Molecular cloning and expression of FGF2 gene in pre‐implantation developmental stages of in vitro‐produced sheep embryos

Mor, Avantika; Mondal, Sukanta; Reddy, I. J.; Nandi, S.; Gupta, P. S. P.

doi:10.1111/rda.13182

Cited by 10 publications

(3 citation statements)

References 41 publications

(59 reference statements)

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“…In addition, DNMT1 maintains the methylation pro le of genes during cell division, and DNMT1 is associated with postimplantation mortality in sheep [48]. FGF2 is a member of the FGF family that mediates trophoblast activities and regulates embryonic development in various species [49]. This result is in line with our data.…”

Section: Discussionsupporting

confidence: 88%

A single-cell atlas of mongolia sheep early embryo

Guo

Guang

et al. 2023

Preprint

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Background The establishment of different cell types during organogenesis in mouse early embryos has been well studied. However, cell type differentiation and its molecular mechanisms in early embryo development in Mongolian sheep are not well understood. Results In this study, we present the first comprehensive single-cell transcriptomic characterization of Ujumqin sheep and Hulunbuir short-tailed sheep at E16. Thirteen major cell types were identified in Ujumqin sheep at E16, and 8 major cell types were identified in Hulunbuir short-tailed sheep at E16. Functional enrichment analysis showed that several pathways were significantly enriched in the notochord cluster, namely, the TGF-beta signaling pathway, Hippo signaling pathway, platelet activation pathway, riboflavin metabolism pathway, Wnt signaling pathway, regulation of actin cytoskeleton, and insulin signaling pathway. In contrast, glutathione metabolism, glyoxylate and dicarboxylate metabolism, citrate cycle, thyroid hormone synthesis, pyruvate metabolism, cysteine and methionine metabolism, thermogenesis, and the VEGF signaling pathway were significantly enriched in the spinal cord cluster. Steroid biosynthesis, riboflavin metabolism, cell cycle, Hippo signaling, Hedgehog signaling, FOXO signaling, JAK-STAT signaling, and Wnt signaling were significantly enriched in the paraxial mesoderm cluster. The notochord cluster, spinal cord cluster and paraxial mesoderm cluster were all strongly related to tail development. Pseudotime analysis demonstrated that mesenchyme can contribute to the notochord in Ujumqin sheep. Further molecular assays showed that the Hippo signaling pathway was enriched in Ujumqin sheep. Conclusion This comprehensive single-cell map reveals the contributions of a previously unrecognized signaling pathway and contributes to our understanding of the mechanism of tail development in short-tailed sheep.

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Section: Discussionsupporting

confidence: 88%

A single-cell atlas of mongolia sheep early embryo

Guo

Guang

et al. 2023

Preprint

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“…In addition, DNMT1 maintains the methylation profile of genes during cell division; DNMT1 is associated with post-implantation mortality in sheep [ 48 ]. FGF2 is a member of the FGF family that mediates trophoblast activity and regulates embryonic development in various species [ 49 ]. This result is in line with our data.…”

Section: Discussionmentioning

confidence: 99%

A Single-Cell Atlas of an Early Mongolian Sheep Embryo

He,

Guo,

Yang

et al. 2023

Veterinary Sciences

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Cell types have been established during organogenesis based on early mouse embryos. However, our understanding of cell types and molecular mechanisms in the early embryo development of Mongolian sheep has been hampered. This study presents the first comprehensive single-cell transcriptomic characterization at E16 in Ujumqin sheep and Hulunbuir short-tailed sheep. Thirteen major cell types were identified at E16 in Ujumqin sheep, and eight major cell types were identified at E16 in Hulunbuir short-tailed sheep. Function enrichment analysis showed that several pathways were significantly enriched in the TGF-beta signaling pathway, the Hippo signaling pathway, the platelet activation pathway, the riboflavin metabolism pathway, the Wnt signaling pathway, regulation of the actin cytoskeleton, and the insulin signaling pathway in the notochord cluster. Glutathione metabolism, glyoxylate, and dicarboxylate metabolism, the citrate cycle, thyroid hormone synthesis, pyruvate metabolism, cysteine and methionine metabolism, thermogenesis, and the VEGF signaling pathway were significantly enriched in the spinal cord cluster. Steroid biosynthesis, riboflavin metabolism, the cell cycle, the Hippo signaling pathway, the Hedgehog signaling pathway, the FoxO signaling pathway, the JAK-STAT signaling pathway, and the Wnt signaling pathway were significantly enriched in the paraxial mesoderm cluster. The notochord cluster, spinal cord cluster, and paraxial mesoderm cluster were found to be highly associated with tail development. Pseudo-time analysis demonstrated that the mesenchyme can translate to the notochord in Ujumqin sheep. Molecular assays revealed that the Hippo signaling pathway was enriched in Ujumqin sheep. This comprehensive single-cell map revealed previously unrecognized signaling pathways that will further our understanding of the mechanism of short-tailed sheep formation.

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“…These data suggest that the expansion of TE in early development depends on FGF in mice, and that this expansion, which is dependent on FGF, occurs more in late stages in humans. The actions of FGF family members begin in the initial steps of reproduction mechanisms, therefore, FGF-10 expression has been detected in theca cells and ovarian stromal cells in humans [ 88 ], and FGF-2 and FGF-10 have been shown to increase the survival and proliferation of cumulus cells, increasing blastocyst development rates in bovine, sheep, and yak, in in vitro cultures [ 89 , 90 , 91 , 92 ]. Another family member, FGF-18, seems to regulate steroidogenesis in the fetal ovary [ 93 ].…”

Section: Growth Factors and Early Developmentmentioning

confidence: 99%

Pluripotency and Growth Factors in Early Embryonic Development of Mammals: A Comparative Approach

Llobat

2021

Veterinary Sciences

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The regulation of early events in mammalian embryonic development is a complex process. In the early stages, pluripotency, cellular differentiation, and growth should occur at specific times and these events are regulated by different genes that are expressed at specific times and locations. The genes related to pluripotency and cellular differentiation, and growth factors that determine successful embryonic development are different (or differentially expressed) among mammalian species. Some genes are fundamental for controlling pluripotency in some species but less fundamental in others, for example, Oct4 is particularly relevant in bovine early embryonic development, whereas Oct4 inhibition does not affect ovine early embryonic development. In addition, some mechanisms that regulate cellular differentiation do not seem to be clear or evolutionarily conserved. After cellular differentiation, growth factors are relevant in early development, and their effects also differ among species, for example, insulin-like growth factor improves the blastocyst development rate in some species but does not have the same effect in mice. Some growth factors influence genes related to pluripotency, and therefore, their role in early embryo development is not limited to cell growth but could also involve the earliest stages of development. In this review, we summarize the differences among mammalian species regarding the regulation of pluripotency, cellular differentiation, and growth factors in the early stages of embryonic development.

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Molecular cloning and expression of FGF2 gene in pre‐implantation developmental stages of in vitro‐produced sheep embryos

Cited by 10 publications

References 41 publications

A single-cell atlas of mongolia sheep early embryo

A single-cell atlas of mongolia sheep early embryo

A Single-Cell Atlas of an Early Mongolian Sheep Embryo

Pluripotency and Growth Factors in Early Embryonic Development of Mammals: A Comparative Approach

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