Induction of Human Adipose-Derived Mesenchymal Stem Cells into Germ Lineage Using Retinoic Acid

Liu, Haihui; Chen, Mingtai; Liu, Lulu; Ren, Shengxiang; Cheng, Panpan; Zhang, Hao

doi:10.1089/cell.2017.0063

Cited by 7 publications

(3 citation statements)

References 30 publications

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“… 65 Human adipose-derived MSCs can also be induced into germ-like cells by RA. 66 RA is an important regulator of meiosis in gametogenesis, and it induces the expression of meiosis markers during spermatogenesis. 67 Growth factors, e.g ., LIF, GDNF, and EGF, contribute to the long-term culture of MSCs, whereas RA promotes germ cell differentiation of MSCs.…”

Section: Derivation Of Male Germ Cells From the Mscsmentioning

confidence: 99%

Generation of male germ cells in vitro from the stem cells

Cui¹,

Chen²,

Wan³

et al. 2023

Asian J Androl

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Infertility has become a serious disease since it affects 10%–15% of couples worldwide, and male infertility contributes to about 50% of the cases. Notably, a significant decrease occurs in the newborn population by 7.82 million in 2020 compared to 2016 in China. As such, it is essential to explore the effective methods of obtaining functional male gametes for restoring male fertility. Stem cells, including embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), spermatogonial stem cells (SSCs), and mesenchymal stem cells (MSCs), possess the abilities of both self-renewal and differentiation into germ cells. Significantly, much progress has recently been achieved in the generation of male germ cells in vitro from various kinds of stem cells under the specified conditions, e.g ., the coculturing with Sertoli cells, three-dimensional culture system, the addition of growth factors and cytokines, and/or the overexpression of germ cell-related genes. In this review, we address the current advance in the derivation of male germ cells in vitro from stem cells based on the studies of the peers and us, and we highlight the perspectives and potential application of stem cell-derived male gametes in reproductive medicine.

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Section: Derivation Of Male Germ Cells From the Mscsmentioning

confidence: 99%

Generation of male germ cells in vitro from the stem cells

Cui¹,

Chen²,

Wan³

et al. 2023

Asian J Androl

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show abstract

“…Successful spermatogenesis was achieved in guinea pigs with busulfan-induced azoospermia following injection of BM MSCs [113]. Allogenic AT MSCs could differentiate into cells similar to spermatogenic epithelium in vitro, providing theoretical and experimental background for clinical use of AT MSCs in treatment of infertility in animals such as guinea pigs with azoospermia [114]. In rats, BM MSCs mitigate the toxic effect of cisplatin on testes at both genetic and molecular levels [113].…”

Section: Adipose Derived Mesenchymal Stem Cells In Treatment Of Non Obstructive Azoospermiamentioning

confidence: 99%

Adipose Derived Mesenchymal Stem Cells Restore Spermatogenesis in Male non Obstructive Azoospermia (Literature Review)

Tamadon

Аскаров

Zhanbyrbekuly

et al. 2021

BMCRM

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“…Many groups have isolated MSCs from the umbilical cord 23–49 and cord blood 23,50–55 . SSEA3+ cells comprise 0.03% to several percent of MSCs cultured from goat skin, human dermal fibroblasts, adipose tissue, and bone marrow 3,20 .…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting

Leng

Sun

Huang³

et al. 2019

Cell Transplant

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Multilineage-differentiating stress-enduring (Muse) cells are a population of pluripotent stage-specific embryonic antigen 3 (SSEA3)+ mesenchymal stem cells first described by Mari Dezawa in 2010. Although some investigators have reported SSEA3+ mesenchymal cells in umbilical cord tissues, none have quantitatively compared SSEA3+ cells isolated from Wharton’s jelly (WJ) and the cord lining (CL) of human umbilical cords (HUCs). We separated WJ and the CL from HUCs, cultured mesenchymal stromal cells (MSCs) isolated from these two tissues with collagenase, and quantified the percentage of SSEA3+ cells over three passages. The first passage had 5.0% ± 4.3% and 5.3% ± 5.1% SSEA3+ cells from WJ and the CL, respectively, but the percentage of SSEA3+ cells decreased significantly ( P < 0.05) between P0 and P2 in the CL group and between P0 and P1 in the WJ group. Magnetic-activated cell sorting (MACS) markedly enriched SSEA3+ cells to 91.4% ± 3.2%. Upon culture of the sorted population, we found that the SSEA3+ percentage ranged from 62.5% to 76.0% in P2–P5 and then declined to 42.0%–54.7% between P6 and P9. At P10, the cultures contained 37.4% SSEA3+ cells. After P10, we resorted the cells and achieved 89.4% SSEA3+ cells in culture. The procedure for MACS-based enrichment of SSEA3+ cells, followed by expansion in culture and a re-enrichment step, allows the isolation of many millions of SSEA3+ cells in relatively pure culture. When cultured, the sorted SSEA3+ cells differentiated into embryoid spheres and survived 4 weeks after transplant into a contused Sprague-Dawley rat spinal cord. The transplanted SSEA3+ cells migrated into the injury area from four injection points around the contusion site and did not produce any tumors. The umbilical cord is an excellent source of fetal Muse cells, and our method allows the practical and efficient isolation and expansion of relatively pure populations of SSEA3+ Muse cells that can be matched by human leukocyte antigen for transplantation in human trials.

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Induction of Human Adipose-Derived Mesenchymal Stem Cells into Germ Lineage Using Retinoic Acid

Cited by 7 publications

References 30 publications

Generation of male germ cells in vitro from the stem cells

Generation of male germ cells in vitro from the stem cells

Adipose Derived Mesenchymal Stem Cells Restore Spermatogenesis in Male non Obstructive Azoospermia (Literature Review)

Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting

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