In vitro antioxidant activity of phenolic-enriched extracts from Zhangping Narcissus tea cake and their inhibition on growth and metastatic capacity of 4T1 murine breast cancer cells

Abstract

Phenolics, as the main bioactive compounds in tea, have been suggested to have potential in the prevention of various human diseases. However, little is known about phenolics and their bioactivity in Zhangping Narcissue tea cake which is considered the most special kind of oolong tea. To unveil its bioactivity, three phenolic-enriched extracts were obtained from Zhangping Narcissue tea cake using ethyl acetate, n-butanol, and water. Their main chemical compositions and in vitro bioactivity were analyzed by high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The ethyl acetate fraction (ZEF) consisted of higher content of phenolics, flavonoids, procyanidins, and catechin monomers (including epigallocatechin gallate (EGCG), epicatechin gallate (ECG), and gallocatechin gallate (GCG)) than n-butanol fraction (ZBF) and water fraction (ZWF). ZEF exhibited the strongest antioxidant capacity in vitro due to its abundant bioactive compounds. This was validated by Pearson correlation and hierarchical clustering analyses. ZEF also showed a remarkable inhibition on the growth, migration, and invasion of 4T1 murine breast cancer cells.

Antioxidant activity of alcohol aqueous extracts of Crypthecodinium cohnii and Schizochytrium sp.

“…Much research on the antioxidant effect of different microalgae has been carried out (Safafar et al, 2015;Singh et al, 2016). Polyphenol and flavonoid contents are commonly measured components referring to antioxidant activity (Pan et al, 2007;Saeed et al, 2012;Li et al, 2014;Narwal et al, 2014;Ying et al, 2017). Previous studies also verified the relationships between algal phenols and antioxidant capacity (Hajimahmoodi et al, 2010).…”

Effects of Tea Powder on the Cooking Properties, Antioxidative Potential and Volatile Profiles of Dried Noodles

“…Extraction of TP (MTP, GTP, and BTP) was performed according to Ying et al [ 12 ] with some modifications. Briefly, 1.5 g of tea powder was ultrasonically extracted twice with 250 mL of boiling distilled water in a boiling water bath for 1 h. Infusions were mixed and quickly filtered.…”

Role of Herbal Teas in Regulating Cellular Homeostasis and Autophagy and Their Implications in Regulating Overall Health

“…It can induce DNA damage and cleavage and play an inhibitory role in breast cancer cell growth, proliferation and tumorigenesis, thereby acting as a chemo-preventive agent against breast cancer [ 147 ]. The phenolics-rich fraction of oolong tea had more potent antioxidant activity, inhibiting the growth, migration, and invasion of breast cancer cells [ 148 ]. Fermentation of oolong tea using a fungus, Aspergillus sojae , can improve the gallic acid content of the tea extract, which could enhance the demethylation effects and a significant reduction in the nuclear abundances of DNMT1, DNMT3A, and DNMT3B in lung cancer cell lines [ 149 ].…”