Detection of qnrVC6, within a new genetic context, in an NDM-1-producing Citrobacter freundii clinical isolate from Uruguay

Bado, Inés; Papa-Ezdra, Romina; Cordeiro, Nicolás F.; Outeda, Matilde; Caiata, Leticia; García-Fulgueiras, Virginia; Seija, Verónica; Vignoli, Rafael

doi:10.1016/j.jgar.2018.02.023

Cited by 11 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the qnrVC6 gene, usually found in V. cholerae, was here detected in V. anguillarum 28AD. This is not really surprising, since qnrVC-like genes are currently spreading in other Vibrio species and Gram negatives such as Pseudomonas aeruginosa, Pseudomonas putida and Citrobacter freundii [71,72]. Interestingly, although the Qnr-like genes should provide resistance to quinolones, the phenotypes of all our strains are susceptible to ciprofloxacin and nalidixic acid, the two fluoroquinolones tested for.…”

Section: Discussionmentioning

confidence: 76%

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

et al. 2020

View full text Add to dashboard Cite

Antimicrobial resistance is a major public health concern restricted not only to healthcare settings but also to veterinary and environmental ones. In this study, we analyzed, by whole genome sequencing (WGS) the resistome, mobilome and virulome of 12 multidrug-resistant (MDR) marine strains belonging to Shewanellaceae and Vibrionaceae families collected at aquaculture centers in Italy. The results evidenced the presence of several resistance mechanisms including enzyme and efflux pump systems conferring resistance to beta-lactams, quinolones, tetracyclines, macrolides, polymyxins, chloramphenicol, fosfomycin, erythromycin, detergents and heavy metals. Mobilome analysis did not find circular elements but class I integrons, integrative and conjugative element (ICE) associated modules, prophages and different insertion sequence (IS) family transposases. These mobile genetic elements (MGEs) are usually present in other aquatic bacteria but also in Enterobacteriaceae suggesting their transferability among autochthonous and allochthonous bacteria of the resilient microbiota. Regarding the presence of virulence factors, hemolytic activity was detected both in the Shewanella algae and in Vibrio spp. strains. To conclude, these data indicate the role as a reservoir of resistance and virulence genes in the environment of the aquatic microbiota present in the examined Italian fish farms that potentially might be transferred to bacteria of medical interest.

show abstract

Section: Discussionmentioning

confidence: 76%

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Citrobacter spp., Klebsiella spp., and Salmonella spp.) and Pseudomonadaceae (P. aeruginosa and P. putida), among others (206,231,249,302,350,(364)(365)(366) (Fig. 5).…”

Section: Figmentioning

confidence: 98%

“…Similar to QnrB, another possible initial codon may be present 13 amino acids before the initial ATG codon (e.g., GenBank accession number APA29731). These alleles have been described worldwide in plasmid and integron environments, either in the first variable region of a classical class 1 integron or downstream from ISCR1 within a complex class 1 integron (274,364). In this sense, it is worth mentioning that qnrVC may be included in a gene cassette with an attC site (175,231,361).…”

Section: Figmentioning

confidence: 99%

Transferable Mechanisms of Quinolone Resistance from 1998 Onward

Ruiz

2019

Clin Microbiol Rev

115

View full text Add to dashboard Cite

SUMMARY While the description of resistance to quinolones is almost as old as these antimicrobial agents themselves, transferable mechanisms of quinolone resistance (TMQR) remained absent from the scenario for more than 36 years, appearing first as sporadic events and afterward as epidemics. In 1998, the first TMQR was soundly described, that is, QnrA. The presence of QnrA was almost anecdotal for years, but in the middle of the first decade of the 21st century, there was an explosion of TMQR descriptions, which definitively changed the epidemiology of quinolone resistance. Currently, 3 different clinically relevant mechanisms of quinolone resistance are encoded within mobile elements: (i) target protection, which is mediated by 7 different families of Qnr (QnrA, QnrB, QnrC, QnrD, QnrE, QnrS, and QnrVC), which overall account for more than 100 recognized alleles; (ii) antibiotic efflux, which is mediated by 2 main transferable efflux pumps (QepA and OqxAB), which together account for more than 30 alleles, and a series of other efflux pumps (e.g., QacBIII), which at present have been sporadically described; and (iii) antibiotic modification, which is mediated by the enzymes AAC(6′)Ib-cr, from which different alleles have been claimed, as well as CrpP, a newly described phosphorylase.

show abstract

“…This indicates that important antibiotic-resistant pathogens are being somehow 223 transmitted among clinical settings and the urban environment, representing a public health threat. However, 224 other ARGs such as metallo-β-lactamases that have also been reported in Uruguay [31][32][33][34] were not found in 225 the sewage or beaches. Beyond technical biases are possible, this can be attributed to a differential capacity 226 between distinct antibiotic-resistant clones to survive and spread in the environment; given that selective 227 fitness of antibiotic-resistant pathogens (adapted to high antibiotic pressures in hospital settings) may be 228 lower in less-exposed environmental waters [35].…”

mentioning

confidence: 99%

City-wide metagenomics uncover antibiotic resistance reservoirs in urban beach and sewage waters

Fresia

Antelo

Salazar

et al. 2018

Preprint

View full text Add to dashboard Cite

41Background: Microbial communities present in environmental waters constitute a reservoir for antibiotic-42 resistant pathogens that impact human health. For this reason a diverse variety of water environments are 43 being analyzed using metagenomics to uncover public health threats. However, the composition of these 44 communities along the coastal environment of a whole city where sewage and beach waters are mixed, is 45 poorly understood. 47Results: We shotgun-sequenced 20 coastal areas from the city of Montevideo (capital of Uruguay) including 48 beach and sewage water samples to characterize bacterial communities and their virulence and antibiotic 49 resistance repertories. We found that sewage and beach environments presented significantly different 50 bacterial communities. Sewage waters harbored a higher prevalence and a more diverse repertory of virulence 51 and antibiotic resistant genes mainly from well-known enterobacteria, including carbapenemases and 52 extended-spectrum betalactamases reported in hospital infections in Montevideo. Additionally, we were able 53 to genotype the presence of both globally-disseminated pathogenic clones as well as emerging antibiotic-54 resistant bacteria in sewage waters. 56Conclusions: Our study represents the first in using metagenomics to jointly analyze beaches and the sewage 57 system from an entire city, allowing us to characterize antibiotic-resistant pathogens circulating in urban 58 waters. The data generated in this initial study represent a baseline metagenomic exploration to guide future 59 longitudinal (time-wise) studies, whose systematic implementation will provide useful epidemiological 60 information to improve public health surveillance. 61 62

show abstract

Detection of qnrVC6, within a new genetic context, in an NDM-1-producing Citrobacter freundii clinical isolate from Uruguay

Cited by 11 publications

References 18 publications

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

Transferable Mechanisms of Quinolone Resistance from 1998 Onward

City-wide metagenomics uncover antibiotic resistance reservoirs in urban beach and sewage waters

Contact Info

Product

Resources

About