A new water-soluble polythiophene derivative as a probe for real-time monitoring adenosine 5′-triphosphatase activity in lysosome of living cells

Highly Selective Fluorescence Sensing and Imaging of ATP Using a Boronic Acid Groups-Bearing Polythiophene Derivate

“…The titration of ATP into L buffer solution caused that the fluorescent intensities of the emission band centered at 570 nm decreased gradually with the increasing concentrations of ATP. The significant quenching of the emission of L could be explained by the formation of π-stacked aggregates of L induced by ATP, as demonstrated by UV-Vis and CD studied, quenching light emission via other nonradiative pathways [24,25]. The quenching constant K SV was determined according to the well-known Stern-Volmer equation [37].…”

“…From the changes in ATP concentrations-dependent fluorescence intensities (Figure S5, Supplementary Materials), the detection limit was estimated to be 2.07 × 10 −9 M based on the 3 σ /slope (where σ is the standard deviation of the background and slope is the sensitivity) [24,38]. Such a low detection limit should be very competitive with most of previously reported fluorescence or colorimetric probes for ATP [18,19,20,21,22,23,24,25] (Table S1, Supplementary Materials). The high sensitivity of L for ATP should be closely related to the strong synergetic interactions between L and ATP.…”

“…Among various CPs, polythiophene (PT) derivatives have received extensive attention because of their sensitive chain conformation transition responsive to external stimuli [7,8,18,19,20,21], providing unique advantages over other CPs on keeping the balance between simplicity (colorimetric mode) and sensitivity (fluorescence mode). In previous reports including ours, several PTs derivatives have been designed for the fluorescent detection of ATP with moderate selectivity and sensitivity [19,22,23,24,25]. These reported PT-based ATP probes contain generally cationic groups on their side chains, for example, quaternary ammonium [18,19,25,26], imidazolium [22], or phosphonium [23], to offer essential water solubility and recognition site for ATP.…”

“…In previous reports including ours, several PTs derivatives have been designed for the fluorescent detection of ATP with moderate selectivity and sensitivity [19,22,23,24,25]. These reported PT-based ATP probes contain generally cationic groups on their side chains, for example, quaternary ammonium [18,19,25,26], imidazolium [22], or phosphonium [23], to offer essential water solubility and recognition site for ATP. Thus, in most cases, electrostatic interaction is the primary driving force for the binding of polymer probes with ATP, resulting in these assays being sensitive to ionic strength.…”

Development of a fluorescent probe for detecting Al3+ in cooked wheaten food based on phosphonic acid group functionalized polythiophene derivatives

“…[27] Among these WSCPs, water-soluble polythiophenes (PTs) have received much attention due to their attractive optical properties, good photostability, and unique changes in their conformation. [28][29][30] To date, several kinds of PTs have been synthesized and applied to detect DNA, proteins, ATP, and metal ions. [30][31][32] In our previous studies, a water-soluble polythiophene derivative (poly{((thiophene-3,4-diylbis…”