Selective Markers Related to Skin Photo-Ageing

“…Consequently, the treatment was not interrupted by any of the study participants. The methods used to evaluate product tolerability have been previously validated in relevant studies [51][52][53]. Apart from the two adverse events previously mentioned, all the investigated aspects concerning the product were positively assessed by enrolled subjects.…”

Efficacy and Safety of an Oral Nutritional (Dietary) Supplement Containing Pinus pinaster Bark Extract and Grape Seed Extract in Combination with a High SPF Sunscreen in the Treatment of Mild-to-Moderate Melasma: A Prospective Clinical Study

“…A novel oral nutritional (dietary) supplement based on Pinus pinaster bark extract and proanthocyanidin-rich grape seed extract, has been developed to be incorporated in the armamentarium for the treatment of melasma. Vitamin E and C, selenium and nicotinamide have also been shown to reduce the negative effects of UV radiation on skin by eliminating reactive oxygen species and reducing the erythema associated with UV [18,20,21].…”

Bioactive Compounds for Skin Health: A Review

“…This was confirmed in a study by Jobeili et al, which showed that selenium delays skin ageing by protecting keratinocyte stem cells [ 91 ]. Favrot et al showed that low doses of Se (30 nM) provide potent protection against UVA-induced cytotoxicity in young keratinocytes (from 20–30-year-old donors), while higher concentrations (240 nM) were required for protective efficacy in old keratinocytes (from donors 60–70 years old) [ 92 ].…”

“…Selenium supplementation is an important strategy for inhibiting wrinkles, because it can reverse ultraviolet light damage and play an anti-ageing role in the skin [ 89 , 92 ]. Kim et al showed that a selenium-rich tuna heart extract enhanced collagen synthesis and promoted the proliferation of skin fibroblasts, thus having anti-ageing and anti-wrinkle effects [ 93 ].…”