Correction: A FRET-based biosensor for measuring Gα13 activation in single cells

Mastop, Marieke; Reinhard, Nathalie R.; Zuconelli, Cristiane R.; Terwey, Fenna; Gadella, Theodorus W. J.; Unen, Jakobus van; Adjobo‐Hermans, Merel J. W.; Goedhart, Joachim

doi:10.1371/journal.pone.0195649

Cited by 1 publication

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“…This construct will be referred to as pc-AstR-C. Different FRET biosensors including Gi 1 , Gi 2 , Gi 3 , Gq, Gs, and G 13 , were used to measure the G protein activation. In these biosensors, the Gα subunit is tagged with mTurquise2 and Gγ 2 is tagged with mVenus .…”

Section: Methodsmentioning

confidence: 99%

Structural and Functional Characterization of Allatostatin Receptor Type-C of Thaumetopoea pityocampa, a Potential Target for Next-Generation Pest Control Agents

Shahraki

Işbilir

Doğan

et al. 2021

J. Chem. Inf. Model.

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Insect neuropeptide receptors, including allatostatin receptor type C (AstR-C), a G protein-coupled receptor, are among the potential targets for designing next-generation pesticides that despite their importance in offering a new mode-of-action have been overlooked. Focusing on AstR-C of Thaumetopoea pityocampa, a common pest in Mediterranean countries, by employing resonance energy transfer-based methods, we showed Gαi/o coupling and β-arrestin recruitment of the receptor at sub-nanomolar and nanomolar ranges of the endogenous ligand, AST-C, respectively. Molecular docking and molecular dynamics simulation studies revealed the importance of extracellular loop 2 in AstRC/AST-C interaction, and a combination of in silico and in vitro approaches showed the substantial role of Q271 6.55 in G proteindependent activation of AstR-C possibly via contributing to the flexibility of the receptor's structure. The functional and structural insights obtained on T. pit AstR-C positively assist future efforts in developing environmentally friendly pest control agents that are needed urgently.

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