Molecular characterization of fowl adenovirus group I in commercial broiler chickens in Brazil

Torre, David De la; Parra, Silvana Hipatia Santander; Astolfi-Ferreira, Claudete S.; Ferreira, Antônio José Piantino

doi:10.1007/s13337-018-0430-z

Cited by 19 publications

(19 citation statements)

References 26 publications

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“…The decreased weight gain and gross lesions observed in livers of birds seen in FAdV-11(MOR300315) inoculated SPF chickens were comparable to those obtained in other experiments [ 28 , 44 ] as well as in natural infections with FAdV-11 [ 5 , 6 , 33 , 42 , 48 – 50 ]. Poor weight gain was mainly attributed to malabsorption [ 42 , 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of field strain of fowl aviadenovirus serotype 11 isolated from chickens with inclusion body hepatitis in Morocco

Abghour

Mouahid²,

Darkaoui³

et al. 2021

PLoS ONE

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Outbreaks of inclusion body hepatitis have emerged in Morocco since 2013 and has resulted in significant economic losses to poultry farms. Three isolates of the causative virus, Fowl adenonovirus (FAdV)were characterized from chickens with IBH, but their pathogenicity has never been investigated. In this work, the pathogenicity of an isolate FAdV 11 (MOR300315 strain) was evaluated by inoculating a group of 40 SPF chickens at 3 days of age by oral route. A group of 40 chicks injected with phosphate-buffered saline solution was used as a control group. The infected chickens showed decreased weight gain from 3dpi. Necropsy displayed pallor and enlargement in liver, swelling and slight hemorrhage in kidney and spleen at 6 dpi. Histopathological changes were mainly characterized by severe and extensive hepatic necrosis associated with the presence of basophilic intra-nuclear inclusion bodies within hepatocytes. The FAdV was reisolated in chicken embryo fibroblast cell culture from liver tissue homogenate of infected chicken from 3 to 6 dpi. Viral DNA was detected by PCR in liver, kidney, spleen and cloacal swabs from 3 to 13 dpi. Antibody response against inoculated FAdV was appeared from 9 dpi. These results confirmed that the FAdV 11 strain is pathogenic in chicken. This study is the first experimental infection of FAdV 11 in chicken in Morocco, which increase our understanding of its pathogenicity in chickens and indicate that preventive measures against FAdV infection in poultry farms should be implemented in Morocco.

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Section: Discussionmentioning

confidence: 99%

Pathogenicity of field strain of fowl aviadenovirus serotype 11 isolated from chickens with inclusion body hepatitis in Morocco

Abghour

Mouahid²,

Darkaoui³

et al. 2021

PLoS ONE

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“…TA B L E 3 Farm type and mortality rate on eight sampled farms and identification of viral nucleic acid for fowl adenovirus (FAdV), infectious bursal disease virus (IBDV) and chicken infectious anaemia virus (CIAV), and FAdV serotype, as determined by hexon loop-1 sequence analysis In a similar way to the majority of more recent FAdV screenings studies (Changjing et al, 2016;De la Torre et al, 2018;Zhang et al, 2016), we have applied molecular typing based on the hexon loop-1 for classification of the detected FAdV strains, replacing the traditional methods of serological cross-neutralization assays and restriction fragment length polymorphism (Hess, 2000). Although these tests have served as the basis for typing of isolates, these methods of characterization are costly, require reference sera and are sometimes unable to differentiate between serotypes (Changjing et al, 2016;Hess, 2000).…”

Section: Fadv-4 Has Been Implicated In Causing Hhs In Countries Of Southmentioning

confidence: 99%

Identification of four serotypes of fowl adenovirus in clinically affected commercial poultry co‐infected with chicken infectious anaemia virus in Trinidad and Tobago

