Baseline levels of metabolites in different tissues of mussel Mytilus galloprovincialis (Bivalvia: Mytilidae)

Cappello, Tiziana; Giannetto, Alessia; Parrino, Vincenzo; Maisano, Maria; Oliva, Sabrina; Marco, Giuseppe De; Guerriero, Giulia; Mauceri, Angela; Fasulo, Salvatore

doi:10.1016/j.cbd.2018.03.005

Cited by 42 publications

(41 citation statements)

References 59 publications

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“…Polar metabolites were extracted from gill tissues of pen shells by applying a "two-step" methanol/chloroform/water procedure, as described previously (Fasulo et al 2012;Cappello et al 2013Cappello et al , 2018b. In brief, gill tissues (c.a.…”

Section: Extraction Of Polar Metabolitesmentioning

confidence: 99%

Pen shell Pinna nobilis L. (Mollusca: Bivalvia) from different peculiar environments: adaptive mechanisms of osmoregulation and neurotransmission

Cappello

Giannetto

Natalotto

et al. 2019

The European Zoological Journal

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The pen shell Pinna nobilis (Linnaeus, 1758) is the largest endemic bivalve mollusc of the Mediterranean Sea, listed as an endangered species in the European Union. Because no information is available about the adaptation of pen shells to different habitats, herein the fundamental conditions of adaptation of P. nobilis to peculiar natural environments, such as the Strait of Messina (SM) and the meromictic Faro Lake (FL; Sicily, Italy), were explored by assessing the morphology, mucous production, osmoregulation and neurotransmission of their gills. Although gills of the pen shells from both sites exhibited a regular morphology, a higher presence of acid mucous cells was detected in P. nobilis from FL than SM, as well as higher levels of osmolytes but without interfering the osmoregulatory processes. About the functioning of gills, the cholinergic (i.e. acetylcholine and AChE) neuronal system was unaltered between individuals from the two sites, whereas the GABAergic neurotransmission (i.e. 4-aminobutyrate or GABA) was significantly augmented in gills of P. nobilis from FL than SM. This may be an adaptive response to hypoxic conditions in FL, as supported by the increased hypoxiainducible factor (HIF-1α) in gills of pen shells from FL than SM. Noteworthy, this study reports for the first time the presence of the GABA neurotransmitter within the metabolite profile, obtained by application of a protonic Nuclear Magnetic Resonance (1 H NMR)-based metabolomics approach, of a marine bivalve. Therefore, GABA may be suggested as a metabolite biomarker in pen shells. Overall, findings from this study provide new insights on the behavioural and adaptive responses of the pen shell Pinna nobilis settled in different peculiar environments.

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Section: Extraction Of Polar Metabolitesmentioning

confidence: 99%

Pen shell Pinna nobilis L. (Mollusca: Bivalvia) from different peculiar environments: adaptive mechanisms of osmoregulation and neurotransmission

Cappello

Giannetto

Natalotto

et al. 2019

The European Zoological Journal

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“…Marine mussels are exceptionally well adapted to live in coastal marine habitats, where they are exposed to fluctuating environmental conditions and elevated levels of natural and anthropogenic pollutants. For these reasons, they possess an antioxidant system that plays a key role in their remarkable capacity for acclimation [16][17][18]. In this study, the mussel (Mytilus galloprovincialis) was studied due to its relevant position as a pollution sentinel species in estuaries and coastal waters, where it is used in pollution monitoring programs.…”

Section: Introductionmentioning

confidence: 99%

“…Mussels are filter-feeders, which results in the accumulation of high levels of contaminants in their tissues, sometimes well over concentrations found in the water column [17]. In health assessment, mussels are used to measure biological endpoints that could provide information about the level of exposure to pollutants, or the effects of such exposures [17][18][19][20]. In this sense, there are different programs using oxidative stress responses to evaluate toxicological effects of different anthropic activities, accidental spills [17,20,21] or the effects of climate change [22].…”

