Silk fibroin-based woven endovascular prosthesis with heparin surface modification

Li, Yi; Li, Gang; Zheng, Zhaozhu; Li, Yuling; Han, Yifan; Kaplan, David L.; Wang, Xiaoqin

doi:10.1007/s10856-018-6055-3

Cited by 17 publications

(13 citation statements)

References 29 publications

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“…Silk-based biomaterials display unique physicochemical and biological properties, while the spinning process changes the microstructure and affects its water solubility and mechanical performance. The SF nanofibers have high specific surface area and porosity, which is conducive to cell adhesion and drug loading (Keten et al, 2010;Liu et al, 2018;Numata et al, 2012;Tao et al, 2017;Wu et al, 2018;Liu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Fabrication and drug release properties of curcumin-loaded silk fibroin nanofibrous membranes

Xie¹,

Yu²,

Zhao³

et al. 2018

Adsorption Science & Technology

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This paper describes a silk fibroin-based nanofibrous membranes loaded with antitumor drugs curcumin and 5-fluorouracil using electrospinning technique. The concentration of curcumin/ 5-fluorouracil in the silk fibroin solution for electrospinning was optimized to be 0.15/0.25, 0.3/0.5, and 0.45/0.75 wt%. The morphology, hydrophilic property, pore size, secondary structure, and antitumor drugs release of nanofibrous membranes were measured. The diameter of nanofibrous membranes ranged about 100-200 nm. The results indicated that electrospinning process did not influence the secondary structure and the drug content of antitumor drugs, and

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Section: Introductionmentioning

confidence: 99%

Fabrication and drug release properties of curcumin-loaded silk fibroin nanofibrous membranes

Xie¹,

Yu²,

Zhao³

et al. 2018

Adsorption Science & Technology

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“…LMWH solution was prepared at a concentration of 1 mg/mL in PBS (0.01 M, pH 7.4), and carboxylic acid groups of LMWH (LMWH-COOH) were activated using NHS groups at a molar ratio of 10:10:1 (EDC: NHS: LMWH-COOH). The reaction was allowed to proceed for 12 h at 37°C with a pH value of 5.7 (Elahi et al, 2014; Liu Z. et al, 2018). The OCS and EDC/NHS activated LMWH were dialysis against the distilled water for 72 h and further lyophilized to obtain a purified material.…”

Section: Methodsmentioning

confidence: 99%

Low-Molecular-Weight Heparin-Functionalized Chitosan-Chondroitin Sulfate Hydrogels for Controlled Release of TGF-β3 and in vitro Neocartilage Formation

et al. 2019

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Repair of hyaline cartilage remains a huge challenge in clinic because of the avascular and aneural characteristics and the paucity of endogenous repair cells. Recently, tissue engineering technique, possessing unique capacity of repairing large tissue defects, avoiding donor complications and two-stage invasive surgical procedures, has been developed a promising therapeutic strategy for cartilage injury. In this study, we incorporated low-molecular-weight heparin (LMWH) into carboxymethyl chitosan-oxidized chondroitin sulfate (CMC-OCS) hydrogel for loading transforming growth factor-β3 (TGF-β3) as matrix of peripheral blood mesenchymal stem cells (PB-MSCs) to construct tissue-engineered cartilage. Meanwhile, three control hydrogels with or without LMWH and/or TGF-β3 were also prepared. The gelling time, microstructures, mechanical properties, degradation rate, cytotoxicity, and the release of TGF-β3 of different hydrogels were investigated. In vitro experiments evaluated the tri-lineage differentiation potential of PB-MSCs, combined with the proliferation, distribution, viability, morphology, and chondrogenic differentiation. Compared with non-LMWH-hydrogels, LMWH-hydrogels (LMWH-CMC-OCS-TGF-β3) have shorter gelling time, higher mechanical strength, slower degradation rate and more stable and lasting release of TGF-β3. After two weeks of culture in vitro, expression of cartilage-specific genes collagen type-2 (COL-2) and aggrecan (AGC), and secretion of glycosaminoglycan (GAG), and COL-2 proteins in LMWH-CMC-OCS-TGF-β3 group were significantly higher than those in other groups. COL-2 immunofluorescence staining showed that the proportion of COL-2 positive cells and immunofluorescence intensity in LMWH-CMC-OCS-TGF-β3 hydrogel were significantly higher than those in other groups. The LMWH-CMC-OCS-TGF-β3 hydrogel can slowly release TGF-β3 in a long term, and meanwhile the hydrogel can provide a biocompatible microenvironment for the growth and chondrogenic differentiation of PB-MSCs. Thus, LMWH functionalized CMC-OCS hydrogels proposed in this work will be beneficial for constructing functional scaffolds for tissue-engineered cartilage.

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“…As previous research had proven that pure SF has excellent blood compatibility, we did not test the pure SF experimental group. 53 Figure 6(a) showed representative images. The hemolysis rates of the PCL/SF scaffolds with different ratios (1: 10, 1: 5, 1: 1) were 2.94%, 0.75%, and 1.18%, respectively (Figure 6(b)).…”

Section: In Vitro Biocompatibility Evaluationmentioning

confidence: 99%

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

Liu

Chen

et al. 2020

Journal of Bioactive and Compatible Polymers

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Long-distance (⩾10 mm) arterial vascular defect injury was a massive challenge affecting human health. Compared with autologous transplantation, tissue-engineered scaffolds such as biocompatible silk fibroin (SF) scaffolds have been developed because they exhibit equivalent functional repair effects without adverse reactions. However, its mechanical strength and structural stability needed to be further improved to match the longer repair cycle of blood vessels while maintaining the original biological safety. Hence, we designed and prepared SF and hydrophobic polycaprolactone (PCL) composite microfibers by an improving electrospinning method. It was found that when the weight ratio of PCL to SF was 1: 1, a microfiber scaffold with high strength (6.16 N) and minimum degradability can be obtained. More importantly, compared with natural silk fibroin, the novel composite microfiber scaffolds can slightly inhibit cell infiltration and inflammation through co-culture with HUVECs in vitro and rabbit back transplantation in vivo. Furthermore, the fabricated scaffolds also demonstrated excellent structural stability in vivo because of the well-organized PCL doping in the structure. All these results indicated that the novel PCL/SF composite microfiber scaffolds were promising candidates for vascular tissue engineering applications.

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Silk fibroin-based woven endovascular prosthesis with heparin surface modification

Cited by 17 publications

References 29 publications

Fabrication and drug release properties of curcumin-loaded silk fibroin nanofibrous membranes

Fabrication and drug release properties of curcumin-loaded silk fibroin nanofibrous membranes

Low-Molecular-Weight Heparin-Functionalized Chitosan-Chondroitin Sulfate Hydrogels for Controlled Release of TGF-β3 and in vitro Neocartilage Formation

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

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