Phylodynamics of Merkel-cell polyomavirus and human polyomavirus 6: A long-term history with humans

Torres, Carolina; Barrios, Melina Elizabeth; Cammarata, Robertina Viviana; Victoria, Matías; Fernández-Cassi, Xavier; Bofill-Mas, Sı́lvia; Colina, Rodney; Fernández, María Dolores Blanco; Mbayed, Viviana Andrea

doi:10.1016/j.ympev.2018.04.025

Cited by 13 publications

(10 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our population, a slightly different general prevalence of HPyV but a very similar prevalence of MCPyV was observed, confirming that the virus is widespread in the adult population [32]. Sequence analysis confirmed the high homology of the strains amplified in our population with strains previously isolated from the healthy skin of the worldwide population [33,34].…”

Section: Discussionsupporting

confidence: 82%

Merkel Cell Polyomavirus Is Associated with Anal Infections in Men Who Have Sex with Men

et al. 2019

View full text Add to dashboard Cite

Background: Viral infections of the anal/rectal tract of men who have sex with men (MSM) have been poorly studied. Methods: In total, 158 swab samples (81 anal/rectal, 65 throat/oral and 12 urethral) were collected from 126 MSM. DNA was isolated and subjected to real-time PCR assays for the detection of the sexually transmitted (ST) pathogens Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasmas ssp, human papillomavirus (HPV) and six human polyomaviruses (HPyVs; JCPyV, BKPyV, Merkel cell PyV–MCPyV-, HPyV-6, HPyV-7 and HPyV-9). Results: C. trachomatis (31/126, 24.6%) and M. genitalium (30/126, 23.8%) were the most frequently detected ST pathogens. Thirty-one/126 (24.6%) patients were positive for at least one HPyV. The significantly (p < 0.05) prevalent HPyV in the anal tract was MCPyV, which was amplified in 27/81 (33.3%) samples, followed by HPyV-6, which was amplified in 6/81 (7.4%) swabs. Coinfections with MCPyV and C. trachomatis or Mycoplasmas were found in 4/21 (19.0%) and 5/21 (23.8%) anal/rectal swabs, respectively. Three/4 MCPyV-C. trachomatis coinfected patients were symptomatic. Conclusions: Based on the high prevalence of MCPyV in the anal/rectal swabs from MSM patients and on the well-known oncogenic properties of MCPyV, sexual transmission and possible involvement of HPyVs in the pathogenesis of diseases of the anal canal should be further studied.

show abstract

Section: Discussionsupporting

confidence: 82%

Merkel Cell Polyomavirus Is Associated with Anal Infections in Men Who Have Sex with Men

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Likewise, the F peptide a.a. chain was 100% identical to that of VPs from North American and European isolates ( 4 , 66 ). Moreover, the a.a. sequence of F peptide was 96% (24/25 a.a.) concordant with that of VPs from the Japanese and Chinese isolates (F to S at the last a.a. position of the peptide, n.25) ( 4 , 66 ), as well as with that from AHW79949 and AWG42110 VP strains (T to I at a.a. position n.5 of the peptide in both cases) ( 67 , 68 ).The identity with the VP native strains from other 15 PyVs was below 37.5% and 36% for S and F peptide, respectively. In addition, a total of 12 and 8 different PyV types shared less than 16.6% and 16% of identity with the a.a. strains from S and F peptides, respectively ( Figures 1A, C ).…”

Section: Resultsmentioning

confidence: 95%

Serum Antibodies Against the Oncogenic Merkel Cell Polyomavirus Detected by an Innovative Immunological Assay With Mimotopes in Healthy Subjects

Mazziotta¹,

Lanzillotti²,

Torreggiani³

et al. 2021

Front. Immunol.

