Could ZnT8 antibodies replace ICA, GAD, IA2 and insulin antibodies in the diagnosis of type 1 diabetes?

Boudiaf, Ahlem; Bouziane, Djamila; Smara, M.; Meddour, Yanis; Haffaf, E.M.; Oudjit, Brahim; Mamouzi, S. Chaib; Aouichat-Bouguerra, S.

doi:10.1016/j.retram.2018.01.003

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…The mean age at the onset of diabetes was similar to an Indian population, and the distribution of gender was also similar [8]. There was a higher frequency of ZnT8A in males in the current study, which contrasts with a number of studies that reported a higher frequency in women or even documented no difference between genders with T1DM that was noticed by Eisenbarth et al probably because of difference in gender distribution in these studies [15,[19][20][21].…”

Section: Discussionsupporting

confidence: 53%

Association of Zinc Transporter-8 Autoantibody (ZnT8A) with Type 1 Diabetes Mellitus

Bhatty

Baig

Fawwad

et al. 2020

Cureus

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Background: Zinc transporter 8 autoantibody (ZnT8A), discovered through bioinformatics, is identified as another major biomarker for type 1 diabetes mellitus (T1DM), expanding the panel of diagnostic autoantibodies. The absence of standard autoantibodies in T1DM patients and the presence of ZnT8A in individuals before disease development has led the researchers to evaluate ZnT8A to gather information about the frequency and its association. Therefore, we aim to find out the concentration of ZnT8A and its association with T1DM. Methods: A case-control study with 25 type 1 diabetes mellitus patients and 25 first-degree relatives of cases as controls was conducted at Ziauddin University in collaboration with the Baqai Institute of Diabetology and Endocrinology (BIDE), Karachi. Demographic data were collected from patients on a standard questionnaire. Blood samples were collected, after approval from Ziauddin Ethics Review Committee, from subjects and serum was separated to estimate ZnT8A by using sandwich enzyme-linked immunosorbent assay (ELISA). Results: The mean age at diagnosis of T1DM patients was 13.40±5.05 years, and the duration of diabetes was 7.74±5.85 years. The frequency of ZnT8A was found higher in cases (19 (76%)) compared to controls (6 (24%)). ZnT8A concentrations were significantly higher in cases (13.82 ng/ml) compared to the controls (8.78 ng/ml; p= 0.024). The cutoff value of 9 ng/ml was selected for measuring sensitivity, specificity, and accuracy, which were determined as 76%, 76%, and 76%, respectively. Conclusions: ZnT8A was found significantly associated with T1DM. Subjects with ZnT8A values ≥ 9 ng/ml are 10 times more at risk to develop T1DM (p = 0.000).

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Section: Discussionsupporting

confidence: 53%

Association of Zinc Transporter-8 Autoantibody (ZnT8A) with Type 1 Diabetes Mellitus

Bhatty

Baig

Fawwad

et al. 2020

Cureus

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“…Autoantibodies in T1D are risk indicators for the diagnosis and prediction of the disease and surrogate markers for autoimmune diabetes [34–37]. The presence of several autoantibodies such as islet cell autoantibody (ICA) and GAD indicates an autoimmune pathogenic response to beta cells [38]. We measured the membrane variants of OX40 and OX40L in T1D individuals, which were reduced compared to HC.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of the Soluble Form of OX40 and OX40L Costimulatory Molecules but Reduction of the Membrane Form in Type 1 Diabetes (T1D)

Ding

et al. 2019

Journal of Immunology Research

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This study analyzed the expression of membrane OX40 and OX40L (mOX40 and mOX40L) and levels of soluble OX40 and OX40L (sOX40 and sOX40L) in T1D patients to determine their clinical significance. Peripheral blood (PB) was collected from patients with T1D and healthy control participants. Expression of mOX40 and mOX40L on immune cells was detected by flow cytometry. Levels of sOX40 and sOX40L in sera were measured by ELISA. We demonstrated for the first time enhanced sOX40 and sOX40L expression and reduced mOX40 and mOX40L levels in T1D patients which correlated with the clinical characteristics and inflammatory factors. These results suggest that OX40/OX40L signal may be promising biomarkers and associated with the pathogenesis of T1D.

