The peptidyl-prolyl cis-trans isomerase activity of the wheat cyclophilin, TaCypA-1, is essential for inducing thermotolerance in Escherichia coli

Abstract

Growth at high temperatures is one of the desired features for industrial applications of microbes, as it results in decrease in contamination and enhanced solubility of certain substrates. In this study, it is demonstrated that heterologous expression of a wheat cyclophilin, TaCypA-1, confers thermotolerance to Escherichia coli. The TaCypA-1 possesses peptidyl-prolyl cis-trans isomerase (PPIase) activity that catalyses cis to trans isomerization of the peptidyl prolyl bonds, a rate limiting step in protein folding. Expression of deleted mutants of TaCypA-1, that lacked PPIase activity, resulted in abrogation of thermotolerance, providing the first evidence that this activity plays a key role in stress tolerance of cells and can be exploited for industrial applications. Further, we also demonstrate that TaCypA-1 interacts with calmodulin (CaM), and the CaM-binding domain is localized to amino acid residues 51–71 in the N-terminus region.

Proteomics provides insights into the inhibition of Chinese hamster V79 cell proliferation in the deep underground environment

“…In contrast, peptidyl-prolyl cis-trans isomerase (PPIase) and its subunits were up-regulated in V79 cells cultured in the DUGL. PPIase represents a rate limiting step in protein folding, catalyses cis to trans isomerization of peptidyl prolyl bonds [37,38] and is involved in many biological processes. PPIase has been implicated in stress tolerance in wheat; therefore, PPIase activity in V79 cells may have been responsible for enhanced protection against decreased background radiation [37].…”

Genetic components of root architecture and anatomy adjustments to water‐deficit stress in spring barley

“…SNP ( BOPA2_12_11044) at 86 cM on 7H associated with the observed variations in the number of nodal roots is physically located in the domain of PPIB (HORVU7Hr1G095720). PPIB is catalyzed by LATERAL ROOTLESS2 to regulate root gravitropism, lateral root development, and induce thermo‐tolerance (Xi et al, ; Kaur et al, ). Root gravitropism is a physiological drought response that redirects root growth by gravitational pull toward the down water sources via auxin transport.…”

“…SNP (BOPA2_12_11044) at 86 cM on 7H associated with the observed variations in the number of nodal roots is physically located in the domain of PPIB (HORVU7Hr1G095720). PPIB is catalyzed by LAT-ERAL ROOTLESS2 to regulate root gravitropism, lateral root development, and induce thermo-tolerance (Xi et al, 2016;Kaur et al, 2016).…”

Plant Cyclophilins: Multifaceted Proteins With Versatile Roles

“…Though molecular processes underlying the cyclophilininduced stress tolerance are not fully understood for the majority of the cyclophilins, prevention of protein aggregation, as reported for GjCYP-1, may be one of the protective mechanisms (Cho et al, 2005). The heat stress tolerance in E. coli that overexpressed a redox-regulated wheat cytosolic cyclophilin, TaCYPA-1, was however attributed to its PPIase activity (Kaur et al, 2016(Kaur et al, , 2017. Since the redox status of plants undergoes reversible changes under stress conditions (Jubany-Mari et al, 2010), application of a redox-sensing GFP (c-roGFP1) for real-time monitoring of cytosol redox status (Brossa et al, 2013) is needed to explore the role of TaCYPA-1 in the maintenance of redox Saito et al, 1999a;Grebe et al, 2000 AtCYP18-1 NC_003070.9 ND Heat Sakuma et al, 2006 AtCYP18-3 (ROC1) NC_003075 -Salt He et al, 2004 AtCYP20-2 NC_003076.8 PPIase activity High irradiance Romano et al, 2004a;Edvardsson et al, 2007 CYP38 NM_111014.4 PPIase inactive High light Shapiguzov et al, 2006;Wang et al, 2015 Brassica rapa BrROC1 BrROC2 NC_024800.1 KJ173687 ND Cold, heat, dehydration, mannitol, salinity, light Yan et al, 2018 Cajanus cajan CcCYP GU444041 PPIase activity Salt, drought Sekhar et al, 2010 Capsicum annuum CACYP1 AF291180 ND Salicylic acid, MeJA, ethylene and pathogen Kong et al, 2001 Digitalis lanata DLCYP18.0/ DLCYP18.1 Y08320.1 PPIase activity Abscisic acid, sorbitol Küllertz et al, 1999 DLCYP Y08320 ND PbN0 3 and salt Scholze et al, 1999 Gossypium hirsutum GhCYP1 GQ292530.1 ND Salt stress, biotic stress Zhu et al, 2011 Nicotiana tabacum Cyclophilin-like protein EF495223.1 -Induced by low nitrogen Yang et al, 2013 Oryza sativa OsCyp2 EF576508 PPIase activity Salinity, high temperature, osmotic stress and oxidative stress Kumari et al, 2009Kumari et al, , 2015Ruan et al, 2011 OsCYP18 Kim et al, 2010 The nomenclature used below is as given in literature.…”