Investigation of the effects of thymoquinone on erythrocyte deformability in sepsis treatment which created by cecal perforation in rat

Bostancı, Hasan; Dikmen, Kürşat; Çomu, Faruk Metin; Arslan, M; Küçük, Ayşegül

doi:10.4149/bll_2018_029

Cited by 4 publications

(4 citation statements)

References 5 publications

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“…Leptocytes and codocytes were noted in hepatic insufficiency, especially in dogs and cats with portocaval shunts [ 27 , 29 ]. Sepsis leads to decreased red blood cell deformability in rats and mice [ 30 , 31 ] and altered erythrocyte morphology, with the formation of echinocytes, sphero-echinocytes, and spherostomatocytes [ 32 ]. These changes are thought to either be a combined result of oxygen free radicals and toxin action, or depletion of ATP in cells [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Changes in Leukogram and Erythrogram Results in Bitches with Vaginitis

Chmurska-Gąsowska

Bojarski

Sowińska

et al. 2021

Animals

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Vaginitis in female dogs is a problem most veterinarians face in their practice. It manifests as localized inflammation, and its variable etiology and different severities often make diagnosis problematic. The study consisted of comparing blood smears taken from 16 animals: 8 healthy bitches and 8 bitches with confirmed vaginitis. We analyzed the percentage of different types of white blood cells (leukogram) and changes in the shape of red blood cells (erythrogram) in both groups. We observed changes in red blood cell morphology, i.e., a higher percentage of lacrimocytes and schistocytes in female dogs with vaginitis compared to their healthy counterparts. The observed hematological changes may illustrate the severity of inflammation. The analysis of erythrograms showed a significantly higher percentage of lacrimocytes and schistocytes in diseased bitches (1.58 ± 1.19% and 0.13 ± 0.12%) compared to healthy animals (0.58 ± 0.38 and 0.00 ± 0.00, respectively). The obtained results may indicate that the analysis of erythrograms throughout the course of vaginitis in bitches may constitute a diagnostic tool, as opposed to the analysis of leukograms, which is more sensitive when it comes to the systemic inflammatory response of the organism. It seems that simultaneous analysis of erythrograms and leukograms may facilitate the diagnostic process in clinical practice.

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Section: Discussionmentioning

confidence: 99%

Changes in Leukogram and Erythrogram Results in Bitches with Vaginitis

Chmurska-Gąsowska

Bojarski

Sowińska

et al. 2021

Animals

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“…In order to create a sepsis model, cecal ligation and puncture (CLP) procedure were performed on 44 rats as previously described (16,17). 8 rats were excluded from the study because they died.…”

Section: Methodsmentioning

confidence: 99%

Effect of fluid resuscitation on acute lung injury in a rat model of sepsis

Erdoğan¹,

Erdoğan²,

Kara³

et al. 2021

BLL

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AIM: Sepsis is a systemic infection reaction and intravascular volume therapy plays a crucial role in it's treatment. Acute respiratory distress syndrome (ARDS) occurs in the lungs, the most affected organ. This study aimed to investigate the different effects of fl uid therapy on ARDS caused by sepsis. METHOD: To form a sepsis model, cecal ligation and puncture (CLP) procedure were performed on 44 adult rats. Divided into six groups; normal, CLP group, those treated with 40 ml/kg 0.9 % NaCl, 3 % NaCl (hypertonic saline), Ringer Lactate and Hydroxyethyl starch. After 24 hours treatments, histopathological examination of the lungs were done, and the plasma levels of CRP, TNF-α and IL-6 and paO 2 were measured. RESULTS: The scores of all histological parameters of the group treated with hypertonic saline were signifi cantly lower than of the other groups (p < 0.001). Likewise, according to the arterial blood gas results, paO 2 was signifi cantly higher (p < 0.01) in the hypertonic saline group compared to the other groups, and paCO 2 was signifi cantly lower (p < 0.01). CRP, TNF-α and IL-6 levels of infl ammatory markers were also signifi cantly lower in hypertonic saline groups compared to other groups (p < 0.001). CONCLUSIONS: Our study shows that treatment with hypertonic saline reduces the progression of ARDS in sepsis (Tab.

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“…Moreover, thymoquinone was found to prevent sequels of the multiple organ failure syndrome of sepsis via regulating the production of NO and its inflammatory sequela, and adjustment of vascular responsiveness (Alkharfy et al, 2015). Thymoquinone restored the erythrocyte deformability observed in CLP-induced sepsis in rats, showing more effectiveness when applied before the establishment of the sepsis (Bostanci et al, 2018). Ozer et al (2017) found that thymoquinone intraperitoneally injected in rats at 1 mg/kg exhibited protective effects on mesenteric perfusion, contractile function of aorta as well as anti-inflammatory and antioxidative effects.…”

Section: Thymoquinonementioning

confidence: 97%

Plants’ bioactive secondary metabolites in the management of sepsis: Recent findings on their mechanism of action

et al. 2022

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Sepsis is a severe inflammatory response to systemic infection and is a threatening cause of death in intensive care units. In recent years, a number of studies have been conducted on the protective effect of natural products against sepsis-induced organ injury. However, a comprehensive review of these studies indicating the mechanisms of action of the bioactive compounds is still lacking. In this context, this review aimed to provide an updated analysis of the mechanism of action of plants’ secondary metabolites in the management of sepsis. Scopus, Science Direct, Google Scholar, and PubMed were searched from inception to July 2022. A variety of secondary metabolites were found to be effective in sepsis management including allicin, aloin, cepharanthine, chrysin, curcumin, cyanidin, gallic acid, gingerol, ginsenoside, glycyrrhizin, hesperidin, kaempferol, narciclasine, naringenin, naringin, piperine, quercetin, resveratrol, rosmarinic acid, shogaol, silymarin, sulforaphane, thymoquinone, umbelliferone, and zingerone. The protective effects exerted by these compounds can be ascribed to their antioxidant properties as well as induction of endogenous antioxidant mechanisms, and also via the downregulation of inflammatory response and reduction of biochemical and inflammatory markers of sepsis. These findings suggest that these secondary metabolites could be of potential therapeutic value in the management of sepsis, but human studies must be performed to provide strength to their potential clinical relevance in sepsis-related morbidity and mortality reduction.

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Investigation of the effects of thymoquinone on erythrocyte deformability in sepsis treatment which created by cecal perforation in rat

Cited by 4 publications

References 5 publications

Changes in Leukogram and Erythrogram Results in Bitches with Vaginitis

Changes in Leukogram and Erythrogram Results in Bitches with Vaginitis

Effect of fluid resuscitation on acute lung injury in a rat model of sepsis

Plants’ bioactive secondary metabolites in the management of sepsis: Recent findings on their mechanism of action

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