Isolation, characterization, and identification of potential Diuron-degrading bacteria from surface sediments of Port Klang, Malaysia

Abstract

Diuron is an alternative biocide suggested to replace organotin in formulating antifouling paints to be applied on water-going vessels hull. However, it is potentially harmful to various non-targeted marine organisms due to its toxic properties. Present study aimed to isolate, screen and identify the potential of Diuron-degrading bacteria collected from the marine sediments of Port Klang, Malaysia. Preliminary screening was conducted by exposing isolated bacteria to 430ng/L (background level), followed by 600ng/L and 1000ng/L of Diuron concentrations. Nine bacteria colonies survived the exposure of the above concentrations. However, only two strains can tolerate to survive up to 1000μg/L, which were then characterised and identified using phenotypic tests and the standard 16S rRNA molecular identification. The strains were identified as Comamonas jiangduensis SZZ 10 and Bacillus aerius SZZ 19 (GenBank accession numbers: KU942479 and KU942480, respectively). Both strains have the potential of Diuron biodegradation for future use.

Methylene blue decolorizing bacteria isolated from water sewage in Yogyakarta, Indonesia

“…C. terrae strain A3-3 isolated from tannery wastes and agricultural soils have shown to potentially oxidize arsenite to arsenate (Chitpirom et al 2009). C. jiangduensis SZZ 10 has the potential to degrade the diuron herbicide (Hanapiah et al 2018). Despite the genus Comamonas involvement in several bioremediations, there is currently no study on the ability of C. aquatica to decolorize dyes.…”

Embryonic toxicity of 3,4-dichloroaniline (3,4-DCA) on Javanese medaka (Oryzias javanicus Bleeker, 1854)

“…It is a highly persistent chemical as a result of its high stability and low volatility [ 4 ]. It is frequently used as phenylurea herbicides (diuron and linuron) and acylanilide herbicide (propanil) [ 4 , 5 ]. Chemical industries use 3,4-DCA as an intermediary in the production of azo dyes, paints, cosmetics [ [6] , [7] , [8] ].…”

Distribution of Benthic Macroinvertebrates in Seafloor Northward of Pulau Indah, Klang

“…This study can help us in identifying possible ecological stresses in the region and suggesting appropriate benthic bioindicator species inhabiting it, in which their population may indicate the current status of ecosystem health of the habitat. The outcome of this study may put ways for other specialized studies on sediments at waters of Port Klang such as heavy metal (ELturk et al, 2019), oil spill (Omar et al, 2018) and bacterial activities in response to presence of certain benthic organisms inhabiting the sediment (Hanapiah et al, 2018).…”