Gut microbiota recovery and immune response in ampicillin-treated mice

Castro‐Mejía, Josué L.; Jakesevic, Maja; Fabricius, Niels F.; Krych, Łukasz; Nielsen, Dennis Sandris; Kot, Witold; Bendtsen, Katja Maria; Vogensen, Finn K.; Hansen, Camilla Hartmann Friis; Hansen, Axel Kornerup

doi:10.1016/j.rvsc.2018.03.013

Cited by 10 publications

(11 citation statements)

References 40 publications

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“…Antibiotic treatment reduces gene expressions of acute inflammation 64 . It does not seem to have acute or long term effects on expression on the level of CD4, CD8 and FoxP3 positive cells 65 , but it may reduce the activation of CD4 positive T cells in the tonsils 66 . MM transplantation from healthy to IBD induced mice increases levels of FoxP3 positive cells 67 .…”

Section: Discussionmentioning

confidence: 99%

Human microbiota-transplanted C57BL/6 mice and offspring display reduced establishment of key bacteria and reduced immune stimulation compared to mouse microbiota-transplantation

Lundberg

Toft²,

Metzdorff

et al. 2020

Sci Rep

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Transplantation of germ-free (GF) mice with microbiota from mice or humans stimulates the intestinal immune system in disparate ways. We transplanted a human microbiota into GF C57BL/6 mice and a murine C57BL/6 microbiota into GF C57BL/6 mice and Swiss-Webster (SW) mice. Mice were bred to produce an offspring generation. 56% of the Operational Taxonomic Units (OTUs) present in the human donor microbiota established in the recipient mice, whereas 81% of the C57BL/6 OTUs established in the recipient C57BL/6 and SW mice. Anti-inflammatory bacteria such as Faecalibacterium and Bifidobacterium from humans were not transferred to mice. Expression of immune-related intestinal genes was lower in human microbiota-mice and not different between parent and offspring generation. Expression of intestinal barrier-related genes was slightly higher in human microbiota-mice. Cytokines and chemokines measured in plasma were differentially present in human and mouse microbiota-mice. Minor differences in microbiota and gene expression were found between transplanted mice of different genetics. It is concluded that important immune-regulating bacteria are lost when transplanting microbiota from humans to C57BL/6 mice, and that the established human microbiota is a weak stimulator of the murine immune system. The results are important for study design considerations in microbiota transplantation studies involving immunological parameters. The gut microbiota is an important component of human health. For studying its role in health and disease, aiming for the development of microbiota-targeting therapeutics, food products and ingredients, mice transplanted with human microbiotas (HMs) have been described and applied for several decades 1-6 , although concerns pertaining to limitations of this model system have been raised 7-10. Often such models are only studied on their phenotypic expression and the microbiota is not characterized, or the opposite is the case 8. Laboratory rodents are routinely raised in specific pathogen free (SPF) barrier facilities strictly protected from their wild conspecifics. For many years the microbial starting point for many rodent breeding colonies has been the Altered Schaedler Flora (ASF) or variants thereof. ASF consists of eight specific bacterial strains originating from conventional laboratory mice from the 1960's and 1970's, and it is therefore considered mouse-specific 11,12. In addition to this, laboratory rodents are exposed to microbes deriving from human staff in the facility, at least if bedding, food, and other materials are sterilized before introduced to the facility. Evolutionary adaptation to the host environment may drive formation of mouse-specific species and strains originally derived from humans, as

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Section: Discussionmentioning

confidence: 99%

Human microbiota-transplanted C57BL/6 mice and offspring display reduced establishment of key bacteria and reduced immune stimulation compared to mouse microbiota-transplantation

Lundberg

Toft²,

Metzdorff

et al. 2020

Sci Rep

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“…At present, the study of fecal microbes in aquatic mammals is relatively unusual [ 38 ]. The fecal microbiome plays an integral physiological role in host health, including the production of short-chain fatty acids, the synthesis of vitamins, the regulation of the immune system and the development of the brain [ 39 , 40 , 41 , 42 ], which is especially crucial for aquatic mammals like YFPs. Therefore, we took advantage of this short-term therapeutic treatment to explore the changes in the fecal microbiomes of YFPs and the most influential factors of intervention.…”

