Second‐harmonic imaging microscopy for identifying colorectal intraepithelial neoplasia

Li, L.H.; Jiang, Weizhong; Kang, Deyong; Liu, X.; Li, H.S.; Guan, Guoxian; Zhuo, Shuangmu; Chen, Z.F.; Chen, J.X.

doi:10.1111/jmi.12690

Cited by 3 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Already at one month after both treatments, changes in the colon collagen structure were visible. Alteration of the collagen scaffold was already observed in other CRC-based studies using SHG microscopy for detection of changes in the structure of tissues [ 66 , 67 , 68 ]. Variation of symmetry as well as of collagen accumulation density in both conditions strongly highlight similar pathogenic mechanisms, i.e., response to inflammation and IL-6 activity.…”

Section: Discussionmentioning

confidence: 78%

Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa

Čaja

Stakheev

Chernyavskiy

et al. 2021

Cancers

View full text Add to dashboard Cite

Continuous activation of the immune system inside a tissue can lead to remodelling of the tissue structure and creation of a specific microenvironment, such as during the tumour development. Chronic inflammation is a central player in stimulating changes that alter the tissue stroma and can lead to fibrotic evolution. In the colon mucosa, regulatory mechanisms, including TGF-β1, avoid damaging inflammation in front of the continuous challenge by the intestinal microbiome. Inducing either DSS colitis or AOM colorectal carcinogenesis in AVN-Wistar rats, we evaluated at one month after the end of each treatment whether immunological changes and remodelling of the collagen scaffold were already in development. At this time point, we found in both models a general downregulation of pro-inflammatory cytokines and even of TGF-β1, but not of IL-6. Moreover, we demonstrated by multi-photon microscopy the simultaneously presence of pro-fibrotic remodelling of the collagen scaffold, with measurable changes in comparison to the control mucosa. The scaffold was significantly modified depending on the type of induced stimulation. These results suggest that at one month after the end of the DSS or AOM inductions, a smouldering inflammation is present in both induced conditions, since the pro-inflammatory cytokines still exceed, in proportion, the local homeostatic regulation of which TGF-β1 is a part (inflammatory threshold). Such an inflammation appears sufficient to sustain remodelling of the collagen scaffold that may be taken as a possible pathological marker for revealing pre-neoplastic inflammation.

show abstract

Section: Discussionmentioning

confidence: 78%

Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa

Čaja

Stakheev

Chernyavskiy

et al. 2021

Cancers

View full text Add to dashboard Cite

show abstract

Polarization Microscopy in Biomedical Applications

Spandana

Melanthota

Raju

et al. 2023

Optical Polarimetric Modalities for Biomedical Research

View full text Add to dashboard Cite

Label-free quantification of imaging features in the extracellular matrix of left and right-sided colon cancer tissues

Arora,

Kulkarni,

Markus

et al. 2024

Sci Rep

View full text Add to dashboard Cite

The molecular pathogenesis of colorectal cancer is known to differ between the right and left side of the colon. Several previous studies have focussed on the differences in clinicopathological features, proteomic and genetic biomarkers, the composition of gut microbiota, response to therapy, and the characteristics of the tumour microenvironment. However, the morphology and density of collagen in the extracellular matrix (ECM) have not been studied intensively. In this study, we employed 2-photon laser scanning microscopy (2PLSM) to visualise the intrinsic second-harmonic generation (SHG) signal emitted by collagen fibres in the heterogeneous ECM of human colon tumour tissues. Through texture analysis of the SHG signal, we quantitatively distinguished the imaging features generated by structural differences of collagen fibres in healthy colon and cancers and found marked differences. The fibres inside of tumours exhibited a loss of organisation, particularly pronounced in right-sided colon cancer (RSCC), where the chaotic regions were significantly increased. In addition, a higher collagen content was found in left-sided colon cancer (LSCC). In future, this might aid in subclassification and therapeutic decisions or even in designing new therapy regimens by taking into account the differences between collagen fibres features between colon tumours located at different sides.

show abstract

Second‐harmonic imaging microscopy for identifying colorectal intraepithelial neoplasia

Cited by 3 publications

References 28 publications

Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa

Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa

Polarization Microscopy in Biomedical Applications

Label-free quantification of imaging features in the extracellular matrix of left and right-sided colon cancer tissues

Contact Info

Product

Resources

About