Mechanisms of oxidative stress caused by CuO nanoparticles to membranes of the bacterium Streptomyces coelicolor M145

Liu, Xiaomei; Tang, Jingchun; Wang, Lan; Giesy, John P.

doi:10.1016/j.ecoenv.2018.04.007

Cited by 35 publications

(18 citation statements)

References 36 publications

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“…A significant increase in ROS and associated decrease in cell viability in a dose dependent manner, indicates that ROS could have contributed to cell membrane leakage and inflammation, resulting in cell-cycle arrest and subsequent cell death through generation of oxidative stress 65 . Our results are in accordance with previous studies that treatment of cells with Cu 2 O NPs can cause cytotoxicity and DNA damage to biomolecules such as DNA, proteins and lipids through generation of significant amount of ROS induced oxidative stress 52,53 . The mechanism of nanoparticles induced oxidative stress varies among different nanoparticles and the underlying mechanism of ROS production is not clearly understood.…”

Section: Dissolution and Cellular Uptake Of Cusupporting

confidence: 93%

“…To elucidate the other proposed mechanism of toxicity of the Cu 2 O NPs species, an assay measuring cellular ROS generation have been employed on both B. subtilis CN2 and P. aeruginosa CB1 strains. Induction of cellular oxidative stress due to ROS formation has been attributed to be one of the principal bactericidal mechanisms of action of metal nanoparticles 10 , 41 , 52 , 53 . To examine if the toxicity observed in the Cu 2 O NPs studied is related to the ROS induced oxidative stress, the level of cellular oxidative stress triggered by Cu 2 O NPs at increasing dosages (0, 31.25, 62.5, 125 µg/mL) was measured by flow cytometer (FACS) using H 2 DCFDA staining method, which fluoresces in response to ROS inside the cells to fluorescent DCF 54 .…”

Section: Resultsmentioning

confidence: 99%

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Fabrication of monodispersed copper oxide nanoparticles with potential application as antimicrobial agents

Bezza

Tichapondwa

Chirwa

2020

Sci Rep

162

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Cuprous oxide nanoparticles (Cu2O NPs) were fabricated in reverse micellar templates by using lipopeptidal biosurfactant as a stabilizing agent. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive x-ray spectrum (EDX) and UV–Vis analysis were carried out to investigate the morphology, size, composition and stability of the nanoparticles synthesized. The antibacterial activity of the as-synthesized Cu2O NPs was evaluated against Gram-positive B. subtilis CN2 and Gram-negative P. aeruginosa CB1 strains, based on cell viability, zone of inhibition and minimal inhibitory concentration (MIC) indices. The lipopeptide stabilized Cu2O NPs with an ultra-small size of 30 ± 2 nm diameter exhibited potent antimicrobial activity against both Gram-positive and Gram-negative bacteria with a minimum inhibitory concentration of 62.5 µg/mL at pH5. MTT cell viability assay displayed a median inhibition concentration (IC50) of 21.21 μg/L and 18.65 μg/mL for P. aeruginosa and B. subtilis strains respectively. Flow cytometric quantification of intracellular reactive oxygen species (ROS) using 2,7-dichlorodihydrofluorescein diacetate staining revealed a significant ROS generation up to 2.6 to 3.2-fold increase in the cells treated with 62.5 µg/mL Cu2O NPs compared to the untreated controls, demonstrating robust antibacterial activity. The results suggest that lipopeptide biosurfactant stabilized Cu2O NPs could have promising potential for biocompatible bactericidal and therapeutic applications.

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Section: Dissolution and Cellular Uptake Of Cusupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Fabrication of monodispersed copper oxide nanoparticles with potential application as antimicrobial agents

Bezza

Tichapondwa

Chirwa

2020

Sci Rep

162

View full text Add to dashboard Cite

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“…CuO particles of 40 nm, 80 nm, 100 nm, and bulk particles (BP) were purchased from Shanghai Macklin Biochemical Company (Shanghai, China); their characterization is reported in our previous study. They were characterized with an average size of 43.5 ± 2.2 nm, 81.5 ± 4.8 nm, 107.0 ± 5.5 nm, and 1329.0 ± 66.9 nm; and an average aggregate size of 998.0 ± 59.6 nm, 1021.0 ± 104.5 nm, 1034.0 ± 88.5 nm, and 1720.0 ± 73 nm, respectively, in an organic rich medium 19 .…”

Section: Methodsmentioning

confidence: 97%

“…Cytotoxicity of CuO particles to M145 was determined by LIVE/DEAD Bac-Light bacterial viability kit (L-13152; Invitrogen) after being cultured in YBP medium with various amounts of the particles for 24 h 19 . The cell suspension was centrifuged at 4,000 × g for 10 min and washed three times with 0.9% NaCl.…”

Section: Methodsmentioning

confidence: 99%

Mechanism of CuO nano-particles on stimulating production of actinorhodin in Streptomyces coelicolor by transcriptional analysis

Liu

Tang

Wang

et al. 2019

Sci Rep

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In this research, antibiotic-producing bacteria, Streptomyces coelicolor ( S. coelicolor ) M145, was exposed to copper oxide (CuO) particles to investigate the effects of nano-particles (NPs) on antibiotic production. Results showed that a higher yield of antibiotics was obtained with smaller particle sizes of CuO NPs. When exposed to 10 mg/L of 40 nm CuO NPs, the maximum amount of actinorhodin (ACT) obtained was 2.6 mg/L after 144 h, which was 2.0-fold greater than that of control. However, the process was inhibited when the concentration of CuO NPs was increased to higher than 20 mg/L. Transcriptome analysis showed that all the genes involved in the ACT cluster were significantly up-regulated after exposure to 10 mg/L NPs, which could be the direct cause of the increase of ACT production. Additionally, some genes related to the generation of acetyl-coA were up-regulated. In this way, CuO NPs led to an increase of secondary metabolites. The mechanism related to these changes indicated that nano-particle‒induced ROS and Cu 2+ played synergetic roles in promoting ACT biosynthesis. This is a first report suggesting that CuO NPs had a significant effect on antibiotic production, which will be helpful in understanding the mechanism of antibiotic production in nature.

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“…As with other materials that induce this effect, CuO is associated with oxidative stress responses. 167,168 While some species-specific effects are critical to the upregulation of PAFR, 10 this oxidative stress component may be a major factor in the upregulation pathway.…”

Section: Discussionmentioning

confidence: 99%

Interactions of environmental and therapeutic particles with the airway microenvironment

King¹

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v thanks to Joun "Sylvia" Lee and Chantal Allamargot for their assistance with sample preparation and analysis. I would also like to acknowledge the State Hygienic Lab at the University of Iowa for their assistance in quantifying uptake of metal nanoparticles and for trace metals analysis. I would like to thank Brian Wels specifically for his assistance in this. The research presented in chapter 2 was partially supported by the

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Mechanisms of oxidative stress caused by CuO nanoparticles to membranes of the bacterium Streptomyces coelicolor M145

Cited by 35 publications

References 36 publications

Fabrication of monodispersed copper oxide nanoparticles with potential application as antimicrobial agents

Fabrication of monodispersed copper oxide nanoparticles with potential application as antimicrobial agents

Mechanism of CuO nano-particles on stimulating production of actinorhodin in Streptomyces coelicolor by transcriptional analysis

Interactions of environmental and therapeutic particles with the airway microenvironment

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