Section: Introductionmentioning

confidence: 99%

Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters

et al. 2020

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Reactive oxygen species present a challenge for marine organisms releasing gametes into the water. Thiol-containing molecules protect cells against oxidative stress, and ovothiol (OSH), an antioxidant-reducing mercaptohistidine, has been described as especially relevant in the oocytes of marine invertebrates. Ovothiol synthase (ovoA), in charge of the first step in OSH synthesis, was sequenced in mussels, Mytilus galloprovincialis. Transcription levels of ovoA in mantle did not significantly change along the reproductive cycle. No alterations of ovoA transcription were observed after a laboratory copper (10 µg/L) exposure or in mussels captured in a highly polluted site. Conversely, the metabolomic analysis of the hydrophilic metabolite content in mantle clearly classified mussels according to their site of origin, especially at the most advanced stages of oogenesis. Quantification of OSH-A and -B and glutathione (GSH), revealed stable levels in mantle at early gametogenesis in the unpolluted sampling site, but a strong increase in female mantle previous to spawning in the polluted site. These increased concentrations under pollution suggest that OSH-A accumulates along oogenesis, independent of gene transcription regulation. The concerted accumulation of OSH-A and GSH suggests the building of a balanced cellular redox-system to scavenge ROS produced in the oocyte before and during fertilization.

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“…With multiple advantages of noninvasion, nondestruction, universal response, high throughput, and good resolution, NMR shows potent metabolic profiling capabilities and has been widely used for metabolomic analysis [10][11][12]. A recent study shows the role of metabolomics in order to assess the effects of environmental pollution and metabolomics research in the health and safety of marine organisms [13,14]. At present, there are related researches on aquaculture marine foods.…”

Section: Introductionmentioning

confidence: 99%

Discriminant Analysis of Traditional Chinese Medicinal Properties Based on Holistic Chemical Profiling by ¹H‐NMR Spectrometry

Zhang

Guo

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Medicinal property, which is closely related to drug chemical profiling, is the essence of traditional Chinese medicine (TCM) theory and has always been the focus of modern Chinese medicine. Based on dozens of classic and commonly used TCM herbs with recognized medicinal properties, the present study just aimed to investigate the feasibility and reliability of medicinal property discriminant by using 1H-NMR spectrometry, which provided a mass of spectral data showing holistic chemical profile for multivariate analysis and data mining, including principal component analysis (PCA), Fisher linear discriminant analysis (FLDA), and canonical discriminant analysis (CDA). By using FLDA for two-class recognition, a large majority of test herbs (59/61) were properly discriminated as cold or hot group, and the only two exceptions were Chuanbeimu (Fritillariae Cirrhosae Bulbus) and Rougui (Cinnamomi Cortex), suggesting that medicinal properties interrelate with flavor and body tropism, and all these factors together bring up medicinal property and efficacy. While by performing CDA, 98.4% of the original grouped herbs and 77.0% of the leave-one-out cross-validated grouped cases were correctly classified. The findings demonstrated that discriminant analysis based on holistic chemical profiling data by 1H-NMR spectrometry may provide a powerful alternative to have a deeper understanding of TCM medicinal property.

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Baseline levels of metabolites in different tissues of mussel Mytilus galloprovincialis (Bivalvia: Mytilidae)

Cited by 42 publications

References 59 publications

Pen shell Pinna nobilis L. (Mollusca: Bivalvia) from different peculiar environments: adaptive mechanisms of osmoregulation and neurotransmission

Pen shell Pinna nobilis L. (Mollusca: Bivalvia) from different peculiar environments: adaptive mechanisms of osmoregulation and neurotransmission

Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters

Discriminant Analysis of Traditional Chinese Medicinal Properties Based on Holistic Chemical Profiling by ¹H‐NMR Spectrometry

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Baseline levels of metabolites in different tissues of mussel Mytilus galloprovincialis (Bivalvia: Mytilidae)

Cited by 42 publications

References 59 publications

Pen shell Pinna nobilis L. (Mollusca: Bivalvia) from different peculiar environments: adaptive mechanisms of osmoregulation and neurotransmission

Pen shell Pinna nobilis L. (Mollusca: Bivalvia) from different peculiar environments: adaptive mechanisms of osmoregulation and neurotransmission

Gametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waters

Discriminant Analysis of Traditional Chinese Medicinal Properties Based on Holistic Chemical Profiling by 1H‐NMR Spectrometry

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Product

Resources

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Discriminant Analysis of Traditional Chinese Medicinal Properties Based on Holistic Chemical Profiling by ¹H‐NMR Spectrometry