Leucaena leucocephala pod seed protein as an alternate to animal protein in fish feed and evaluation of its role to fight against infection caused by Vibrio harveyi and Pseudomonas aeruginosa

Verma, Vipin; Rani, Kumari Vandana; Kumar, Shiva Raj; Prakash, Om

doi:10.1016/j.fsi.2018.03.011

Cited by 16 publications

(7 citation statements)

References 53 publications

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“…Usually, bactericidal effects of leukocytes and phagocytosis were exhibited positive correlations with immune levels of host fish [ 48 ]. Zheng et al [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Oral vaccination with recombinant Lactobacillus casei expressing Aeromonas hydrophila Aha1 against A. hydrophila infections in common carps

et al. 2022

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The immunogenicity of Aha1, an OMP of Aeromonas hydrophila mediating the adhesion of bacteria onto the mucosal surface of hosts has been established. In this study, recombinant vectors, pPG1 and pPG2, carrying a 1366 bp DNA fragment that was responsible for encoding the 49 kDa Aha1 from A. hydrophila were constructed, respectively, then electroporated into a probiotic strain Lactobacillus casei CC16 separately to generate two types of recombinants, L. casei- pPG1-Aha1 (Lc-pPG1-Aha1) and L. casei -pPG2-Aha1 (Lc-pPG2-Aha1). Subsequently, these were orally administered into common carps to examine their immunogenicity. The expression and localization of the expressed Aha1 protein relative to the carrier L. casei was validated via Western blotting, flow cytometry, and immune fluorescence separately. The recombinant vaccines produced were shown high efficacies, stimulated higher level of antibodies and AKP, ACP, SOD, LZM, C3, C4 in serum in hosts. Immune-related gene expressions of cytokines including IL-10, IL-1β, TNF-α, IFN-γ in the livers, spleens, HK, and intestines were up-regulated significantly. Besides, a more potent phagocytosis response was observed in immunized fish, and higher survival rates were presented in common carps immunized with Lc-pPG1-Aha1 (60%) and Lc-pPG2-Aha1 (50%) after re-infection with virulent strain A. hydrophila . Moreover, the recombinant L. casei were shown a stronger propensity for survivability in the intestine in immunized fish. Taken together, the recombinant L. casei strains might be promising candidates for oral vaccination against A. hydrophila infections in common carps.

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“…Usually, bactericidal effects of leukocytes and phagocytosis were exhibited positive correlations with immune levels of host fish [ 48 ]. Zheng et al [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Oral vaccination with recombinant Lactobacillus casei expressing Aeromonas hydrophila Aha1 against A. hydrophila infections in common carps

et al. 2022

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“…Some have been commercialized particularly in the Far East for use with fish and shellfish (e.g., Geng et al 2012). Other examples for use in fish include β-1,3-glucan (Lee et al 2018), bovine lactoferrin (Esmaeili et al 2019), Chaga mushroom (Harikrishnan et al 2012a), the seed of the evergreen tree Leucaena leucocephala (Verma et al 2018), garlic (Allium sativum) (Talpur and Ikwanuddin 2012), ginger (Zingiber officinale), green tea (Camellia sinensis) (Harikrishnan et al 2011b), hawthorn (Crataegus sp.) extract (Tan et al 2017), Japanese pepper tree (Zanthoxylum piperitum) (Harikrishnan et al 2012d), kudzu (Pueraria thunbergiana) (Harikrishnan et al 2012b), loquat tree (Eriobotrya japonica) (Kim et al 2011), and pericarp (Zanthoxylum piperitum) (Harikrishnan et al 2012a).…”

Section: Dietary Supplementsmentioning

confidence: 99%

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Zhang

Austin

2020

Mar Life Sci Technol

183

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Vibrio harveyi, which belongs to family Vibrionaceae of class Gammaproteobacteria, includes the species V. carchariae and V. trachuri as its junior synonyms. The organism is a well-recognized and serious bacterial pathogen of marine fish and invertebrates, including penaeid shrimp, in aquaculture. Diseased fish may exhibit a range of lesions, including eye lesions/blindness, gastro-enteritis, muscle necrosis, skin ulcers, and tail rot disease. In shrimp, V. harveyi is regarded as the etiological agent of luminous vibriosis in which affected animals glow in the dark. There is a second condition of shrimp known as Bolitas negricans where the digestive tract is filled with spheres of sloughed-off tissue. It is recognized that the pathogenicity mechanisms of V. harveyi may be different in fish and penaeid shrimp. In shrimp, the pathogenicity mechanisms involved the endotoxin lipopolysaccharide, and extracellular proteases, and interaction with bacteriophages. In fish, the pathogenicity mechanisms involved extracellular hemolysin (encoded by duplicate hemolysin genes), which was identified as a phospholipase B and could inactivate fish cells by apoptosis, via the caspase activation pathway. V. harveyi may enter the so-called viable but nonculturable (VBNC) state, and resuscitation of the VBNC cells may be an important reason for vibriosis outbreaks in aquaculture. Disease control measures center on dietary supplements (including probiotics), nonspecific immunostimulants, and vaccines and to a lesser extent antibiotics and other antimicrobial compounds.

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“…NO, LSZ and the complement system play an important role in non-specific and specific immunity in fish (Güllü et al, 2016;Kumar et al, 2016;Meng et al, 2019;Rodriguez-Ramos et al, 2016;Sun et al, 2018;Verma, Rani, Kumar, & Prakash, 2018;Yuan et al, 2017).…”

Section: 433mentioning

confidence: 99%

Effects of ferulic acid on growth performance, immunity and antioxidant status in genetically improved farmed tilapia ( Oreochromis niloticus ) fed oxidized fish oil

Huang

Jiang

et al. 2020

Aquacult. Nutr.

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A study was conducted to characterize the effects of dietary oxidized fish oil on the growth performance, immunity and antioxidant status of genetically improved farmed tilapia (Oreochromis niloticus) and to determine the role of ferulic acid on the oxidative damage induced by the oxidized fish oil. The tilapia (13.73 ± 0.31 g) were fed four experimental diets containing untreated (peroxide value, POV: 2.2 meq/kg) and highly oxidized (POV: 120.6 meq/kg) fish oil either with or without ferulic acid (0 or 400 mg/kg) supplementation for 12 weeks. From the results, the oxidized fish oil treatments increased antioxidant enzyme activities and MDA values but decreased the weight gain and the immunological parameters in tilapia. Meanwhile, the serum biochemical indices were significantly affected by the oxidized fish oil. Besides, the addition of ferulic acid partially counteracted the free radical‐induced damage and improved the health status of tilapia. In conclusion, the oxidized fish oil may induce oxidative stress, destroy liver, dysregulate lipid metabolism as well as reduce non‐specific immunity, and eventually result in growth inhibition of tilapia. The ferulic acid supplementation partially offset the negative effects of the oxidized fish oil on tilapia.

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Leucaena leucocephala pod seed protein as an alternate to animal protein in fish feed and evaluation of its role to fight against infection caused by Vibrio harveyi and Pseudomonas aeruginosa

Cited by 16 publications

References 53 publications

Oral vaccination with recombinant Lactobacillus casei expressing Aeromonas hydrophila Aha1 against A. hydrophila infections in common carps

Oral vaccination with recombinant Lactobacillus casei expressing Aeromonas hydrophila Aha1 against A. hydrophila infections in common carps

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Effects of ferulic acid on growth performance, immunity and antioxidant status in genetically improved farmed tilapia ( Oreochromis niloticus ) fed oxidized fish oil

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