Anti-cancer effect of toosendanin and its underlying mechanisms

Zhang, Sha; Cao, Liang; Wang, Zongren; Li, Zhe; Ma, Jing

doi:10.1080/10286020.2018.1451516

Cited by 32 publications

(15 citation statements)

References 66 publications

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“…Moreover, Zahang et al [ 71 ] found that sulfur significantly enhanced the tolerance of oil seed rape exposed to Cr stress by activating several detoxification mechanisms such as the ascorbate-glutathione (AsA-GSH) enzyme defense system and GSH production. Kumaresan et al [ 72 ] studied sulfur stress on Arthrospira sp.…”

Section: Resultsmentioning

confidence: 99%

“…GSTs are involved in the detoxification mechanisms, and 15 GST- Data are presented as means ± SD (n = 3) in each column, and for each concentration, means with different letters are significantly different (p < 0.05) like isoenzymes (Cr GSTS) were found in the model species of green alga Chlamydomonas reinhardtii [70]. Moreover, Zahang et al [71] found that sulfur significantly enhanced the tolerance of oil seed rape exposed to Cr stress by activating several detoxification mechanisms such as the ascorbate-glutathione (AsA-GSH) enzyme defense system and GSH production. Kumaresan et al [72] studied sulfur stress on Arthrospira sp.…”

Section: Effect Of Culture Media Composition On Secondary Metabolite Production Of N Linckiamentioning

confidence: 99%

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Enhancement of active ingredients and biological activities of Nostoc linckia biomass cultivated under modified BG-110 medium composition

El-fayoumy

Shanab

Hassan

et al. 2021

Biomass Conv. Bioref.

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The current work aims to investigate the effect of abiotic stresses (nitrogen (N) and sulfur (S) [0.0 g/l, 1.5 g/l, 3 g/l, 6 g/l, and 12 g/l N and 0.0 g/l, 0.07 g/l, 0.15 g/l, 0.3 g/l, and 0.6 g/l S] and their combination [0.3 g/l S + 6 g/l N]) of axenic culture of Nostoc linckia on the production of secondary metabolites which induce different biological activities. Growth rate was measured by dry weight (DW) and optical density (OD) 550 nm . Additionally, phytochemical compounds, defense enzymes as well as antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(ethylbenzthiazoline-6-sulfonic acid) (ABTS ·+ ) radical assays of crude extracts (methylene chloride:methanol, 1:1) were evaluated. Based on antioxidant activity, four promising extracts were subjected to different biological activities such as anticancer, antimicrobial, and antiviral activities. The obtained results revealed that supplementation of external nitrogen source in the form of sodium nitrate was found to increase the total phycobiliprotein content by fivefold. Also, nitrogen depletion provoked significantly highest quantities of phenolic and flavonoid content and this has effects on biological activities of Nostoc linckia . Moreover, 0.3 g/l S was found to be the most effective extract exhibiting a significant increase in antioxidant activity based on DPPH and ABTS assays, respectively (88.18 ± 0.64% and 84.20 ± 1.01%). Furthermore, it recorded anticancer activity against HCT 116 cell line with IC 50 of 155 μg/ml. Moreover, this extract possessed a noticeable antibacterial potency (21.0 ± 1.0 as mm inhibition zone against Staphylococcus aureus and 19.3 ± 0.6 against Streptococcus mutans ). In addition, its antiviral activity against H 5 N 1 virus as a percentage of inhibition was 50% and 63.6% at a concentration of 7 μg/ml and 28 μg/ml, respectively, with cytotoxicity less than 7 μg/μl. GC-MS analysis recorded the presence of bioactive compounds exhibiting different biological activities. Therefore, the obtained results can represent valuable bioactive compounds with variable biological potencies.

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Section: Resultsmentioning

confidence: 99%

Section: Effect Of Culture Media Composition On Secondary Metabolite Production Of N Linckiamentioning

confidence: 99%

Enhancement of active ingredients and biological activities of Nostoc linckia biomass cultivated under modified BG-110 medium composition

El-fayoumy

Shanab

Hassan

et al. 2021

Biomass Conv. Bioref.

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“…Previous studies have reported that TSN inhibits a number of pathways and targets that are crucial to cancer cell survival and proliferation ( 4 ), such as TSN induced apoptosis of cells through the β-catenin and MAPK pathways ( 6 , 5 ). Zhang et al ( 27 ) has previously shown that TSN selectively inhibits U87 and C6 glioma cell proliferation and induces apoptosis through oestrogen receptor-dependent machinery. In order to study the mechanism of TSN-induced apoptosis of human gastric cancer MKN-45 cells, the expression of apoptosis-related genes was examined.…”

