C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development

Na, Huimin; Ponomarova, Olga; Giese, Gabrielle E.; Walhout, Albertha J.M.

doi:10.1016/j.celrep.2018.02.100

Cited by 23 publications

(17 citation statements)

References 32 publications

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“…Animals were washed in 0.9% saline until the solution was clear and then twice more (3–6 times total). Metabolites were extracted and analyzed as described previously ( Na et al, 2018 ). Briefly, 50 µL of a semi-soft pellet of animals was transferred to a 2 mL FastPrep tube (MP Biomedicals) and flash frozen with liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

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Caenorhabditis elegans methionine/S-adenosylmethionine cycle activity is sensed and adjusted by a nuclear hormone receptor

Giese

Walker

Ponomarova

et al. 2020

eLife

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Vitamin B12 is an essential micronutrient that functions in two metabolic pathways: the canonical propionate breakdown pathway and the methionine/S-adenosylmethionine (Met/SAM) cycle. In Caenorhabditis elegans, low vitamin B12, or genetic perturbation of the canonical propionate breakdown pathway results in propionate accumulation and the transcriptional activation of a propionate shunt pathway. This propionate-dependent mechanism requires nhr-10 and is referred to as ‘B12-mechanism-I’. Here, we report that vitamin B12 represses the expression of Met/SAM cycle genes by a propionate-independent mechanism we refer to as ‘B12-mechanism-II’. This mechanism is activated by perturbations in the Met/SAM cycle, genetically or due to low dietary vitamin B12. B12-mechanism-II requires nhr-114 to activate Met/SAM cycle gene expression, the vitamin B12 transporter, pmp-5, and adjust influx and efflux of the cycle by activating msra-1 and repressing cbs-1, respectively. Taken together, Met/SAM cycle activity is sensed and transcriptionally adjusted to be in a tight metabolic regime.

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Section: Methodsmentioning

confidence: 99%

“…After settling, supernatant was transferred to glass vials (Sigma) and dried by speed-vac overnight. MeOX-MSTFA derivatized samples were analyzed on an Agilent 7890B/5977B single quadrupole GC-MS equipped with an HP-5ms Ultra Inert capillary column (30 m × 0.25 mm×0.25 μm) using the same method as described ( Na et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Caenorhabditis elegans methionine/S-adenosylmethionine cycle activity is sensed and adjusted by a nuclear hormone receptor

Giese

Walker

Ponomarova

et al. 2020

eLife

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“…In addition to folate, all the other vitamins of the B complex were also reported to be essential for normal growth and development of C. briggsae in axenic medium: riboflavin, thiamine, pyridoxine, niacinamide, pantothenic acid, biotin and cobalamin [55, 184–186]. Among these, cobalamin (vitamin B12) was most extensively studied in the C. elegans model system [30, 187–189]. This vitamin has the unique property of being synthesised solely by archaea and bacteria [190].…”

Section: Vitaminsmentioning

confidence: 99%

The nutritional requirements of Caenorhabditis elegans

2019

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Animals require sufficient intake of a variety of nutrients to support their development, somatic maintenance and reproduction. An adequate diet provides cell building blocks, chemical energy to drive cellular processes and essential nutrients that cannot be synthesised by the animal, or at least not in the required amounts. Dietary requirements of nematodes, including Caenorhabditis elegans have been extensively studied with the major aim to develop a chemically defined axenic medium that would support their growth and reproduction. At the same time, these studies helped elucidating important aspects of nutrition-related biochemistry and metabolism as well as the establishment of C. elegans as a powerful model in studying evolutionarily conserved pathways, and the influence of the diet on health.

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“…C. elegans is a bacterivore, making it a highly suitable "simple" model organism to study vitamin B12 biology Zhang et al, 2017). For instance, we have previously found that bacterial diets high in vitamin B12 accelerate C. elegans development and change metabolic gene expression Na et al, 2018;Watson et al, 2014). One set of genes repressed by vitamin B12 comprise an alternative propionate breakdown pathway, or propionate shunt (Watson et al, 2016) (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

A Persistence Detector for Metabolic Network Rewiring in an Animal

Bulcha

Giese

Ali

et al. 2018

Preprint

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Here, we discover a transcriptional persistence detector in Caenorhabditis elegans involving the nuclear hormone receptors nhr-10 and nhr-68, which activates genes comprising a propionate shunt pathway. This shunt is used only when flux through the canonical, vitamin B12-dependent propionate breakdown pathway is perturbed. We propose that the propionate persistence detector functions to preferentially catabolize propionate through the canonical pathway to avoid spurious production of toxic shunt intermediates.

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C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development

Cited by 23 publications

References 32 publications

Caenorhabditis elegans methionine/S-adenosylmethionine cycle activity is sensed and adjusted by a nuclear hormone receptor

Caenorhabditis elegans methionine/S-adenosylmethionine cycle activity is sensed and adjusted by a nuclear hormone receptor

The nutritional requirements of Caenorhabditis elegans

A Persistence Detector for Metabolic Network Rewiring in an Animal

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