Section: Discussionmentioning

confidence: 99%

Changes in the fecal microbiome of the Yangtze finless porpoise during a short-term therapeutic treatment

You

Ying

et al. 2020

Open Life Sciences

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AbstractThe fecal microbiome is an integral part of aquatic mammals, like an inner organ. But we know very little about this inner organ of the threatened aquatic species, Yangtze finless porpoise (YFP). Four YFPs were placed into a purse seine for skin ulceration treatment, and this opportunity was taken to nurse the animals closer. In particular, we collected the feces of the YFPs before and after the paired healing and therapeutic treatment, along with samples of their fish diet and water habitat, to explore the changes in their fecal microbiome. Firmicutes (20.9–96.1%), Proteobacteria (3.8–78.7%), Actinobacteria (0.1–35.0%) and Tenericutes (0.8–17.1%) were the most dominant phyla present in the feces. The proportion of Proteobacteria and Actinobacteria increased after the treatment. Firmicutes showed a significant decrease, and most potential pathogens were absent, which reflected the administration of ciprofloxacin hydrochloride. Moreover, environmental shifts can also contribute to changes in the fecal microbiome. These results indicate that certain microbial interactions can be affected by environmental shifts, dietary changes and health-care treatments, which can also help maintain the internal environment of YFPs. These findings will inform the future enhanced protection and management of endangered YFPs and other vulnerable aquatic animals.

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“…3 A). Therefore, we tested the effect of pretreatment with the proton-pump inhibitor omeprazole or with ampicillin, which are known to influence gut microbiota and facilitate intestinal colonization by pathogenic bacteria 17 , 18 . Pretreatment with ampicillin but not with omeprazole led to sufficient growth of KPC-KP in stool 3 and 7 days after start of KPC-KP gavage (Fig.…”

Section: Resultsmentioning

confidence: 99%

An animal model of limitation of gut colonization by carbapenemase-producing Klebsiella pneumoniae using rifaximin

Xenofontos

Renieris

Kalogridi

et al. 2022

Sci Rep

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Current knowledge suggests that infection by carbapenem-resistant enterobacteria is preceded by gut colonization. It is hypothesized that colonization is eradicated by non-absorbable antibiotics like rifaximin. We investigated the effect of rifaximin against carbapenem-resistant Klebsiella pneumoniae (CRKP) in vitro and in a mouse model. We studied the in vitro efficacy of rifaximin against 257 CRKP clinical isolates, 188 KPC producers and 69 OXA-48 producers, by minimum inhibitory concentration and time-kill assays. We then developed a model of gut colonization by feeding 30 C57Bl6 mice with 108 cfu of one KPC-KP isolate for 7 days; mice were pre-treated orally with saline, omeprazole or ampicillin. Then, another 60 mice with established KPC-2 gut colonization received orally for 7 consecutive days rifaximin 180 mg/kg dissolved in ethanol and 4% bile or vehicle. On days 0, 3 and 7 stool samples were collected; mice were sacrificed for determination of tissue outgrowth. At a concentration of 1000 μg/ml rifaximin inhibited 84.8% of CRKP isolates. Α 3 × log10 decrease of the starting inoculum was achieved by 100, 250 and 500 μg/ml of rifaximin after 24 h against 25, 55 and 55% of isolates. Pre-treatment with ampicillin was necessary for gut colonization by KPC-KP. Treatment with rifaximin succeeded in reducing KPC-KP load in stool and in the intestine. Rifaximin inhibits at clinically meaningful gut concentrations the majority of CRKP isolates and is efficient against gut colonization by KPC-KP.

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Gut microbiota recovery and immune response in ampicillin-treated mice

Cited by 10 publications

References 40 publications

Human microbiota-transplanted C57BL/6 mice and offspring display reduced establishment of key bacteria and reduced immune stimulation compared to mouse microbiota-transplantation

Human microbiota-transplanted C57BL/6 mice and offspring display reduced establishment of key bacteria and reduced immune stimulation compared to mouse microbiota-transplantation

Changes in the fecal microbiome of the Yangtze finless porpoise during a short-term therapeutic treatment

An animal model of limitation of gut colonization by carbapenemase-producing Klebsiella pneumoniae using rifaximin

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