Section: Discussionmentioning

confidence: 99%

Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2

Shao

Liu

et al. 2020

Mol Med Rep

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Toosendanin (TSn) is a tetracyclic triterpenoid extracted from Melia toosendan Sieb, et Zucc, which primarily grows in specific areas of china. although toosendanin (TSn) exerts antitumoral effects on various human cancer cells, its influence on gastric cancer (GC) is remains to be elucidated. Micrornas (mirnas/mirs) serve crucial roles in apoptosis and proliferation of cancer cells. mir-23a-3p has been shown to be associated with human GC; however, the specific function of miR-23a-3p in GC remains unclear. Therefore, the present study aimed to elucidate the role of miR-23a-3p in the regulation of GC cell proliferation and apoptosis induced in vitro by TSn treatment. Subsequently, apoptosis-related genes expression levels were quantified by reverse transcription-quantitative Pcr and western blot analysis, respectively, and the target relationship between mir-23a-3p and BCL2 was determined by luciferase reporter gene analysis. additionally, cell proliferation and apoptosis experiments were carried out. The results indicated that TSn inhibited proliferation and induced apoptosis in MKn-45 cells. Moreover, it upregulated the expression of mir-23a-3p. B-cell lymphoma-2 (BCL2) was identified as a potential target gene of mir-23a-3p, which was demonstrated to bind to the 3'-untranslated region of BCL2 mrna, as detected by the luciferase reporter assay. Further studies revealed that BCL2 expression was downregulated following overexpression of mir-23a-3p. in addition, the overexpression of the mir-23a-3p inhibited proliferation, induced G 1 arrest and increased apoptosis in MKn-45 cells. The results of the present study demonstrated that mir-23a-3p inhibited proliferation and induced apoptosis of GC cells, which may be attributable to its direct targeting of BCL2. These results may provide a novel insight into the apoptosis of GC cells, and may lead to investigations into the mechanisms of the effects of TSn.

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“…Studies showed that Szechwan Chinaberry extracts exhibited biological activities in blocking neuromuscular junctions, antibotulism, and affecting efferent nerves, central nervous and the respiratory system [15][16][17] . Recently, several experts and scholars pointed out that Szechwan Chinaberry extracts have antineoplastic effects, including inhibition to hepatocellular carcinoma cells, human leukemia cells and human breast cancer cells, by primarily inducing the apoptosis of relevant cells [18][19][20] . In this paper, the Szechwan Chinaberry extracts were added at 25, 50 and 75 nM concentrations to MCF-7 cells.…”

Section: Effects Of Chinaberry Tree Extract On Proliferation Apoptosmentioning

confidence: 99%

Effects of Chinaberry Tree Extract on Proliferation, Apoptosis, Migration and Cell Cycle Distribution of Human Breast Cancer Cells

Xiao¹,

Chu²,

Hongyan³

et al. 2020

pharmaceutical-sciences

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Jian, et al.: Effects of Chinaberry Tree Extract on Breast Cancer Cells To study the effects of chinaberry tree extract on proliferation, apoptosis, migration and cell cycle distribution of human breast cancer cells, the MCF-7 breast cancer cells obtained were divided into blank group, low concentration group, medium concentration group and high concentration group. Phosphate buffered saline solution and sterile physiological saline were added to the blank group for cell culture. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry were used to detect cell proliferation and apoptosis at different time periods. Transwell assay was used to detect cell migration; flow cytometry was used to detect cell cycle distribution, and intercellular cell adhesion molecule-1, matrix metalloproteinase-2, matrix metalloproteinase-9 were detected by Western blotting. The proliferation rate of MCF-7 breast cancer cells in the low, medium and high concentration group was significantly lower than that in the blank group at different time periods, while the apoptosis rate was significantly higher than that in the blank group, and the effect was more obvious with the increase of concentration (p<0.05). The number of cell migration of MCF-7 breast cancer cells in the low, medium and high concentration group was significantly reduced compared with the blank group, and the cell migration was significantly reduced with the increase of the extract concentration of chinaberry tree (p<0.05). The higher the extract concentration of chinaberry tree, the lower the proportion of MCF-7 cells in G0/G1 and S cycle and the higher the proportion of MCF-7 cells in G2/M cycle (p<0.05). The expressions of intercellular cell adhesion molecule-1, matrix metalloproteinase-2 and matrix metalloproteinase-9 in the low, medium and high concentration groups were significantly lower than those in the blank group, and the expressions of intercellular cell adhesion molecule-1, matrix metalloproteinase-2 and matrix metalloproteinase-9 in the high concentration group were significantly lower than those in the low and medium concentration groups (p<0.05). The extract of chinaberry tree can effectively inhibit the proliferation and migration promote the apoptosis and reduce the growth of breast cancer cells by changing the periodic distribution of cancer cells, and its ability is concentration dependent.

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Anti-cancer effect of toosendanin and its underlying mechanisms

Cited by 32 publications

References 66 publications

Enhancement of active ingredients and biological activities of Nostoc linckia biomass cultivated under modified BG-110 medium composition

Enhancement of active ingredients and biological activities of Nostoc linckia biomass cultivated under modified BG-110 medium composition

Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2

Effects of Chinaberry Tree Extract on Proliferation, Apoptosis, Migration and Cell Cycle Distribution of Human Breast Cancer Cells

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