Steroid profiles in both blood serum and seminal plasma are not correlated and do not reflect sperm quality: Study on the male reproductive health of fifty young Swiss men

Zufferey, Fanny; Rahban, Rita; Garcia, A.; Gagnebin, Yoric; Bernard, Julien; Tonoli, David; Jeanneret, Fabienne; Stettler, Eric; Senn, A.; Nef, Serge; Rossier, Michel F.

doi:10.1016/j.clinbiochem.2018.03.008

Cited by 17 publications

(14 citation statements)

References 16 publications

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“…Ureaplasma is the smallest prokaryote between bacteria and viruses, mainly found in the genitourinary tract of the human body. Bacteroides ureolyticus is a species of Bacteroides that could impair sperm structure and function by diminishing motility and causing sperm membrane injury, especially to the lipid bilayers as shown in in vitro tests 29,40,41 . Another study using 16S rRNA sequencing demonstrated that genus Anaerococcus could be a biomarker for predicting male infertility 13 .…”

Section: Discussionmentioning

confidence: 99%

“…Compared to healthy controls, caspase-3 and cytochrome C levels were higher, and the total antioxidant capacity (TAC) was lower in seminal plasma of infertile patients 27,28 . Testosterone and androstenedione vectors correlate with steroids, which may function as biomarkers in patients with endocrine disorders, thus indicating that they may play an important role in sexual maturity 29 . A study also found that follicle-stimulating hormone deficiency could affect male fertility 30 .…”

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confidence: 99%

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Potential Pathogenic Bacteria in Seminal Microbiota of Patients with Different Types of Dysspermatism

Yang

Zhang

Xue

et al. 2020

Sci Rep

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Human microbiota play an important role in the health of their human hosts. Recent studies have demonstrated that microbiota exist in seminal plasma. The current study aims to elucidate whether seminal microbiota exist in patients with different types of dysspermatism and whether bacterial biomarkers can be identified for them. A total of 159 study participants were recruited, including 22 patients with oligoasthenospermia, 58 patients with asthenospermia, 8 patients with azoospermia, 13 patients with oligospermia, and 58 matched healthy controls. Seminal microbiota composition was analyzed using 16S rRNA gene-based sequencing. The results showed that the composition of seminal microbiota of patients with dysspermatism differed from those of healthy controls. Comparison of the microbiota composition in semen samples from patients with different types of dysspermatism showed that microbiota in patients with asthenospermia and oligoasthenospermia were distinct from healthy controls in beta diversity (P < 0.05). Characteristic biomarkers, including Ureaplasma, Bacteroides, Anaerococcus, Finegoldia, Lactobacillus and Acinetobacter lwoffii, were identified based on LEfSe analysis. Inferred functional analysis based on seminal microbiome data further indicated the presence of potential pathogenic biomarkers in patients with asthenospermia and oligoasthenospermia. These results provided profiles of seminal microbiota exhibited in different types of dysspermatism, thus providing new insights into their pathogenesis. Human microbiota, with its diverse relationships-commensal, parasitic, mutualistic, and pathogenic-play an important role in human health. Recent studies have reported that microbiota exist in almost every part of human body-even in the endocrine niche, such as in tumors, blood, and synovial fluid 1-4. Advances in technology and new research have demonstrated that microbiota are found in seminal plasma, and play an important role in host homeostasis 5. It has been demonstrated that the presence of bacteria in sperm is associated with male infertility 6. Some bacteria in the urogenital tract may affect spermatogenesis and decrease sperm quality through various means, including decrease in sperm motility, deficiency in DNA integrity, and destruction of mitochondrial function 7. Escherichia coli, Mycoplasma genitalium, Ureaplasma urealyticum, Mycoplasma hominis, Staphylococcus aureus, and Chlamydia trachomatis are pathogens associated with male infertility 8-12. Several studies using high-throughput sequencing have demonstrated that seminal plasma has a bacterial community, which includes Lactobacillus, Pseudomonas, Prevotella, and Gardnerella, among others 13-19 .