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“…Lounici Boudiaf A, et al 18 ; Objective: Evaluate the prevalence of islets cells antibodies, glutamic acid decarboxylase, islet antigen type 2, insulin autoantibodies, and ZnT8 antibodies in young Algerian patients with T1DM; Type of study: cross-sectional study; Participants: 160 patients (74 males and 86 females) between 1 and 35 years old, diagnosed with type 1 diabetes; Results: ZnT8ab was positive in 70.3% females and 10.7% in males; Conclusion: “ZnT8ab is a good tool for differential diagnosis of T1DM”.…”

Section: Resultsmentioning

confidence: 99%

“…; Objective: Assessing the autoantibody profile in children with T1DM; Type of study: cross-sectional study; Participants: 92 subjects with T1DM (33 males, 59 females); Results: ZnT8 antibody were present in 20.65% subjects; Conclusion: "T1DM is associated with a high prevalence of autoantibodies and antibody negative T1DM is rare". Lounici Boudiaf A, et al18 ; Objective: Evaluate the prevalence of islets cells antibodies, glutamic acid decarboxylase, islet antigen type 2, insulin autoantibodies, and ZnT8 antibodies in young Algerian patients with T1DM; Type of study: cross-sectional study; Participants: 160 patients (74 males and 86 females) between 1 and 35 years old, diagnosed with type 1 diabetes; Results: ZnT8ab was positive in 70.3% females and 10.7% in males; Conclusion: "ZnT8ab is a good tool for differential diagnosis of T1DM".Source 3: Reference: Zecevic-Pasic L, et al 19 ; Objective: to analyze presence of T1DM-related autoantibodies; Type of study: cross-sectional study; Participants: 67 patients with T1DN (40 male and 27 female); Results: 36 (53,7%) cases were positive for Zn-T8; Conclusion: "Zn-T8 antibodies is the most frequently detected and is an important serological marker of T1DM" Source 4: Reference: Elmaoğulları S, et al 20 ; Objective: to investigate the prevalence of ZnT8A in Turkish children with new onset T1DM; Type of study: cross-sectional study; Participants: 84 patients diagnosed with T1DM and 50 healthy children without any autoimmune diseases; Results: ZnT8A positivity was detected in 58% of the patients with new onset T1DM and 8% of the control group; Conclusion: "ZnT8 measurement should be more widespread for clarifying the etiology in T1DM". Reference: Bhola S, et al 21 ; Objective: To establish the frequency of ZnT8 autoantibodies in black South Africans diagnosed with T1DM, and investigate potential correlations between ZnT8 autoantibody positivity, age at diagnosis, and duration of the disease; Type of study: cross-sectional study; Participants: Patients with T1DM (n = 183) and controls (n = 49) Results: The prevalence of ZnT8 autoantibody positivity was 17.5 % (32 of 183) in participants with T1DM and 27.3 % (6 of 22) in newly diagnosed participants; Conclusion: "The greater the numbers of autoantibodies present in an individual the earlier the age at diagnosis".…”

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confidence: 99%

Evaluating the Diagnostic Accuracy of Anti- Zinc Transporter 8 Antibodies in Type 1 Diabetes: A Systematic Review and Meta-Analysis

de Oliveira Andrade,

de Oliveira,

Rocha

et al. 2024

Preprint

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BackgroundThe association between autoantibodies and the risk of type 1 diabetes mellitus (T1DM) is well established. However, there is still a lack of quantitative insight into the role of anti-zinc transporter 8 (anti-ZnT8) antibodies and their efficacy in T1DM diagnosis.ObjectiveTo conduct a systematic review and meta-analysis on the association between anti-ZnT8 autoantibodies and the risk of T1DM.MethodsRelevant studies were retrieved from the PubMed database and analyzed using a fixed or random-effects model.ResultsOut of 211 screened articles, 23 studies were selected and a total of 14,172 patients were included in the analysis. Our pooled analysis revealed that anti-ZnT8 autoantibody expression was significantly associated with an increased risk of T1DM development in both children and adults [OR 1.14 (95% CI: 1.12-1.16); P = 0.07].ConclusionOur systematic review and meta-analysis provides robust evidence supporting a significant association between anti-ZnT8 autoantibody positivity and an increased risk of T1DM development.

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Could ZnT8 antibodies replace ICA, GAD, IA2 and insulin antibodies in the diagnosis of type 1 diabetes?

Cited by 12 publications

References 36 publications

Association of Zinc Transporter-8 Autoantibody (ZnT8A) with Type 1 Diabetes Mellitus

Association of Zinc Transporter-8 Autoantibody (ZnT8A) with Type 1 Diabetes Mellitus

Enhancement of the Soluble Form of OX40 and OX40L Costimulatory Molecules but Reduction of the Membrane Form in Type 1 Diabetes (T1D)

Evaluating the Diagnostic Accuracy of Anti- Zinc Transporter 8 Antibodies in Type 1 Diabetes: A Systematic Review and Meta-Analysis

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