Jordan

Blake

Bisnath³

et al. 2019

Transbound Emerg Dis

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Summary Fowl adenovirus (FAdV), which causes the high‐impact diseases such as inclusion body hepatitis and hepatitis‐hydropericardium syndrome, is of major concern to the poultry industry internationally. This study was carried out in direct response to mortality rates of up to 75% in commercial broiler flocks in Trinidad, West Indies. Symptoms in 3‐ to 8‐week‐old broilers and 13‐ to 18‐week‐old pullets pointed to infection with an immunosuppressive viral pathogen. The objectives of the study were to determine whether the infectious agent FAdV, along with other viral pathogens, was responsible for the clinical disease, and to obtain information on the serotypes of FAdV that were infecting the birds. Tissue samples from clinically affected birds from eight different farms were tested for chicken infectious anaemia virus (CIAV) and infectious bursal disease virus (IBDV) by real‐time reverse transcription polymerase chain reaction (PCR) and for FAdV by conventional PCR. The birds tested positive for FAdV and CIAV, but negative for IBDV. The gene corresponding to the L1 loop of the hexon protein for FAdV was amplified and sequenced. Phylogenetic analysis of seven FAdV strains inferred that four serotypes were likely to be circulating in the chickens. Well supported genetic relatedness was observed for serotype 8a (97.8%), 8b (97.8%), 9 (95.8%) and 11 (98.8%–99.5%). This is the first published report from Trinidad and Tobago on the presence and circulation of pathogenic FAdV strains, in combination with CIAV, in poultry. The data demonstrate a possible need for the introduction of serotype‐specific vaccines against FAdV, as well as vaccines against CIAV, in broilers in the region and emphasize the importance of maintaining high levels of biosecurity on farms to prevent the spread of these potentially devastating viruses between farms.

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“…In addition, several reports of IBH and/or HHS were documented in Japan ( 31 ), Korea ( 32 ), India ( 33 ), Pakistan ( 34 ), Spain ( 25 ), Poland ( 35 ), Iran ( 36 ), Mexico ( 37 ), Brazil ( 38 ), Croatia ( 39 ), Australia ( 40 ), Greece ( 41 ), Iraq ( 42 ), Palastine ( 43 ), United Arab Emirates ( 44 ), Saudi Arabia ( 45 ), Morocco ( 46 ), and South Africa ( 47 ).…”

Section: Current Status Of Ibh And/or Hhs Associated Problemsmentioning

confidence: 99%

Epidemiology, pathology, prevention, and control strategies of inclusion body hepatitis and hepatitis-hydropericardium syndrome in poultry: A comprehensive review

et al. 2022

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Infection with fowl adenoviruses (FAdVs) can result in a number of syndromes in the production of chicken, including inclusion body hepatitis (IBH), hepatitis-hydropericardium syndrome (HHS), and others, causing enormous economic losses around the globe. FAdVs are divided into 12 serotypes and five species (A–E; 1–8a and 8b−11). Most avian species are prone to infection due to the widespread distribution of FAdV strains. The genus aviadenovirus, which is a member of the adenoviridae family, is responsible for both IBH and HHS. The most popular types of transmission are mechanical, vertical, and horizontal. Hepatitis with basophilic intranuclear inclusion bodies distinguishes IBH, but the buildup of translucent or straw-colored fluid in the pericardial sac distinguishes HHS. IBH and HHS require a confirmatory diagnosis because their clinical symptoms and postmortem abnormalities are not unique to those conditions. Under a microscope, the presence of particular lesions and inclusion bodies may provide clues. Traditional virus isolation in avian tissue culture is more delicate than in avian embryonated eggs. Additionally, aviadenovirus may now be quickly and precisely detected using molecular diagnostic tools. Preventive techniques should rely on efficient biosecurity controls and immunize breeders prior to production in order to protect progeny. This current review gives a general overview of the current local and global scenario of IBH, and HHS brought on by FAdVs and covers both their issues and preventative vaccination methods.

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Molecular characterization of fowl adenovirus group I in commercial broiler chickens in Brazil

Cited by 19 publications

References 26 publications

Pathogenicity of field strain of fowl aviadenovirus serotype 11 isolated from chickens with inclusion body hepatitis in Morocco

Pathogenicity of field strain of fowl aviadenovirus serotype 11 isolated from chickens with inclusion body hepatitis in Morocco

Identification of four serotypes of fowl adenovirus in clinically affected commercial poultry co‐infected with chicken infectious anaemia virus in Trinidad and Tobago

Epidemiology, pathology, prevention, and control strategies of inclusion body hepatitis and hepatitis-hydropericardium syndrome in poultry: A comprehensive review

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