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Potential Pathogenic Bacteria in Seminal Microbiota of Patients with Different Types of Dysspermatism

Yang

Zhang

Xue

et al. 2020

Sci Rep

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“…Samples from 200 young men (age 18-22 years) were sequentially obtained from a large cohort of Swiss conscripts previously described by Rahban et al 26 and by Zufferey et al 25 Sperm samples were analysed in the context of the Swiss national study, 26 The total concentrations of BPA and BPS in urine were determined, and the presence of selected drugs (buprenorphine, paracetamol, oxycodone, fentanyl, tricyclic antidepressants, THC, cocaine, benzodiazepine, amphetamine and morphine/opiates) was screened with an immunological cassette as previously described. 25 Moreover, urinary THC-COOH (an inactive metabolite of THC), used as an indicator of cannabis consumption, was also measured by GC-MS according to a method previously reported. 25…”

Section: Subjects and Semen Analysesmentioning

confidence: 99%

“…25 Moreover, urinary THC-COOH (an inactive metabolite of THC), used as an indicator of cannabis consumption, was also measured by GC-MS according to a method previously reported. 25…”

Section: Subjects and Semen Analysesmentioning

confidence: 99%

“…21 Exposure to endocrine disruptors such as bisphenol A (BPA) or bisphenol S (BPS) [22][23][24] or the use of recreational drugs like cannabis 4 may be a cause of poor semen quality. We have recently reported 25 that steroid profiles, within a small cohort of fifty young Swiss men, are distinct in blood serum and in seminal fluid and that these compounds are poorly related to semen quality, as defined by sperm count, motility or velocity. However, the presence of specific drugs or xenobiotics, like THC, affected the seminal concentration of androgens, suggesting that the semen steroid profile could be used as a biological marker of exposure to putative endocrine disruptor compounds (EDC).…”

Section: Introductionmentioning

confidence: 99%

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Semen endocannabinoids are correlated to sperm quality in a cohort of 200 young Swiss men

et al. 2020

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Background A role for endocannabinoids in the male and female reproductive systems has been highlighted during the recent decades. Some of these compounds bind the cannabinoid CB1 receptor, which is abundantly expressed in the central nervous system but also present in the reproductive system, while others act as ‘entourage compounds’ modulators. Objectives The present study aimed at evaluating the relationship between sperm quality and endocannabinoid profiles in a cohort of 200 young Swiss men and whether the presence of specific xenobiotics could influence these profiles. Materials and methods Semen analysis was performed according to WHO guidelines. Endocannabinoid profiles in blood and semen, as well as bisphenol A and S in urine, were determined by LC‐MSMS methods. The presence of selected drugs was tested in urine by immunological screening, and urinary tetrahydrocannabinol (THC) metabolites were quantified by GC‐MS. Results Anandamide concentrations in seminal fluid and oleoylethanolamide (OEA) concentrations in blood serum appeared inversely correlated with sperm motility, while semen palmytoylethanolamide (PEA) was positively linked to sperm concentration. Moreover, OEA and PEA in seminal fluid were associated with better sperm morphology. Interestingly, the concentrations of the same endocannabinoids measured in both blood and semen were not correlated, and the presence of THC metabolites in some individuals was linked to lower concentrations of endocannabinoids. Conclusions In the context of the general decline of the sperm count observed within the male population, endocannabinoids in semen constitute a class of promising biochemical markers that open new perspectives as a complement for the usual evaluation of semen quality or for the toxicological screening of individuals' exposure to putative endocrine disruptors.

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Sexually transmitted doping: The impact of urine contamination of semen

Handelsman

Bacha

DeBono

et al. 2022

Drug Testing and Analysis

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The high sensitivity of antidoping detection tests creates the possibility of inadvertent doping due to an athlete's unknowing ingestion of contaminated environmental sources such as dietary supplements, food, or drinks. Recently, athletes denying use of a prohibited substance have claimed that the positive antidoping tests was due to exchange of bodily fluids with a nonathlete partner using a prohibited substance.Measurement of drugs in semen is largely limited to one or very few samples due to the inaccessibility of sufficiently frequent semen samples for detailed pharmacokinetics. An emerging issue in semen drug measurements is that semen samples may contain residual urine from ejaculation left in the urethra; however, the urine content in semen samples has not been studied. In the present study, we employed concurrent creatinine measurements in urine and seminal plasma to determine the urine content of semen samples.

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Steroid profiles in both blood serum and seminal plasma are not correlated and do not reflect sperm quality: Study on the male reproductive health of fifty young Swiss men

Cited by 17 publications

References 16 publications

Potential Pathogenic Bacteria in Seminal Microbiota of Patients with Different Types of Dysspermatism

Potential Pathogenic Bacteria in Seminal Microbiota of Patients with Different Types of Dysspermatism

Semen endocannabinoids are correlated to sperm quality in a cohort of 200 young Swiss men

Sexually transmitted doping: The impact of urine contamination of semen

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