Influence of diameter of fiber membrane scaffolds on the biocompatibility of hPDL mesenchymal stromal cells

Suárez-Franco, J.L.; Vázquez-Vázquez, Febe Carolina; Pozos‐Guillén, Amaury; Montesinos, Juan José; Álvarez-Fregoso, O.; Álvarez-Pérez, Marco Antonio

doi:10.4012/dmj.2016-329

Cited by 16 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scaffolds were prepare as previously described (16). Briefly, 7% wt of PLA pellets (C3H6O3; MW 192,000; Nature Works, MN, USA) were dissolved in chloroform (CHCl3), and stirred for 20 h. Subsequently anhydrous absolute ethyl alcohol (CH3CH2OH, J.T.…”

Section: Preparation Of Pla Scaffolds By Air Jet Spinningmentioning

confidence: 99%

Physicochemical and Tissue Response of PLA Nanofiber Scaffolds Sterilized by Different Techniques

Mendieta-Barrañon

Chanes-Cuevas

Álvarez-Pérez

et al. 2018

Odovtos - Int J Dent Sc

View full text Add to dashboard Cite

In recent years, tissue engineering has evolved considerably, due to the problems in the biomedical area concerning tissue regeneration therapies. Currently, work has been focused on the synthesis and physicochemical characterization of poly lactic acid scaffolds, a synthetic polyester that has been extensively study for its excellent biocompatibility and biodegradability. Moreover, sterilization strategies of scaffold are a crucial step for its application in tissue regeneration, however, the sterilization process have to maintain the structural and biochemical properties of the scaffold. Therefore, it is very important to carry out studies on the sterilization methods of the sample’s material, since translational medicine is intended for in vivo applications. The aim of the present study was designed to analyze the effects of different sterilization techniques, i.e. ethylene oxide (ETO), gamma radiation (GR) and hydrogen peroxide-based plasma (H2O2) in biodegradable PLA scaffolds, and to determine the best sterilization technique to render a sterile product with minimal degradation and deformation, and good tissue response. Analysis of surface morphology showed that ETO and GR modified the PLA scaffolds without any change in its chemical composition. Moreover, the histological response showed that the scaffolds are biocompatible and those sterilized by GR showed a more severe inflammatory response, accompanied with the presence of giant foreign body cells. In conclusion, the results show that among sterilization techniques used in the preset study, the best results were observed with H2O2 sterilization, since it did not significantly modify the surface structure of the PLA fibers and their in vivo response did not cause an unfavorable tissue reaction.

show abstract

Section: Preparation Of Pla Scaffolds By Air Jet Spinningmentioning

confidence: 99%

Physicochemical and Tissue Response of PLA Nanofiber Scaffolds Sterilized by Different Techniques

Mendieta-Barrañon

Chanes-Cuevas

Álvarez-Pérez

et al. 2018

Odovtos - Int J Dent Sc

View full text Add to dashboard Cite

show abstract

“…Next, Figure 6b shows FTIR measurements of PAN nanofiber mats on 3D printed PLA. Figure 6a gives an overview of typical peaks of PLA [51,52,53,54] (grey lines) and PAN [55] (red lines), respectively.…”

Section: Resultsmentioning

confidence: 99%

Stabilization of Electrospun Nanofiber Mats Used for Filters by 3D Printing

et al. 2019

View full text Add to dashboard Cite

Electrospinning is a well-known technology used to create nanofiber mats from diverse polymers and other materials. Due to their large surface-to-volume ratio, such nanofiber mats are often applied as air or water filters. Especially the latter, however, have to be mechanically highly stable, which is challenging for common nanofiber mats. One of the approaches to overcome this problem is gluing them on top of more rigid objects, integrating them in composites, or reinforcing them using other technologies to avoid damage due to the water pressure. Here, we suggest another solution. While direct 3D printing with the fused deposition modeling (FDM) technique on macroscopic textile fabrics has been under examination by several research groups for years, here we report on direct FDM printing on nanofiber mats for the first time. We show that by choosing the proper height of the printing nozzle above the nanofiber mat, printing is possible for raw polyacrylonitrile (PAN) nanofiber mats, as well as for stabilized and even more brittle carbonized material. Under these conditions, the adhesion between both parts of the composite is high enough to prevent the nanofiber mat from being peeled off the 3D printed polymer. Abrasion tests emphasize the significantly increased mechanical properties, while contact angle examinations reveal a hydrophilicity between the original values of the electrospun and the 3D printed materials.

show abstract

“…The spectra of nanofibers show the general PLA bands, including the CH 3 asymmetric stretching at 2948.6 cm −1 , C=O stretching at 1748.2 cm −1 and C-0=0 stretching at 1181.2 cm −1 and 1038.8 cm −1 , which is a characteristic ester bond. Bending vibrations for CH 3 asymmetric and CH 3 symmetric were identified at 1453.1 cm −1 and 1381.7 cm −1 , respectively [32,33]. PVA shows the typical infrared bands of hydrocyl groups at 3200–3600 cm −1 which can not be seen in the core-shell structured nanofibers.…”

Section: Resultsmentioning

confidence: 99%

Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein

Sumeyra

Ulkugul

et al. 2019

Medicina

View full text Add to dashboard Cite

Background and Objectives: A coaxial electrospinning technique was used to produce core/shell nanofibers of a polylactic acid (PLA) as a shell and a polyvinyl alcohol (PVA) containing metformin hydrochloride (MH) as a core. Materials and Methods: Fish sarcoplasmic protein (FSP) was extracted from fresh bonito and incorporated into nanofiber at various concentrations to investigate the influence on properties of the coaxial nanofibers. The morphology, chemical structure and thermal properties of the nanofibers were studied. Results: The results show that uniform and bead-free structured nanofibers with diameters ranging from 621 nm to 681 nm were obtained. A differential scanning calorimetry (DSC) analysis shows that FSP had a reducing effect on the crystallinity of the nanofibers. Furthermore, the drug release profile of electrospun fibers was analyzed using the spectrophotometric method. Conclusions: The nanofibers showed prolonged and sustained release and the first order kinetic seems to be more suitable to describe the release. MTT assay suggests that the produced drug and protein loaded coaxial nanofibers are non-toxic and enhance cell attachment. Thus, these results demonstrate that the produced nanofibers had the potential to be used for diabetic wound healing applications.

show abstract

Influence of diameter of fiber membrane scaffolds on the biocompatibility of hPDL mesenchymal stromal cells

Cited by 16 publications

References 36 publications

Physicochemical and Tissue Response of PLA Nanofiber Scaffolds Sterilized by Different Techniques

Physicochemical and Tissue Response of PLA Nanofiber Scaffolds Sterilized by Different Techniques

Stabilization of Electrospun Nanofiber Mats Used for Filters by 3D Printing

Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein

Contact Info

Product

Resources

About