View full text Add to dashboard Cite

Merkel cell polyomavirus (MCPyV), a small DNA tumor virus, has been detected in Merkel cell carcinoma (MCC) and in normal tissues. Since MCPyV infection occurs in both MCC-affected patients and healthy subjects (HS), innovative immunoassays for detecting antibodies (abs) against MCPyV are required. Herein, sera from HS were analyzed with a novel indirect ELISA using two synthetic peptides mimicking MCPyV capsid protein epitopes of VP1 and VP2. Synthetic peptides were designed to recognize IgGs against MCPyV VP mimotopes using a computer-assisted approach. The assay was set up evaluating its performance in detecting IgGs anti-MCPyV on MCPyV-positive (n=65) and -negative (n=67) control sera. Then, the ELISA was extended to sera (n=548) from HS aged 18-65 yrs old. Age-specific MCPyV-seroprevalence was investigated. Performance evaluation indicated that the assay showed 80% sensitivity, 91% specificity and 83.9% accuracy, with positive and negative predictive values of 94.3% and 71%, respectively. The ratio expected/obtained data agreement was 86%, with a Cohen’s kappa of 0.72. Receiver-operating characteristic (ROC) curves analysis indicated that the areas under the curves (AUCs) for the two peptides were 0.82 and 0.74, respectively. Intra-/inter-run variations were below 9%. The overall prevalence of serum IgGs anti-MCPyV in HS was 62.9% (345/548). Age-specific MCPyV-seroprevalence was 63.1% (82/130), 56.7% (68/120), 64.5% (91/141), and 66.2% (104/157) in HS aged 18-30, 31-40, 41-50 and 51-65 yrs old, respectively (p>0.05). Performance evaluation suggests that our indirect ELISA is reliable in detecting IgGs anti-MCPyV. Our immunological data indicate that MCPyV infection occurs asymptomatically, at a relatively high prevalence, in humans.

show abstract

“…However, elevated substitution rates have been described for small dsDNA viruses with the increasing number of sequence data, the development of analysis tools and with the examination of viral evolution independently from the host (Domingo‐Calap et al., 2018; Firth et al., 2010). Recent studies determined evolutionary rates in the order of 10 –8 –10 –3 for dsDNA viruses, including polyomaviruses, emphasizing that these are calculated values for a given data set (Aiewsakun & Katzourakis, 2016; Torres et al., 2018). In our study, a complete genome‐based evolutionary rate in the order of 10 –5 s/s/y was estimated for the GHPV with purifying selection acting on its genes.…”

Section: Resultsmentioning

confidence: 99%

“…To evaluate the evolutionary mechanisms of GHPV on a spatiotemporal scale, we used sampling dates and geographic origin of Polyomaviruses are described as slowly evolving viruses with conserved genomic sequences that helps co-divergence with limited number of host species (Torres et al, 2018). However, elevated substitution rates have been described for small dsDNA viruses with the increasing number of sequence data, the development of analysis tools and with the examination of viral evolution independently from the host (Domingo-Calap et al, 2018;Firth et al, 2010).…”

Section: Re Sults and Discussionmentioning

confidence: 99%

Molecular epidemiology and phylodynamics of goose haemorrhagic polyomavirus

Kaszab

Marton

Dán

et al. 2020

Transbound. Emerg. Dis.

View full text Add to dashboard Cite

The causative dsDNA virus, the goose haemorrhagic polyomavirus (GHPV), or recently named Anser anser polyomavirus 1, belongs to the Gammapolyomavirus genus of the Polyomaviridae family (Guerin et al., 2000). The genome of GHPV is 5252-5256 bp long and comprises the genes of the VP1-VP3 structure proteins, the large and small tumour antigens (LTA and STA), and the predicted ORF-X with unknown function (Fehér,

show abstract

Phylodynamics of Merkel-cell polyomavirus and human polyomavirus 6: A long-term history with humans

Cited by 13 publications

References 60 publications

Merkel Cell Polyomavirus Is Associated with Anal Infections in Men Who Have Sex with Men

Merkel Cell Polyomavirus Is Associated with Anal Infections in Men Who Have Sex with Men

Serum Antibodies Against the Oncogenic Merkel Cell Polyomavirus Detected by an Innovative Immunological Assay With Mimotopes in Healthy Subjects

Molecular epidemiology and phylodynamics of goose haemorrhagic polyomavirus

Contact Info

Product

Resources

About