Report of
            <i>erm</i>
            (B)
            <sup>+</sup>
            Campylobacter jejuni in the United States

Chen, Jessica C.; Tagg, Kaitlin A.; Joung, Yoo Jin; Bennett, Christy; Watkins, Louise Francois; Eikmeier, Dana; Folster, Jason P.

doi:10.1128/aac.02615-17

Cited by 28 publications

(18 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resistance to erythromycin is commonly mediated by mutations in 23S rRNA, especially A2075G [36,48]. C. coli is more often resistant to macrolides than C. jejuni, and the ermB gene has been shown to mediate resistance to erythromycin in C. coli isolates of animal origin [49,50], but recently ermB was also identified in a clinical C. jejuni isolate [51]. In this study, none of the isolates had the ermB gene.…”

Section: Discussionmentioning

confidence: 57%

Prediction of antimicrobial resistance in clinical Campylobacter jejuni isolates from whole-genome sequencing data

Dahl

Joensen

Østerlund

et al. 2020

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

Campylobacter jejuni is recognised as the leading cause of bacterial gastroenteritis in industrialised countries. Although the majority of Campylobacter infections are self-limiting, antimicrobial treatment is necessary in severe cases. Therefore, the development of antimicrobial resistance (AMR) in Campylobacter is a growing public health challenge and surveillance of AMR is important for bacterial disease control. The aim of this study was to predict antimicrobial resistance in C. jejuni from whole-genome sequencing data. A total of 516 clinical C. jejuni isolates collected between 2014 and 2017 were subjected to WGS. Resistance phenotypes were determined by standard broth dilution, categorising isolates as either susceptible or resistant based on epidemiological cutoffs for six antimicrobials: ciprofloxacin, nalidixic acid, erythromycin, gentamicin, streptomycin, and tetracycline. Resistance genotypes were identified using an in-house database containing reference genes with known point mutations and the presence of resistance genes was determined using the ResFinder database and four bioinformatical methods (modified KMA, ABRicate, ARIBA, and ResFinder Batch Upload). We identified seven resistance genes including tet(O), tet(O/32/O), ant(6)-Ia, aph(2″)-If, blaOXA, aph(3′)-III, and cat as well as mutations in three genes: gyrA, 23S rRNA, and rpsL. There was a high correlation between phenotypic resistance and the presence of known resistance genes and/or point mutations. A correlation above 98% was seen for all antimicrobials except streptomycin with a correlation of 92%. In conclusion, we found that WGS can predict antimicrobial resistance with a high degree of accuracy and have the potential to be a powerful tool for AMR surveillance.

show abstract

Section: Discussionmentioning

confidence: 57%

Prediction of antimicrobial resistance in clinical Campylobacter jejuni isolates from whole-genome sequencing data

Dahl

Joensen

Østerlund

et al. 2020

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

show abstract

“…It is noteworthy that, since the first report of erm(B) in Campylobacter (14), novel genetic environments have continuously been described. Analysis of all published studies of erm(B) in Campylobacter (14,15,17,18,(22)(23)(24)(25) shows that type VI and type III are the predominant MDRGI types in this genus, with both types consistently being represented in isolates from humans and animals. This finding suggests that these two MDRGI types are widely disseminated among Campylobacter isolates (Table 2).…”

Section: Characterization Of Erm(b)-carrying C Jejuni Isolatesmentioning

confidence: 99%

“…Data regarding erm(B)-positive isolates recorded in previous studies were also obtained, to allow composite analysis. Collected data included prevalence, PFGE profiling, and MDRGI clustering information (14,15,17,18,(22)(23)(24)(25).…”

Section: Characterization Of Erm(b)-carrying C Jejuni Isolatesmentioning

confidence: 99%

“…Identification of MDRGIs and whole-genome sequencing. Except for type IX (17), which may have been located within a plasmid, all classes of erm(B)-carrying MDRGIs in Campylobacter (types I to VIII) have been chromosomally located. PCR primers, the sequences of which are listed in Table S2, were designed to amplify all type I to VIII MDRGIs.…”

Section: Campylobacter Isolates and Detectionmentioning

confidence: 99%

“…Interestingly, worldwide macrolide resistance rates for C. jejuni isolates are much lower than those for C. coli (16). Similarly, while nine classes of erm(B)-harboring MDRGIs have been detected in Campylobacter strains from China, Spain, and the United States, only two of those classes (types VII and IX) were detected in C. jejuni, with the remaining seven classes all being identified in C. coli isolates (15,17,18). Of note, novel erm(B) genetic environments are continually emerging in Campylobacter.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Emerging erm (B)-Mediated Macrolide Resistance Associated with Novel Multidrug Resistance Genomic Islands in Campylobacter

