Author Correction: Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration

Unsain, Nicolás; Bordenave, Martín Diego; Martínez, Gaby F.; Jalil, Sami; Bilderling, Catalina von; Barabas, Federico Martín; Masullo, Luciano A.; Johnstone, Alexandra M.; Barker, Philip A.; Bisbal, Mariano; Stefani, Fernando D.; Cáceres, Alfredo

doi:10.1038/s41598-018-23781-w

Cited by 2 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(A) The MPS abundance and organization in different domains of a neuron, from being robust and well organized in the axon initial segment to being completely absent in the cell soma. (B) Axon shafts from sensory neurons in culture, stained against βII-spectrin and imaged by Stimulated Emission Depletion microscopy (STED) (Unsain et al, 2018 ), reveals the MPS. Scale bar 1 μm.…”

Section: Organization Of the Membrane-associated Periodic Skeletonmentioning

confidence: 99%

“…Over time, the periodical organization spreads distally, and by 12 days in vitro (DIV) the MPS can be found along the entire axonal length (Zhong et al, 2014 ). This proximal to distal developmental pattern awaits confirmation using unbiased sampling approaches and quantitative analysis of the MPS (Barabas et al, 2017 ; Unsain et al, 2018 ). Interestingly, the first proteins to show such a periodic arrangement during axonal growth are F-actin and βII-spectrin.…”

Section: Organization Of the Membrane-associated Periodic Skeletonmentioning

confidence: 99%

“…Whether this irregularity reflects a meaningful biological design remains to be established, and highlights the need to determine MPS abundance (fraction of an axon/dendrite with MPS) and regularity in an unbiased and quantitative manner. Different approaches have been used to tackle these challenges, such as quantifying autocorrelation amplitudes (Zhong et al, 2014 ), sinusoid fit to selected regions (Leterrier et al, 2015 ) and computing the two-dimensional Pearson correlation against a predefined periodic pattern (Barabas et al, 2017 ; Unsain et al, 2018 ). Also, for an unbiased and high volume sampling of neurites, an open-source software was developed that integrates batch analysis, using an automated and unbiased interrogation of neurite segments by two-dimensional Pearson correlation against a modeled MPS (Barabas et al, 2017 ).…”

Section: Organization Of the Membrane-associated Periodic Skeletonmentioning

confidence: 99%

“…Under this condition, the axonal MPS shows a significant decrease in abundance during most of the degeneration period, and is fully dismantled in fragmenting axons. Interestingly, acute pharmacological stabilization of F-actin prevents both MPS loss and axonal fragmentation (Unsain et al, 2018 ). Taken together, these studies suggest that the MPS is not required to keep axon integrity under basal conditions, but instead it might be necessary for coping with physical stress or degenerative triggers.…”

Section: The Mps and Axonal Stabilitymentioning

confidence: 99%

See 3 more Smart Citations

The Actin/Spectrin Membrane-Associated Periodic Skeleton in Neurons

Unsain

Stefani

Cáceres

2018

Front. Synaptic Neurosci.

Self Cite

View full text Add to dashboard Cite

Neurons are the most asymmetric cell types, with their axons commonly extending over lengths that are thousand times longer than the diameter of the cell soma. Fluorescence nanoscopy has recently unveiled that actin, spectrin and accompanying proteins form a membrane-associated periodic skeleton (MPS) that is ubiquitously present in mature axons from all neuronal types evaluated so far. The MPS is a regular supramolecular protein structure consisting of actin “rings” separated by spectrin tetramer “spacers”. Although the MPS is best organized in axons, it is also present in dendrites, dendritic spine necks and thin cellular extensions of non-neuronal cells such as oligodendrocytes and microglia. The unique organization of the actin/spectrin skeleton has raised the hypothesis that it might serve to support the extreme physical and structural conditions that axons must resist during the lifespan of an organism. Another plausible function of the MPS consists of membrane compartmentalization and subsequent organization of protein domains. This review focuses on what we know so far about the structure of the MPS in different neuronal subdomains, its dynamics and the emerging evidence of its impact in axonal biology.

show abstract

Section: Organization Of the Membrane-associated Periodic Skeletonmentioning

confidence: 99%

Section: Organization Of the Membrane-associated Periodic Skeletonmentioning

confidence: 99%

Section: Organization Of the Membrane-associated Periodic Skeletonmentioning

confidence: 99%

Section: The Mps and Axonal Stabilitymentioning

confidence: 99%

See 2 more Smart Citations

The Actin/Spectrin Membrane-Associated Periodic Skeleton in Neurons

Unsain

Stefani

Cáceres

2018

Front. Synaptic Neurosci.

Self Cite

View full text Add to dashboard Cite

show abstract

Dual spatio-temporal regulation of axon growth and microtubule dynamics by RhoA signaling pathways

Wojnacki

Quassollo

Bordenave

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

RhoA plays a crucial role in neuronal polarization, where its action restraining axon outgrowth has been thoroughly studied. We now report that RhoA has not only inhibitory but also a stimulatory effect on axon development depending on when and where exerts its action and the downstream effectors involved. In cultured hippocampal neurons, FRET imaging revealed that RhoA activity selectively localizes in growth cones of undifferentiated neurites, while in developing axons it displays a biphasic pattern, being low in nascent axons and high in elongating ones. RhoA-Rho kinase (ROCK) signaling prevents axon initiation but has no effect on elongation, while formin inhibition reduces axon extension without significantly altering initial outgrowth. Besides, RhoAmDia promotes axon elongation by stimulating growth cone microtubule stability and assembly, as opposed to RhoA-ROCK that restrains growth cone microtubule assembly and protrusion. Finally, we show that similar mechanisms might operate during axonal regeneration, with RhoAROCK slowing axon regrowth after axotomy and RhoA-mDia favoring extension of regenerated axons.

show abstract

Author Correction: Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration

Abstract: which were incorrectly given as Jalil Sami and Bisbal Mariano respectively. This has now been corrected in the PDF and HTML versions of the Article, and in the accompanying supplementary material.

Cited by 2 publications

References 0 publications

The Actin/Spectrin Membrane-Associated Periodic Skeleton in Neurons

The Actin/Spectrin Membrane-Associated Periodic Skeleton in Neurons

Dual spatio-temporal regulation of axon growth and microtubule dynamics by RhoA signaling pathways

Contact Info

Product

Resources

About