Liu

et al. 2019

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The rapid dissemination of the macrolide resistance gene erm(B) will likely compromise the efficacy of macrolides as the treatment of choice for campylobacteriosis. More importantly, erm(B) is always associated with several multidrug resistance genomic islands (MDRGIs), which confer resistance to multiple other antimicrobials. Continuous monitoring of the emergence of erm(B) and analysis of its associated genetic environments are crucial for our understanding of macrolide resistance in Campylobacter. In this study, 290 Campylobacter isolates (216 Campylobacter coli isolates and 74 Campylobacter jejuni isolates) were obtained from 1,039 fecal samples collected in 2016 from pigs and chickens from three regions of China (344 samples from Guangdong, 335 samples from Shanghai, and 360 samples from Shandong). Overall, 74 isolates (72 C. coli isolates and 2 C. jejuni isolates) were PCR positive for erm (B). Combined with data from previous years, we observed a trend of increasing prevalence of erm(B) in C. coli. Pulsed-field gel electrophoresis analyses suggested that both clonal expansion and horizontal transmission were involved in the dissemination of erm(B) in C. coli, and three novel types of erm(B)-associated MDRGIs were identified among the isolates. Furthermore, 2 erm(B)-harboring C. jejuni isolates also contained an aminoglycoside resistance genomic island and a multidrug-resistance-enhancing efflux pump, encoded by RE-cmeABC. Antimicrobial susceptibility testing showed that most of the isolates were resistant to all clinically important antimicrobial agents used for the treatment of campylobacteriosis. These findings suggest that the increasing prevalence of erm(B)-associated MDRGIs might further limit treatment options for campylobacteriosis.

show abstract

Natural Competence and Horizontal Gene Transfer in Campylobacter

Golz

Stingl

2021

Current Topics in Microbiology and Immunology

View full text Add to dashboard Cite

Thermophilic Campylobacter, in particular Campylobacter jejuni, C. coli and C. lari are the main relevant Campylobacter species for human infections. Due to their high capacity of genetic exchange by horizontal gene transfer (HGT), rapid adaptation to changing environmental and host conditions contribute to successful spreading and persistence of these foodborne pathogens. However, extensive HGT can exert dangerous side effects for the bacterium, such as the incorporation of gene fragments leading to disturbed gene functions. Here we discuss mechanisms of HGT, notably natural transformation, conjugation and bacteriophage transduction and limiting regulatory strategies of gene transfer. In particular, we summarize the current knowledge on how the DNA macromolecule is exchanged between single cells. Mechanisms to stimulate and to limit HGT obviously coevolved and maintained an optimal balance. Chromosomal rearrangements and incorporation of harmful mutations are risk factors for survival and can result in drastic loss of fitness. In Campylobacter, the restricted recognition and preferential uptake of free DNA from relatives are mediated by a short methylated DNA pattern and not by a classical DNA uptake sequence as found in other bacteria. A class two CRISPR-Cas system is present but also other DNases and restriction–modification systems appear to be important for Campylobacter genome integrity. Several lytic and integrated bacteriophages have been identified, which contribute to genome diversity. Furthermore, we focus on the impact of gene transfer on the spread of antibiotic resistance genes (resistome) and persistence factors. We discuss remaining open questions in the HGT field, supposed to be answered in the future by current technologies like whole-genome sequencing and single-cell approaches.

show abstract

Report of erm (B) ⁺ Campylobacter jejuni in the United States

Cited by 28 publications

References 10 publications

Prediction of antimicrobial resistance in clinical Campylobacter jejuni isolates from whole-genome sequencing data

Prediction of antimicrobial resistance in clinical Campylobacter jejuni isolates from whole-genome sequencing data

Emerging erm (B)-Mediated Macrolide Resistance Associated with Novel Multidrug Resistance Genomic Islands in Campylobacter

Natural Competence and Horizontal Gene Transfer in Campylobacter

Contact Info

Product

Resources

About

Report of erm (B) + Campylobacter jejuni in the United States

Cited by 28 publications

References 10 publications

Prediction of antimicrobial resistance in clinical Campylobacter jejuni isolates from whole-genome sequencing data

Prediction of antimicrobial resistance in clinical Campylobacter jejuni isolates from whole-genome sequencing data

Emerging erm (B)-Mediated Macrolide Resistance Associated with Novel Multidrug Resistance Genomic Islands in Campylobacter

Natural Competence and Horizontal Gene Transfer in Campylobacter

Contact Info

Product

Resources

About

Report of erm (B) ⁺ Campylobacter jejuni in the United States